Abstract
Superoxide dismutases (SODs) promote a conversion of harmful reactive oxygen species (ROS) to relatively moderate forms, resulting in the extension of lifespan in the nematode Caenorhabditis elegans under caloric restriction. The lifespan of the rotifer Brachionus plicatilis is also markedly extended by caloric restriction. We, therefore, cloned cDNA encoding SOD activated with Mn (Mn SOD) from B. plicatilis and examined its expression pattern in rotifers raised with energy restricted diet. The full length deduced amino acid sequence of the rotifer Mn SOD showed 61% identity with the C. elegans ortholog. Four amino acid residues that are essential to the binding of this enzyme to Mn were conserved in the rotifer Mn SOD. Subsequently we examined the mRNA expression patterns of Mn SOD using highly sensitive quantitative real-time PCR for various rotifer populations that are likely to differ in their lifespans in experiments on calorie restricted diets. The accumulated mRNA levels of Mn SOD were found to increase in supposedly long-lived rotifers. These results suggest that Mn SOD is possibly related to the aging of B. plicatilis.
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References
Chu, C. C. & W. E. Paul, 1998. Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis. Molecular Immunology 35: 487–502.
DiSilvestre, D., S. R. Kleeberger, J. Johns & R. C. Levitt, 1995. Structure and DNA sequence of the mouse MnSOD gene. Mammalian Genome 6: 281–284.
Enesco, H. E., 1993. Rotifers in aging research: use of rotifers to test various theories of aging. Hydrobiologia 255/256: 59–70.
Finkel, T. & N. J. Holbrook, 2000. Oxidants, oxidative stress and the biology of ageing. Nature 408: 239–247.
Furuyama, T., T. Nakazawa, I. Nakano & N. Mori, 2000. Identification of the differential distribution patterns of mRNAs and consensus binding sequences for mouse DAF-16 homologues. Biochemical Journal 349: 629–634.
Guarente, L. & C. Kenyon, 2000. Genetic pathways that regulate ageing in model organisms. Nature 408: 255–262.
Honda, Y. & S. Honda, 1999. The daf-2 gene network for longevity regulates oxidative stress resistance and Mn-superoxide dismutase gene expression in Caenorhabditis elegans. FASEB Journal 13: 1385–1393.
Johnston, M., S. Andrews, R. Brinkman, J. Cooper, H. Ding, J. Dover, Z. Du, A. Favello, L. Fulton, S. Gattung, et al., 1994. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077–2082.
Kaneko, G., S. Kinoshita, T. Yoshinaga, K. Tsukamoto & S. Watabe, 2002. Changes in expression patterns of stress protein genes during population growth of the rotifer Brachionus plicatilis. Fisheries Science 68: 1317–1323.
Leblanc, C., A. Falciatore, M. Watanabe & C. Bowler, 1999. Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatom. Plant Molecular Biology 40: 1031–1044.
Marres, C. A., A. P. Van Loon, P. Oudshoorn, H. Van Steeg, L. A. Grivell & E. C. Slater, 1985. Sequence analysis of the nuclear gene coding for manganese superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron-sulphur protein. European Journal of Biochemistry 147: 153–161.
Parkes, T. L., A. J. Elia, D. Dickinson, A. J. Hilliker, J. P. Phillips & G. L. Boulianne, 1998. Extension of Drosophila lifespan by overexpression of human SOD1 in motorneurons. Nature Genetics 19: 171–174.
Piano, F., A. J. Schetter, M. Mangone, L. Stein & K. J. Kemphues, 2000. RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans. Current Biology 10: 1619–1622.
Verdone-Smith, C. & H. E. Enesco, 1982. The effect of temperature and of dietary restriction on lifespan and reproduction in the rotifer Asplanchna brightwelli. Experimental Gerontology 17: 255–262.
Wispe, J. R., J. C. Clark, M. S. Burhans, K. E. Kropp, T. R. Korfhagen & J. A. Whitsett, 1989. Synthesis and processing of the precursor for human manganese-superoxide dismutase. Biochimica et Biophysica Acta 994: 30–36.
Yoshinaga, T., A. Hagiwara & K. Tsukamoto, 1999. Effect of conditioned media on the asexual reproduction of the monogonont rotifer Brachionus plicatilis O. F. Müller. Hydrobiologia 412: 103–110.
Yoshinaga, T., A. Hagiwara & K. Tsukamoto, 2000. Effect of periodical starvation on the life history of Brachionus plicatilis O. F. Mü ller (Rotifera): a possible strategy for population stability. Journal of Experimental Marine Biology and Ecology 253: 253–260.
Yoshinaga, T., A. Hagiwara & K. Tsukamoto, 2003. Life history response and age-specific tolerance to starvation in Brachionus plicatilis O. F. Müller. Journal of Experimental Marine Biology and Ecology 287: 261–271.
Zelko, I. N., T. J. Mariani & R. J. Folz, 2002. Superoxide dismutase multigene family; a comparison of the Cu/Zn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radical Biology and Medicine 33: 337–349.
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Kaneko, G., Yoshinaga, T., Yanagawa, Y., Kinoshita, S., Tsukamoto, K., Watabe, S. (2005). Molecular characterization of Mn-superoxide dismutase and gene expression studies in dietary restricted Brachionus plicatilis rotifers. In: Herzig, A., Gulati, R.D., Jersabek, C.D., May, L. (eds) Rotifera X. Developments in Hydrobiology, vol 181. Springer, Dordrecht. https://doi.org/10.1007/1-4020-4408-9_10
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DOI: https://doi.org/10.1007/1-4020-4408-9_10
Publisher Name: Springer, Dordrecht
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