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Biosystems Engineering I pp 1–19Cite as

Integration of Systems Biology with Bioprocess Engineering: l-Threonine Production by Systems Metabolic Engineering of Escherichia Coli

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Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 120))

Abstract

Random mutation and selection or targeted metabolic engineering without consideration of its impact on the entire metabolic and regulatory networks can unintentionally cause genetic alterations in the region, which is not directly related to the target metabolite. This is one of the reasons why strategies for developing industrial strains are now shifted towards targeted metabolic engineering based on systems biology, which is termed systems metabolic engineering. Using systems metabolic engineering strategies, all the metabolic engineering works are conducted in systems biology framework, whereby entire metabolic and regulatory networks are thoroughly considered in an integrated manner. The targets for purposeful engineering are selected after all possible effects on the entire metabolic and regulatory networks are thoroughly considered. Finally, the strain, which is capable of producing the target metabolite to a high level close to the theoretical maximum value, can be constructed. Here we review strategies and applications of systems biology successfully implemented on bioprocess engineering, with particular focus on developing l-threonine production strains of Escherichia coli.

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Acknowledgments

Our work described in this chapter was supported by the Korea–Australia Collaborative Research Project on the Development of Sucrose-Based Bioprocess Platform (N02071165) from the Ministry of Knowledge Economy and by the Korean Systems Biology Program from the Ministry of Education, Science and Technology (No. M10309020000-03B5002-00000). Further support by the World Class University (WCU) program from the Ministry of Education, Science and Technology, LG Chem Chair Professorship and Microsoft is appreciated.

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Authors and Affiliations

  1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Sang Yup Lee & Jin Hwan Park

  2. BioProcess Engineering Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Sang Yup Lee & Jin Hwan Park

  3. Department of Bio and Brain Engineering and Bioinformatics Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Sang Yup Lee

  4. Department of Biological Sciences, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Sang Yup Lee

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  1. Sang Yup Lee
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Correspondence to Sang Yup Lee .

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Editors and Affiliations

  1. Inst. Bioverfahrenstechnik, TU Braunschweig, Gaußstraße 17, Braunschweig, 38106, Germany

    Christoph Wittmann

  2. Inst. Bioverfahrenstechnik, TU Braunschweig, Gaußstraße 17, Braunschweig, 38106, Germany

    Rainer Krull

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Lee, S.Y., Park, J.H. (2010). Integration of Systems Biology with Bioprocess Engineering: l-Threonine Production by Systems Metabolic Engineering of Escherichia Coli . In: Wittmann, C., Krull, R. (eds) Biosystems Engineering I. Advances in Biochemical Engineering / Biotechnology, vol 120. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2009_57

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