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Engineering of Cell Proliferation Via Myc Modulation

  • Chapter
Systems Biology

Part of the book series: Cell Engineering ((CEEN,volume 5))

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Abstract

The use of metabolic engineering for the enhancement and control of cell proliferation is a rapidly developing field in biotechnology. Great research interest has been directed towards the development of proliferation and apoptosis controlled cell lines with high cell density, regulated proliferation, apoptosis resistance, and easy adaptation into serum free cultures. These are some of the desirable characteristics for the cost effective production of biopharmaceuticals, mainly because genetically modified cell lines can afford greater efficiency and control. Some of the strategies employed by metabolic engineering for the management of cell proliferation include the control of external factors in the culture environment, suppression of growth inhibitors, and over-expression of important regulators of proliferation and apoptosis pathways, such as growth factors and genes. c-myc is such a prime candidate to achieve these objectives. In its role as a transcription factor Myc can regulate an extensive array of biological activities by modulating the expression of a large number of genes that in turn regulate multiple downstream events. More importantly, Myc can regulate cell proliferation and transform cells in such a manner as to consider the advantages of utilising its unique characteristics into the development of cell lines with major significance in animal cell culture biotechnology

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Authors and Affiliations

  1. Clinical Sciences Research Institute, Warwick Medical School, Walsgrave Hospital, Coventry, CV2 2DX, UK

    Vasiliki Ifandi

  2. Biomedical Research Institute, Dept. of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

    Vasiliki Ifandi

  3. School of Chemical and Bioprocess Engineering, and Centre for Synthesis and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland

    Mohamed Al-Rubeai

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  1. University College Dublin, Ireland

    Mohamed Al-Rubeai

  2. Swiss Federal Institute of Technology, ETH Hoenggeerberg HCI Institute of Chemical and Bioengineering ICB, Zurich, Switzerland

    Martin Fussenegger

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Ifandi, V., Al-Rubeai, M. (2007). Engineering of Cell Proliferation Via Myc Modulation. In: Al-Rubeai, M., Fussenegger, M. (eds) Systems Biology. Cell Engineering, vol 5. Springer, Dordrecht. https://doi.org/10.1007/1-4020-5252-9_5

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