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Mesenchymal Stem Cells - Basics and Clinical Application I pp 153–176Cite as

Proteomics Approaches in the Identification of Molecular Signatures of Mesenchymal Stem Cells

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Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 129))

Abstract

Mesenchymal stem cells (MSCs) are undifferentiated, multi-potent stem cells with the ability to renew. They can differentiate into many types of terminal cells, such as osteoblasts, chondrocytes, adipocytes, myocytes, and neurons. These cells have been applied in tissue engineering as the main cell type to regenerate new tissues. However, a number of issues remain concerning the use of MSCs, such as cell surface markers, the determining factors responsible for their differentiation to terminal cells, and the mechanisms whereby growth factors stimulate MSCs. In this chapter, we will discuss how proteomic techniques have contributed to our current knowledge and how they can be used to address issues currently facing MSC research. The application of proteomics has led to the identification of a special pattern of cell surface protein expression of MSCs. The technique has also contributed to the study of a regulatory network of MSC differentiation to terminal differentiated cells, including osteocytes, chondrocytes, adipocytes, neurons, cardiomyocytes, hepatocytes, and pancreatic islet cells. It has also helped elucidate mechanisms for growth factor–stimulated differentiation of MSCs. Proteomics can, however, not reveal the accurate role of a special pathway and must therefore be combined with other approaches for this purpose. A new generation of proteomic techniques have recently been developed, which will enable a more comprehensive study of MSCs.

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Authors and Affiliations

  1. Institute of Health and Biomedical Innovation Queensland University of Technology, 60 Musk Avenue, Kelvin Grove Brisbane, QLD, 4059, Australia

    Yin Xiao & Jiezhong Chen

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  1. Yin Xiao
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  2. Jiezhong Chen
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Correspondence to Yin Xiao .

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Editors and Affiliations

  1. Abt. für Plastische und Handchirurgie, Medizinische Hochschule Hannover, Carl-Neubergstr. 1, Hannover, 30625, Germany

    Birgit Weyand

  2. Integrated Department of Oncology, Haematology and Respiratory Diseases, University of Modena, Modena, Italy

    Massimo Dominici

  3. Klinik für Frauenheilkunde und Geburtshi, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, Hannover, 30625, Germany

    Ralf Hass

  4. Klinik für Immunologie und Rheumatologie, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, Hannover, 30625, Niedersachsen, Germany

    Roland Jacobs

  5. Department für Biotechnologie, Universität für Bodenkultur, Muthgasse 18, Wien, 1190, Austria

    Cornelia Kasper

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© 2012 Springer-Verlag Berlin Heidelberg

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Xiao, Y., Chen, J. (2012). Proteomics Approaches in the Identification of Molecular Signatures of Mesenchymal Stem Cells. In: Weyand, B., Dominici, M., Hass, R., Jacobs, R., Kasper, C. (eds) Mesenchymal Stem Cells - Basics and Clinical Application I. Advances in Biochemical Engineering/Biotechnology, vol 129. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2012_143

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