Abstract
Four geographic strains of B. calyciflorus are investigated regarding their genetic similarity and ability to cross-mate. DNA sequence analysis of the mitochondrial cox1 gene (694 bp) and the nuclear ribosomal ITS region (735 bp) showed that the Florida and Georgia strains were very similar to each other (0.3% sequence divergence for the 1429 bp) and different from the Texas and Australia strains (∼7% and 9% sequence divergence for the 1429 bp, respectively). Consistent with this genetic relatedness, cross-copulation occurred only between the Florida and Georgia strains. Thus, B. calyciflorus is a complex of cryptic species. While the Florida, Texas and Australia strains were reproductively isolated from one another, most combinations of cross-strain mating tests showed intense and prolonged male circling behavior following male-female encounters. This suggests that precopulatory male circling and copulation are two separate behaviors that may be controlled by different female chemicals and male coronal receptors. In some cross-strain mating tests, females regularly retracted their corona when circled by a male, indicating that they can recognize ‘foreign’ males and actively interfere with copulation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Avise, J. C., 1994. Molecular Markers, Natural History and Evolution. Chapman & Hall Inc, New York NY.
Avise, J. C. & D. Walker, 1999. Species realities and numbers in sexual vertebrates: perspectives from an asexually transmitted genome. Proceedings of the National Academy of Sciences USA 96: 992–995.
Ciros-Pérez, J., A. Gómez & M. Serra, 2001. On the taxonomy of three sympatric sibling species of the Brachionus plicatilis (Rotifera) complex from Spain, with the description of B. ibericus n. sp. Journal of Plankton Research 23: 1311–1328.
Derry, A. M., P. D. N. Hebert & E. E. Prepas, 2003. Evolution of rotifers in saline and subsaline lakes: a molecular phylogenetics approach. Limnology and Oceanography 48: 675–685.
Folmer, O., M. Black, W. Hoeh, R. Lutz & R. Vrijenhoek, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit 1 from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294–299.
Gilbert, J. J., 1963. Contact chemoreception, mating behaviour, and sexual isolation in the rotifer genus Brachionus. Journal of Experimental Biology 40: 625–641.
Gilbert, J. J., 2002. Endogenous regulation of environmentally induced sexuality in a rotifer: a multigenerational parental effect induced by fertilisation. Freshwater Biology 47: 1633–1641.
Gilbert, J. J., 2003. Specificity of crowding response that induces sexuality in the rotifer Brachionus. Limnology and Oceanography 48: 1297–1303.
Gilbert, J. J., 2004. Population density, sexual reproduction and diapause in monogonont rotifers: new data for Brachionus and a review. Journal of Limnology 63(Suppl. 1): 32–36.
Gómez, A. & M. Serra, 1995. Behavioral reproductive isolation among sympatric strains of Brachionus plicatilis Müller 1786: insights into the status of this taxonomic species. Hydrobiologia 313/314: 111–119.
Gómez, A. & M. Serra, 1996. Mate choice in male Brachionus plicatilis rotifers. Functional Ecology 10: 681–687.
Gómez, A., M. Serra, G. R. Carvalho & D. H Lunt, 2002. Speciation in ancient cryptic species complexes: evidence from the molecular phylogeny of Brachionus plicatilis (Rotifera). Evolution 56: 1431–1444.
Koste, W. & R. J. Shiel, 1987. Rotifera from Australian inland waters II. Epiphanidae and Brachionidae (Rotifera: Monogononta). Invertebrate Taxonomy 1: 949–1021.
Kutikova, L. A. & C. H. Fernando, 1995. Brachionus calyciflorus Pallas (Rotatoria) in inland waters of tropical latitudes. Internationale Revue der gesamten Hydrobiologie 80: 429–441.
Lee, C. E., 2000. Global phylogeography of a cryptic copepod species complex and reproductive isolation between genetically proximate “populations”. Evolution 54: 2014–2027.
Lee, C. E. & B. W. Frost, 2002. Morphological stasis in the Eurytemora affinis species complex (Copepoda: Temoridae). Hydrobiologia 480: 111–128.
Moriyama, E. N. & J. R. Powell, 1996. Intraspecific nuclear DNA variation in Drosophilia. Molecular Biology and Evolution 13: 261–277.
Segers, H., 1995. Nomenclatural consequences of some recent studies of Brachionus plicatilis (Rotifera, Brachionidae). Hydrobiologia 313/314: 121–122.
Snell, T. W., 1998. Chemical ecology of rotifers. Hydrobiologia 387/388: 267–276.
Snell, T. W. & M. Childress, 1987. Aging and loss of fertility in male and female Brachionus plicatilis (Rotifera). International Journal of Invertebrate Reproduction and Development 12: 103–110.
Snell, T. W., M. J. Childress & B. C. Winkler, 1988. Characteristics of the mate recognition factor in the rotifer Brachionus plicatilis. Comparative Biochemistry and Physiology 89A: 481–485.
Snell, T. W. & F. H. Hoff, 1987. Fertilization and male fertility in the rotifer Brachionus plicatilis. Hydrobiologia 147: 329–334.
Snell, T. W., R. Rico-Martinez, L. N. Kelly & T. E. Battle, 1995. Identification of a sex pheromone from a rotifer. Marine Biology 123: 347–353.
Swofford, D. L., 2002. PAUP*. Phylogenetic analysis using parsimony (* and other methods), vers. 4.0. Sinauer Associates, Sunderland, MA.
Thompson, J. D., D. G. Higgings & T. J. Gibson., 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequencing weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4676–4680.
White T. J., J. Bruns, S. Lee & J. Taylor, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis, M., D. Gelfand, J. Sninsky & T. White (eds), PCR Protocols: A Guide to Methods and Applications.
Zar J. H., 1999. Biostatistical Analysis. Prentice Hall.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer
About this chapter
Cite this chapter
Gilbert, J.J., Walsh, E.J. (2005). Brachionus calyciflorus is a species complex: Mating behavior and genetic differentiation among four geographically isolated strains. In: Herzig, A., Gulati, R.D., Jersabek, C.D., May, L. (eds) Rotifera X. Developments in Hydrobiology, vol 181. Springer, Dordrecht. https://doi.org/10.1007/1-4020-4408-9_26
Download citation
DOI: https://doi.org/10.1007/1-4020-4408-9_26
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3493-0
Online ISBN: 978-1-4020-4408-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)