Abstract
We have identified acetylcholinesterase (AChE) receptors in six freshwater rotifers. Using β-bungarotoxin labelled with fluoresceinisothiocyanate (FITC), muscarinic and nicotinic receptors were found in Brachionus quadridentatus (females and males), Lecane luna, Lecane quadridentata, Plationus patulus, and Rotaria neptunia. Using α-bungarotoxin-FITC, nicotinic receptors were identified in B. quadridentatus, Lecane bulla, L. luna, L. quadridentata, P. patulus and R. Neptunia. Concentrations as low as 1.5 nM of β-bungarotoxin, and 5 nM of α-bungarotoxin identified receptors in the digestive tract. Higher concentrations of both toxins identified additional receptors associated with the lorica. A preliminary analysis of fluorescence intensity in L. quadridentata showed that response to α-bungarotoxin increases with age from newborn to 48-h old, but not in older individuals, thus suggesting an increase in binding sites, and possibly in number of nicotinic receptors, during the first 48-h of life. Our study extends the number of rotifer species in which AChE receptors have been reported.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Barrantes, G. E., A. T. Rogers, J. Lindstrom & S. Wonnacott, 1995. Alpha-Bungarotoxin binding sites in rat hippocampal and cortical cultures: initial characterization, co-localization with alpha 7 subunits and up-regulation by chronic nicotine treatment. Brain Research 672: 228–236.
Chiapinelli, V. A., J. B. Cohen & R. E. Zigmond, 1981. The effect of alpha-neurotoxins from the venoms of various snakes on transmission in autonomic ganglia. Brain Research 211: 107–126.
Horch, H. L. & P. B. Sargent, 1995. Perisynaptic surface distribution of multiple classes of nicotinic acetylcholine receptors on neurons in the chicken ciliary ganglion. Journal of Neuroscience 15: 7778–7795.
Nogrady, T., 1987. Histofluorescent labelling of catecholaminergic structures in rotifer (Aschelminthes) in whole animals. Histochemistry 87: 351–357.
Nogrady, T., 1988. Histofluorescent labelling of catecholaminergic structures in rotifer (Aschelminthes). II, Males of Brachionus plicatilis and structures from sectioned females. Histochemistry 89: 189–192.
Koste, W., 1978. Rotatoria, Die Rädertiere Mittleleuropas. Überordnung Monogononta. Begründet von Max Voigt. Gebruder Borntraeger, Berlin, Stuttgart, Textband 673 pp, Tafelband, 234 Tafeln, 673 pp.
Kotikova, E. A., 1995. Localization and neuroanatomy of catecholaminergic neurons in some rotifer species. Hydrobiologia 313/314: 123–127.
Kotikova, E. A., 1998. Catecholaminergic neurons in the brain of rotifers. Hydrobiologia 387/388: 135–140.
Nichols, H. W., 1973. Growth Media-Freshwater. In J. R. Stein (ed.), Handbook of phycological methods. Cambridge University Press: 7–24.
Nogrady, T. & M. Alai, 1983. Cholinergic neurotransmission in rotifers. Hydrobiologia 104: 149–153.
Nogrady, T. & J. Keshmirian, 1986a. Rotifer neuropharmacology I. Cholinergic drug effects on oviposition of Philodina acuticornis (Rotifera, Aschelminthes). Comparative Biochemistry & Physiology C 83: 335–338.
Nogrady, T. & J. Keshmirian, 1986b. Rotifer neuropharmacology. II Synergistic effects on local anesthetic activity in Brachionus calyciflorus (Rotifera, Aschelminthes). Comparative Biochemistry & Physiology C 83: 339–344.
Nogrady, T., R. L. Wallace & T. W. Snell, 1993. Rotifera 1. Biology, Ecology and Systematics. Guides to the Identification of the Microinvertebrates of the Continental Waters of the World Volume 4, SPB Academic Publishing, New York, 142 pp.
Ochillo, R. F., C. S. Tsai & M. H. Tsai, 1981. Mechanism of action of muscarine on the longitudinal muscle of the guinea-pig isolated ileum. British Journal of Pharmacology 72: 225–232.
Raineri, M., 1984. Histochemical investigations of Rotifera Bdelloidea. I. Localization of cholinesterase activity. Histochemical Journal 16: 601–616.
Rowan, E. G., 2001. What does beta-bungarotoxin do at the neuromuscular junction? Toxicon 39: 107–118.
Segers, H., 1995. Rotifera 2. The Lecanidae (Monogononta). Guides to the Identification of the Microinvertebrates of the Continental Waters of the World. Volume 6 SPB Academic Publishing, The Hague, The Netherlands, 226 pp.
Stemberger, R. S., 1979. A Guide to Rotifers of the Laurentian Great Lakes. Environmental Protection Agency, EPA-600/4-79-021, Cincinnati, Ohio, 186 pp.
Stiles, B. G., 1993. Acetylcholine receptor binding characteristics of snake and cone snail venom postsynaptic neurotoxins: further studies with a non-radioactive assay. Toxicon, 825-834.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer
About this chapter
Cite this chapter
Pineda-Rosas, A., Santos-Medrano, G., Zavala-Reynoso, M., Rico-Martínez, R. (2005). Identification of acetylcholinesterase receptors in Rotifera. In: Herzig, A., Gulati, R.D., Jersabek, C.D., May, L. (eds) Rotifera X. Developments in Hydrobiology, vol 181. Springer, Dordrecht. https://doi.org/10.1007/1-4020-4408-9_25
Download citation
DOI: https://doi.org/10.1007/1-4020-4408-9_25
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3493-0
Online ISBN: 978-1-4020-4408-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)