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Surface Functionalization for Protein and Cell Patterning

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Book cover Whole Cell Sensing Systems I

Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 117))

Abstract

The interaction of biological systems with synthetic material surfaces is an important issue for many biological applications such as implanted devices, tissue engineering, cell-based sensors and assays, and more generally biologic studies performed ex vivo. To ensure reliable outcomes, the main challenge resides in the ability to design and develop surfaces or artificial micro-environment that mimic ‘natural environment’ in interacting with biomolecules and cells without altering their function and phenotype. At this effect, microfabrication, surface chemistry and material science play a pivotal role in the design of advanced in-vitro systems for cell culture applications. In this chapter, we discuss and describe different techniques enabling the control of cell-surface interactions, including the description of some techniques for immobilization of ligands for controlling cell-surface interactions and some methodologies for the creation of well confined cell rich areas.

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Acknowledgements

This work was supported by Action 4,221: “Nanobiotechnologies for Health” of the Joint Research Centre. We thanks Dr S. Belkin for the bacteria culture experiments.

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Authors and Affiliations

  1. European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Via E.Fermi, 2749 TP203, 21027, Ispra, Varese, Italy

    Pascal Colpo, Ana Ruiz, Laura Ceriotti & François Rossi

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  1. Pascal Colpo
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  2. Ana Ruiz
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  3. Laura Ceriotti
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  4. François Rossi
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Correspondence to Pascal Colpo .

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Editors and Affiliations

  1. , Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel

    Shimshon Belkin

  2. , College of Life Sciences & Biotechnology, Korea University, Amam-dong 5-ga, Seoul, 136-701, Korea, Republic of (South Korea)

    Man Bock Gu

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Colpo, P., Ruiz, A., Ceriotti, L., Rossi, F. (2009). Surface Functionalization for Protein and Cell Patterning. In: Belkin, S., Gu, M. (eds) Whole Cell Sensing Systems I. Advances in Biochemical Engineering / Biotechnology, vol 117. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2009_2

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