Abstract
The following protocol provides a high throughput, low cost method of producing a superior DNA yield of high quality which is suitable for TILLING, map based cloning or any application which requires long term DNA storage. The protocol has been designed for DNA extraction from leaf material, preferably young leaf tissue should be used as this minimises samples being contaminated with polysaccharides and phenolics.
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References
Perry, JA, Wang, TL, Welham, TJ, Gardner, S, Pike, JM, Yoshida, S, and Parniske, M. (2003) A TILLING reverse genetics tool and a web-accessible collection of mutants of the legume Lotus japonicus. Plant Physiology 131, 866–871.
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© 2005 Springer
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Perry, J., Parniske, M. (2005). 96-Well DNA isolation method. In: Márquez, A.J. (eds) Lotus japonicus Handbook. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3735-X_11
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DOI: https://doi.org/10.1007/1-4020-3735-X_11
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3734-4
Online ISBN: 978-1-4020-3735-1
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