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Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism

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Book cover Biotechnology of Hairy Root Systems

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 134))

Abstract

It is commonly accepted that the plant pathogens Agrobacterium rhizogenes and Agrobacterium tumefaciens, acting via their T-DNA oncogenes, disturb hormone metabolism or hormone perception pathways in plants, thereby attaining their aim of successful pathogenesis. In this work, we summarize recent data on the A. rhizogenes rolC and rolB oncogenes in comparison to the A. tumefaciens 6b oncogene with respect to their effects on the physiology of transformed cells. The newly discovered functions of the rol genes include the modulation of secondary metabolism, the modulation of levels of intracellular ROS and stress resistance of transformed cells, changed sucrose metabolism, and the inhibition of programmed cell death. We show that the rol genes do not have suppressive effects on plant innate immunity; rather, these genes activate plant defense reactions. The existence of not only the hormone-related mechanism of pathogenicity but also the defense-related mechanism of pathogenicity during plant-Agrobacterium interactions is suggested.

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Abbreviations

ROS:

Reactive oxygen species

siRNA:

Small interfering RNA

miRNA:

MicroRNA

RISC:

RNA-induced silencing complex

dsDNA:

Double-stranded DNA

H2DCF-DA:

2,7-dichlorofluorescein diacetate

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Acknowledgments

This work was supported by grants from the Russian Foundation for Basic Research and by the Grant Program “Molecular and Cell Biology” of the Russian Academy of Sciences.

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Authors and Affiliations

  1. Institute of Biology and Soil Science, Far East Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia

    Victor P. Bulgakov, Yuri N. Shkryl, Galina N. Veremeichik, Tatiana Y. Gorpenchenko & Yuliya V. Vereshchagina

  2. Far Eastern Federal University, Vladivostok, 690950, Russia

    Victor P. Bulgakov

Authors
  1. Victor P. Bulgakov
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  2. Yuri N. Shkryl
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  3. Galina N. Veremeichik
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  4. Tatiana Y. Gorpenchenko
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  5. Yuliya V. Vereshchagina
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Correspondence to Victor P. Bulgakov .

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Editors and Affiliations

  1. Faculty of Life and Social Science, Swinburne University of Technology, Hawthorn, Melbourne, VIC, Australia

    Pauline M Doran

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Bulgakov, V.P., Shkryl, Y.N., Veremeichik, G.N., Gorpenchenko, T.Y., Vereshchagina, Y.V. (2013). Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism. In: Doran, P. (eds) Biotechnology of Hairy Root Systems. Advances in Biochemical Engineering/Biotechnology, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2013_179

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