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Glycolysis as the Central Core of Fermentation

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Book cover Anaerobes in Biotechnology

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 156))

Abstract

The increasing concerns of greenhouse gas emissions have increased the interest in dark fermentation as a means of productions for industrial chemicals, especially from renewable cellulosic biomass. However, the metabolism, including glycolysis, of many candidate organisms for cellulosic biomass conversion through consolidated bioprocessing is still poorly understood and the genomes have only recently been sequenced. Because a variety of industrial chemicals are produced directly from sugar metabolism, the careful understanding of glycolysis from a genomic and biochemical point of view is essential in the development of strategies for increasing product yields and therefore increasing industrial potential. The current review discusses the different pathways available for glycolysis along with unexpected variations from traditional models, especially in the utilization of alternate energy intermediates (GTP, pyrophosphate). This reinforces the need for a careful description of interactions between energy metabolites and glycolysis enzymes for understanding carbon and electron flux regulation.

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Abbreviations

ABE:

Acetone–Butanol–Ethanol process

ADP:

Adenosine diphosphate

ATP:

Adenosine triphosphate

ED:

Entner–Doudoroff pathway

EMP:

Embden–Meyerhof–Parnas pathway

GTP:

Guanosine triphosphate

NADH:

Nicotinamide adenine dinucleotide

NADPH:

Nicotinamide adenine dinucleotide phosphate

PPi:

Pyrophosphate

PPP:

Pentose phosphate pathway

TCA:

Tricarboxylic acid cycle

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Acknowledgements

This work was funded through the Genome Canada-funded Microbial Genomics for Biofuels and Co-products Biorefineries project and through NSERC Discovery Grant (RGPIN-2014-06173).

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  1. Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2

    M. Taillefer & R. Sparling

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  1. M. Taillefer
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  1. Biotechnology, Department of Chemistry Center for Chemistry & Chemical Engineering, Lund University, Lund, Sweden

    Rajni Hatti-Kaul

  2. Biotechnology, Department of Chemistry Center for Chemistry & Chemical Engineering, Lund University, Lund, Sweden

    Gashaw Mamo

  3. Biotechnology, Department of Chemistry Center for Chemistry & Chemical Engineering, Lund University, Lund, Sweden

    Bo Mattiasson

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Taillefer, M., Sparling, R. (2016). Glycolysis as the Central Core of Fermentation. In: Hatti-Kaul, R., Mamo, G., Mattiasson, B. (eds) Anaerobes in Biotechnology. Advances in Biochemical Engineering/Biotechnology, vol 156. Springer, Cham. https://doi.org/10.1007/10_2015_5003

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