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Mechanical Strain Using 2D and 3D Bioreactors Induces Osteogenesis: Implications for Bone Tissue Engineering

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Bioreactor Systems for Tissue Engineering

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 112))

Fracture healing is a complicated process involving many growth factors, cells, and physical forces. In cases, where natural healing is not able, efforts have to be undertaken to improve healing. For this purpose, tissue engineering may be an option. In order to stimulate cells to form a bone tissue several factors are needed: cells, scaffold, and growth factors. Stem cells derived from bone marrow or adipose tissues are the most useful in this regard. The differentiation of the cells can be accelerated using mechanical stimulation. The first part of this chapter describes the influence of longitudinal strain application. The second part uses a sophisticated approach with stem cells on a newly developed biomaterial (Sponceram) in a rotating bed bioreactor with the administration of bone morphogenetic protein-2. It is shown that such an approach is able to produce bone tissue constructs. This may lead to production of larger constructs that can be used in clinical applications.

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Authors and Affiliations

  1. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstraβe 13, Vienna, A-1200, Austria

    M. van Griensven, S. Diederichs, A. Peterbauer & S. Wolbank

  2. Institut für Technische Chemie, Leibniz University Hannover, Callinstraβe 3, Hannover, 30167, Germany

    S. Diederichs, S. Roeker, S. Boehm, D. Riechers, F. Stahl & C. Kasper

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  1. M. van Griensven
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  2. S. Diederichs
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  9. C. Kasper
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Correspondence to M. van Griensven .

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Editors and Affiliations

  1. Institute of Technical Chemistry, University of Hannover, Callinstraβe 3, Hannover, 30167, Germany

    Cornelia Kasper

  2. Ludwig Boltzmann Institut für, Klinische und Experimentelle, Traumatologie Donaueschingenstr. 13, Wien, 1200, Austria

    Martijn van Griensven

  3. TU Hamburg-Harburg, Institut für Biotechnologie und Verfahrenstechnik Denickestr. 15, Hamburg, 21073, Germany

    Ralf Pörtner

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van Griensven, M. et al. (2009). Mechanical Strain Using 2D and 3D Bioreactors Induces Osteogenesis: Implications for Bone Tissue Engineering. In: Kasper, C., van Griensven, M., Pörtner, R. (eds) Bioreactor Systems for Tissue Engineering. Advances in Biochemical Engineering/Biotechnology, vol 112. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2008_14

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