Abstract
Semiconductor nanoparticles (NPs) or quantum dots (QDs) exhibit unique photophysical properties reflected by size-controlled fluorescence, high fluorescence quantum yields, and stability against photobleaching. These properties are utilized by applying the QDs as optical labels for the multiplexed analysis of immunocomplexes and DNA hybridization. Also, semiconductor QDs are used to probe biocatalytic transformations. The time-dependent replication or telomerization of nucleic acids, the oxidation of phenol derivatives by tyrosinase, and the hydrolytic cleavage of peptides by proteases are probed by using fluorescence resonance energy transfer or photoinduced electron transfer. The photoexcitation of semiconductor NP–biomolecule hybrids associated with electrodes enables the photoelectrochemical transduction of biorecognition events or biocatalytic transformations. This is exemplified with the generation of photocurrents by duplex DNA assemblies bridging CdS NPs to electrodes, and by the formation of photocurrents as a result of biocatalyzed transformations, or redox protein-mediated electron transfer in the presence of the NPs.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Brus LE (1991) Appl Phys A 53:465
Alivisatos AP (1996) Science 271:933
Grieve K, Mulvaney P, Grieser F (2000) Curr Opin Colloid Interface Sci 5:168
Alivisatos AP (2004) Nat Biotechnol 22:47
Nirmal M, Brus LE (1999) Acc Chem Res 32:407
Medintz IL, Uyeda HT, Goldman ER, Mattoussi H (2005) Nat Mater 4:435
Chan WCW, Maxwell DJ, Gao X, Bailey RE, Han M, Nie S (2002) Curr Opin Biotechnol 13:40
Kamat PV (2007) J Phys Chem C 111:2834
Sapsford KE, Pons T, Medintz IL, Mattoussi H (2006) Sensors 6:925
Costa-Fernandez JM, Pereiro R, Sanz-Medel A (2006) Trends Anal Chem 25:207
Klostranec JM, Chan WCW (2006) Adv Mater 18:1953
Murphy CJ (2002) Anal Chem 74:520A
Goldman ER, Balighian ED, Mattoussi H, Kuno MK, Mauro JM, Tran PT, Anderson GP (2002) J Am Chem Soc 124:6378
Goldman ER, Anderson GP, Tran PT, Mattoussi H, Charles PT, Mauro JM (2002) Anal Chem 74:841
Goldman ER, Clapp AR, Anderson GP, Uyeda HT, Mauro JM, Medintz IL, Mattoussi H (2004) Anal Chem 76:684
Yang L, Li Y (2006) Analyst 131:394
Hoshino A, Fujioka K, Manabe N, Yamay S, Goto Y, Yasuhara M, Yamamoto K (2005) Microbiol Immunol 49:461
Gerion D, Chen F, Kannan B, Fu A, Parak WJ, Chen DJ, Majumdar A, Alivisatos AP (2003) Anal Chem 75:4766
Liang RQ, Li W, Li Y, Tan CY, Li JX, Jin YX, Ruan KC (2005) Nucl Acids Res 33:e17
Pathak S, Choi SK, Arnheim N, Thompson ME (2001) J Am Chem Soc 123:4103
Xiao Y, Barker PE (2004) Nucleic Acids Res 32:e28
Chan PM, Yuen T, Ruf F, Gonzalez-Maeso J, Sealfon SC (2005) Nucleic Acids Res 33:e161
Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S (2005) Science 307:538
Alivisatos AP, Gu W, Larabell C (2005) Annu Rev Biomed Eng 7:55
Gao X, Yang L, Petros JA, Marshall FF, Simons JW, Nie S (2005) Curr Opin Biotechnol 16:63
Lidke DS, Nagy P, Heintzmann R, Arndt-Jovin DJ, Post JN, Grecco HE, Jares-Erijman EA, Jovin TM (2004) Nat Biotechnol 22:198
Mattoussi H, Mauro JM, Goldman ER, Anderson GP, Sunder VC, Mikulec FV, Bawendi MG (2000) J Am Chem Soc 122:12142
Chan WCW, Nie S (1998) Science 281:2016
Gerion D, Pinaud F, Willimas SC, Parak WJ, Zanchet D, Weiss S, Alivisatos AP (2001) J Phys Chem B 105:8861
Bruchez M Jr, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Science 281:2013
Gao X, Cui Y, Levenson RM, Chung LWK, Nie S (2004) Nat Biotechnol 22:969
Clapp AR, Medintz IL, Mattoussi H (2006) ChemPhysChem 7:47
Sapsford KE, Berti L, Medintz IL (2006) Angew Chem Int Ed 45:4562
Smith AM, Duan H, Rhyner MN, Ruan G, Nie S (2006) Phys Chem Chem Phys 8:3895
Pellegrino T, Kudera S, Liedl T, Muñoz Javier A, Manna L, Parak WJ (2005) Small 1:48
Bäumle M, Stamou D, Segura JM, Hovius R, Vogel H (2004) Langmuir 20:3828
Zheng Y, Gao S, Ying JY (2007) Adv Mater 19:376
Medintz IL, Clapp AR, Mattoussi H, Goldman ER, Fisher B, Mauro JM (2003) Nat Mater 2:630
Uyeda HT, Medintz IL, Jaiswal JK, Simon SM, Mattoussi H (2005) J Am Chem Soc 127:3870
Willard DM, Carillo LL, Jung J, Orden AV (2001) Nano Lett 1:469
Wang S, Mamedova N, Kotov NA, Chen W (2002) Nano Lett 2:817
Patolsky F, Gill R, Weizmann Y, Mokari T, Banin U, Willner I (2003) J Am Chem Soc 125:13918
Moyzis RK, Buckingham JM, Cram LS, Dani M, Deaven LL, Jones MD, Meyne J, Ratcliff RL, Wu JR (1988) Proc Natl Acad Sci USA 85:6622
Bryan TM, Cech TR (1999) Curr Opin Cell Biol 11:318
Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, Coviello GM, Wright WE, Weinrich SL, Shay JW (1994) Science 266:2011
Shay JW, Bacchetti S (1997) Eur J Cancer 33:787
Medintz IL, Clapp AR, Mattoussi H, Goldman ER, Fisher B, Mauro JM (2003) Nat Mater 2:630
Goldman ER, Medintz IL, Whitley JL, Hayhurst A, Clapp AR, Uyeda HT, Deschamps JR, Lassman ME, Mattoussi H (2005) J Am Chem Soc 127:6744
Medintz IL, Clapp AR, Brunel FM, Tiefenbrunn T, Uyeda HT, Chang EL, Deschamps JR, Dawson PE, Mattoussi H (2006) Nat Mater 5:581
Shi L, De Paoli V, Rosenzweig N, Rosenzweig Z (2006) J Am Chem Soc 128:10378
Gill R, Freeman R, Xu J, Willner I, Winograd S, Shweky I, Banin U (2006) J Am Chem Soc 128:15376
Kim JH, Morikis D, Ozkan M (2004) Sens Actuators B 102:315
Kim JH, Chaudhary S, Ozkan M (2007) Nanotechnology 18:1
Gill R, Willner I, Shweky I, Banin U (2005) J Phys Chem B 109:23715
Hansen JA, Wang J, Kawde AN, Xiang Y, Gothelf KV, Collins G (2006) J Am Chem Soc 128:2228
Polsky R, Gill R, Willner I (2006) Anal Chem 78:2268
Pavlov V, Xiao Y, Shlyahovsky B, Willner I (2004) J Am Chem Soc 126:11768
Liu J, Lu Y (2006) Angew Chem Int Ed 45:90
Levy M, Cater SF, Ellington AD (2005) ChemBioChem 6:2163
Choi JH, Chen KH, Strano MS (2006) J Am Chem Soc 128:15584
Willner I, Patolsky F, Wasserman J (2001) Angew Chem Int Ed 40:1861
Gill R, Patolsky F, Katz E, Willner I (2005) Angew Chem Int Ed 44:4554
Freeman R, Gill R, Beissenhirtz M, Willner I (2007) Photochem Photobiol Sci 6:416
Katz E, Zayats M, Willner I, Lisdat F (2006) Chem Comm, p 1395
Pardo-Yissar V, Katz E, Wasserman J, Willner I (2003) J Am Chem Soc 125:622
Porath D, Cuniberty G, Di Felice R (2004) Top Curr Chem 237:183
De Pablo PJ, Moreno-Herrero F, Colchero J (2000) Phys Rev Lett 85:4992
Willner I (2002) Science 298:2407
Stoll C, Kudera S, Parak WJ, Lisdat F (2006) Small 2:741
Bakalova R, Ohba H, Zhelev Z, Ishikawa M, Baba Y (2004) Nat Biotechnol 22:1360
Author information
Authors and Affiliations
Corresponding author
Editor information
Rights and permissions
Copyright information
© 2007 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Zayats, M., Willner, I. (2007). Photoelectrochemical and Optical Applications of Semiconductor Quantum Dots for Bioanalysis. In: Renneberg, R., Lisdat, F. (eds) Biosensing for the 21st Century. Advances in Biochemical Engineering/Biotechnology, vol 109. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2007_077
Download citation
DOI: https://doi.org/10.1007/10_2007_077
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-75200-4
Online ISBN: 978-3-540-75201-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)