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DNA Microarrays

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Book cover Biosensing for the 21st Century

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 109))

Abstract

Microarray technology provides new analytical devices that allow the parallel and simultaneous detection of several thousands of probes within one sample. Microarrays, sometimes called DNA chips, are widely used in gene-expression analysis, genotyping of individuals, analysis of point mutations and single nucleotide polymorphisms (SNP) as well as other genomic or transcriptomic variations. In this chapter we give a survey of common microarray manufacturing, the selection of support material, immobilisation and hybridisation and the detection with labelled complementary strands. However, DNA arrays may also serve as the basis for more complex analysis based on the action of enzymes on the immobilized templates. This property gives DNA microarrays the potential for being the template for whole PCR and transcription experiments with high parallelism, as will be discussed in the last section of this chapter.

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Authors and Affiliations

  1. Fraunhofer Institute for Biomedical Engineering, Branch Potsdam, Am Mühlenberg 13, 14476, Potsdam, Germany

    Frank F. Bier, Markus von Nickisch-Rosenegk, Eva Ehrentreich-Förster, Edda Reiß, Jörg Henkel, Rothin Strehlow & Dennie Andresen

Authors
  1. Frank F. Bier
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  2. Markus von Nickisch-Rosenegk
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  3. Eva Ehrentreich-Förster
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  4. Edda Reiß
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  5. Jörg Henkel
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  6. Rothin Strehlow
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  7. Dennie Andresen
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Corresponding author

Correspondence to Frank F. Bier .

Editor information

Reinhard Renneberg Fred Lisdat

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© 2007 Springer-Verlag Berlin Heidelberg

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Bier, F.F. et al. (2007). DNA Microarrays. In: Renneberg, R., Lisdat, F. (eds) Biosensing for the 21st Century. Advances in Biochemical Engineering/Biotechnology, vol 109. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2007_087

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