Abstract
This paper describes a microspectroscopic instrumentation suitable for performing absorption and fluorescence spectra of cellular compartments inside their functional environment. The system consists of an optical microscope, measuring diaphragms, diffraction gratings, and photodetectors. A personal computer acquires the signals from the photodetectors and handles the diffraction gratings. In this chapter the absorption and fluorescent spectra of Euglena gracilis photoreceptor and chloroplast are presented.
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Evangelista, V., Barsanti, L., Frassanito, A.M., Passarelli, V., Gualtieri, P. (2005). Wavelength as the Fourth Dimension in Light Microscopy. In: Evangelista, V., Barsanti, L., Passarelli, V., Gualtieri, P. (eds) From Cells to Proteins: Imaging Nature across Dimensions. NATO Security through Science Series. Springer, Dordrecht . https://doi.org/10.1007/1-4020-3616-7_15
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DOI: https://doi.org/10.1007/1-4020-3616-7_15
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3614-9
Online ISBN: 978-1-4020-3616-3
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