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Cell Culture Process Operations for Recombinant Protein Production

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Book cover Mammalian Cell Cultures for Biologics Manufacturing

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 139))

Abstract

The market for protein therapeutics has grown significantly over the past two decades and the pace of development continues to increase. It is a challenge to the industry to maintain the desired quality attributes while accelerating delivery to patients, reducing the cost of goods, and providing production flexibility. Efficient manufacturing scale production of protein therapeutics is required to continue to meet the needs of the patients and stockholders. This chapter describes batch, fed-batch, and perfusion processes and their utilization in the production of monoclonal antibodies and other therapeutic proteins. In addition, we have provided detailed discussions of the ongoing challenges of lactate metabolism and the future prospects of process monitoring and control.

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Authors and Affiliations

  1. Bristol-Myers Squibb, Manufacturing Sciences and Technology, 38 Jackson Road, Devens, MA, 01434, USA

    Susan Abu-Absi & Sen Xu

  2. Bristol-Myers Squibb, Manufacturing Sciences and Technology, 6000 Thompson Road, East Syracuse, NY, 13057, USA

    Hugh Graham, Nimish Dalal, Marcus Boyer & Kedar Dave

Authors
  1. Susan Abu-Absi
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  2. Sen Xu
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  3. Hugh Graham
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  4. Nimish Dalal
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  5. Marcus Boyer
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  6. Kedar Dave
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Corresponding author

Correspondence to Susan Abu-Absi .

Editor information

Editors and Affiliations

  1. WuXi AppTec Co., Ltd, Shanghai, People's Republic of China

    Weichang Zhou

  2. Alexion Pharmaceuticals, Cheshire, USA

    Anne Kantardjieff

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Abu-Absi, S., Xu, S., Graham, H., Dalal, N., Boyer, M., Dave, K. (2013). Cell Culture Process Operations for Recombinant Protein Production. In: Zhou, W., Kantardjieff, A. (eds) Mammalian Cell Cultures for Biologics Manufacturing. Advances in Biochemical Engineering/Biotechnology, vol 139. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2013_252

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