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Biosystems Engineering II pp 45–70Cite as

Impact of Profiling Technologies in the Understanding of Recombinant Protein Production

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Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 121))

Abstract

Since expression profiling methods have been available in a high throughput fashion, the implication of these technologies in the field of biotechnology has increased dramatically. Microarray technology is one such unique and efficient methodology for simultaneous exploration of expression levels of numerous genes. Likewise, two-dimensional gel electrophoresis or multidimensional liquid chromatography coupled with mass spectrometry are extensively utilised for studying expression levels of numerous proteins. In the field of biotechnology these highly parallel analytical methods have paved the way to study and understand various biological phenomena depending on expression patterns. The next phenomenological level is represented by the metabolome and the (metabolic) fluxome. However, this chapter reviews gene and protein profiling and their impact on understanding recombinant protein production. We focus on the computational methods utilised for the analyses of data obtained from these profiling technologies as well as prominent results focusing on recombinant protein expression with Escherichia coli. Owing to the knowledge accumulated with respect to cellular signals triggered during recombinant protein production, this field is on the way to design strategies for developing improved processes. Both gene and protein profiling have exhibited a handful of functional categories to concentrate on in order to identify target genes and proteins, respectively, involved in the signalling network with major impact on recombinant protein production.

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Author information

Authors and Affiliations

  1. International NRW Graduate School in Bioinformatics and Genome Research, CeBiTec, Bielefeld University, 33594, Bielefeld, Germany

    Chandran Vijayendran

  2. Faculty of Technology, Chair of Fermentation Engineering, Bielefeld University, 33594, Bielefeld, Germany

    Erwin Flaschel

Authors
  1. Chandran Vijayendran
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  2. Erwin Flaschel
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Correspondence to Erwin Flaschel .

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Editors and Affiliations

  1. Inst. Bioverfahrenstechnik, TU Braunschweig, Gaußstraße 17, Braunschweig, 38106, Germany

    Christoph Wittmann

  2. Inst. Bioverfahrenstechnik, TU Braunschweig, Gaußstraße 17, Braunschweig, 38106, Germany

    Rainer Krull

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Vijayendran, C., Flaschel, E. (2010). Impact of Profiling Technologies in the Understanding of Recombinant Protein Production. In: Wittmann, C., Krull, R. (eds) Biosystems Engineering II. Advances in Biochemical Engineering / Biotechnology, vol 121. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2009_56

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