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Dynamic Visualization of Cellular Signaling

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Nano/Micro Biotechnology

Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 119))

Abstract

Our understanding of cellular signaling is critically dependent on our ability to visualize and quantify specific signaling events with high spatial and temporal resolution in the cellular context. Over the past decade or so, biosensors based on fluorescent proteins and fluorescence resonance energy transfer (FRET) have emerged as one major class of fluorescent probes that are capable of tracking a variety of cellular signaling events, such as second messenger dynamics and enzyme activation/activity, in time and space. Here we review recent advances in the development of such biosensors and some biological insights revealed by these biosensors in living cells, tissue, and organisms.

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Author information

Authors and Affiliations

  1. Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Qiang Ni

  2. Department of Pharmacology and Molecular Sciences, The Solomon H. Snyder Departments of Neuroscience and Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Jin Zhang

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  1. Qiang Ni
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  2. Jin Zhang
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Correspondence to Jin Zhang .

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Editors and Affiliations

  1. Saitama Industrial Technology Center, Kamiaoki 3-12-18, Kawaguchi-shi, Saitama, 333-0844, Japan

    Isao Endo

  2. School of Engineering, Dept. Bioengineering, University of Tokyo, Hongo 7-3-1, Tokyo, 113-8656, Japan

    Teruyuki Nagamune

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© 2009 Springer-Verlag Berlin Heidelberg

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Ni, Q., Zhang, J. (2009). Dynamic Visualization of Cellular Signaling. In: Endo, I., Nagamune, T. (eds) Nano/Micro Biotechnology. Advances in Biochemical Engineering / Biotechnology, vol 119. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2008_48

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