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Large Scale Culture of Ginseng Adventitious Roots for Production of Ginsenosides

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Biotechnology in China I

Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 113))

Abstract

Ginseng (Panax ginseng C. A. Meyer) is one of the most famous oriental medicinal plants used as crude drugs in Asian countries, and now it is being used worldwide for preventive and therapeutic purposes. Among diverse constituents of ginseng, saponins (ginsenosides) have been found to be major components responsible for their biological and pharmacological actions. On the other hand, difficulties in the supply of pure ginsenosides in quantity prevent the development of ginseng for clinical medicines. Cultivation of ginseng in fields takes a long time, generally 5–7 years, and needs extensive effort regarding quality control since growth is susceptible to many environmental factors including soil, shade, climate, pathogens and pests. To solve the problems, cell and tissue cultures have been widely explored for more rapid and efficient production of ginseng biomass and ginsenosides. Recently, cell and adventitious root cultures of P. ginseng have been established in large scale bioreactors with a view to commercial application. Various physiological and engineering parameters affecting the biomass production and ginsenoside accumulation have been investigated. Advances in adventitious root cultures including factors for process scale-up are reviewed in this chapter. In addition, biosafety analyses of ginseng adventitious roots are also discussed for real application.

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Abbreviations

2, 4-D:

2, 4-Dichloraophenoxy acetic acid

DW:

Dry weight

FW:

Fresh weight

IAA:

Indole-3-acetic acidM

IBA:

Indole-3-butyric acid

MJ:

Methyl jasmonate

MS medium:

Murashige and Skoog medium

NAA:

α-Naphthalene acetic acid

vvm:

Air volume per medium volume per minute

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Acknowledgment

This work was financially supported by the Ministry of Education and Human Resources Development, the Ministry of Commerce, Industry, and Energy and the Ministry of Labor. One of the authors (H.N. Murthy) is grateful to Korean Federation of Science and Technology Societies (KOFST) for the award of Brain Pool Fellowship. JJZ thanks the funds from the Shanghai Leading Academic Discipline Project (project # B203).

Author information

Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Kee-Yoeup Paek, Hosakatte Niranjana Murthy & Eun-Joo Hahn

  2. Department of Botany, Karnatak University, Dharwad, 580 003, India

    Hosakatte Niranjana Murthy

  3. School of Life Sciences and Biotechnology, Key Laboratory of Microbial Metabolism (Ministry of Education), Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai, 200240, China

    Jian-Jiang Zhong

Authors
  1. Kee-Yoeup Paek
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  2. Hosakatte Niranjana Murthy
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  3. Eun-Joo Hahn
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  4. Jian-Jiang Zhong
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Corresponding authors

Correspondence to Kee-Yoeup Paek or Jian-Jiang Zhong .

Editor information

Editors and Affiliations

  1. College of Life Science & Biotechnology, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, Minhang, 200240, China, People's Republic

    Jian-Jiang Zhong

  2. Dept. Bioscience & Bioengineering, Dalian University of Technology, Dalian, 116023, China, People's Republic

    Feng-Wu Bai

  3. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China, People's Republic

    Wei Zhang

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© 2009 Springer-Verlag London

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Paek, KY., Murthy, H.N., Hahn, EJ., Zhong, JJ. (2009). Large Scale Culture of Ginseng Adventitious Roots for Production of Ginsenosides. In: Zhong, JJ., Bai, FW., Zhang, W. (eds) Biotechnology in China I. Advances in Biochemical Engineering / Biotechnology, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2008_31

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