Abstract
The final goal of our group is to establish the missing link between chemical reaction and mechanical event in molecular motors. To achieve this, we have developed advanced versions of conventional optical microscopes and applied them into single-molecule techniques. In this chapter we present two studies: one is about the kinesin-microtubule system and the other F1-ATPase. These techniques are applicable to other molecular machines, hopefully in more sophisticated ways, and we hope to investigate this in future studies.
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References
Nishizaka T, Yagi T, Tanaka Y, Ishiwata S (1993) Nature 361:269
Nishizaka T, Miyata H, Yoshikawa H, Ishiwata S, Kinosita K Jr (1995) Biophys J 68:75s
Nishizaka T, Seo R, Tadakuma H, Kinosita K Jr, Ishiwata S (2000) Biophys J 79:962
Yajima J, Mizutani K, Nishizaka T (2008) Nat Struct Mol Biol 15:1119
Nishizaka T, Oiwa K, Noji H, Kimura S, Muneyuki E, Yoshida M, Kinosita K Jr (2004) Nat Struct Mol Biol 11:142
Adachi K, Oiwa K, Nishizaka T, Furuike S, Noji H, Itoh H, Yoshida M, Kinosita K Jr (2007) Cell 130:309
Masaike T, Koyama-Horibe F, Oiwa K, Yoshida M, Nishizaka T (2008) Nat Struct Mol Biol 15:1326
Yajima J, Cross RA (2005) Nat Chem Biol 1:338
Ray S, Mayhofer E, Milligan RA, Howard J (1993) J Cell Biol 121:1083
Funatsu T, Harada Y, Tokunaga M, Saito K, Yanagida T (1995) Nature 374:555
Noji H, Yasuda R, Yoshida M, Kinosita K Jr (1997) Nature 386:299
Abrahams JP, Leslie AG, Lutter R, Walker JE (1994) Nature 370:621
Shimabukuro K, Yasuda R, Muneyuki E, Hara KY, Kinosita K Jr, Yoshida M (2003) Proc Natl Acad Sci USA 100:14731
Yasuda R, Noji H, Yoshida M, Kinosita K Jr, Itoh H (2001) Nature 410:898
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Nishizaka, T. (2010). Molecular Bio-Motors in Living Cells. In: Endo, I., Nagamune, T. (eds) Nano/Micro Biotechnology. Advances in Biochemical Engineering / Biotechnology, vol 119. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2010_69
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DOI: https://doi.org/10.1007/10_2010_69
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