Abstract
Transfer of T-DNA from binary vectors to transgenic “hairy roots” rely on co-transformation of the T-DNA from the binary vector and the T-DNA from the Ri plasmid into the same plant cell. The frequency of co-transformation varies, so for some experiments an alternative method integrating the gene construct under study diretly into the Ri T-DNA segment may be useful.
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Stougaard J. (1995) Agrobacterium rhizogenes as a vector for transforming higher plants. Methods in Molecular Biology 49, 49–61.
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© 2005 Springer
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Radutoiu, S., Madsen, L.H., Madsen, E.B., Nielsen, A.M., Stougaard, J. (2005). Agrobacterium rhizogenes pRi TL-DNA integration system: a gene vector for Lotus japonicus transformation. In: Márquez, A.J. (eds) Lotus japonicus Handbook. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3735-X_28
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DOI: https://doi.org/10.1007/1-4020-3735-X_28
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3734-4
Online ISBN: 978-1-4020-3735-1
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