Abstract
The model legume Lotus japonicus can be transformed and regenerated efficiently with Agrobacterium tumefaciens or A. rhizogenes. However, it takes between 8 to 12 months to obtain seeds of transgenic plants. We therefore developed a rapid and efficient transformation protocol using A. rhizogenes to induce transgenic hairy roots that can be inoculated with Mesorhizobium loti 2 weeks after transformation. The first nodules emerge 8 to 10 days after inoculation, as on the roots of wild type Lotus plants and expression of plant genes involved in any step of nodulation can be completed within two months after the start of a transformation-nodulation experiment. A large number of seedlings can be tranformed in one experiment, allowing addressing of a number of variables in one single tranformationnodulation experiment.
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Díaz, C.L., Grønlund, M., Schlaman, H.R.M., Spaink, H.P. (2005). Induction of hairy roots for symbiotic gene expression studies. In: Márquez, A.J. (eds) Lotus japonicus Handbook. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3735-X_26
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DOI: https://doi.org/10.1007/1-4020-3735-X_26
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3734-4
Online ISBN: 978-1-4020-3735-1
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