Abstract
Microbial parameters derived from the short-term Michaelis-Menten type model are tested and applied on the ecosystem study. Soil dried immediately after sampling and stored at 4°C was moistened to 60% water holding capacity and CO2 production was measured (GC) after 24 h (respiration response to water supply, VDS) and between the 5th and 6th day of incubation (basal respiration VBR). Then glucose was added into the soil and CO2 production was measured 16 to 24 h later (maximum respiration, VMAX). Substrate saturation kinetics of respiration was measured after addition of glucose in 6 different concentrations. Soil heterotrophic respiratory potential was expressed as VDS/VMAX ratio; biologically available C (ACBR) and potential flush of the biologically available C (ACDS/ACBR) was estimated using Michaelis-Menten type model. It is shown that the above parameters can get relevant results because they meet the basic assumptions: (i) Fitting of Michaelis-Menten type model is accurate (R2 = 0.956–0.994), (ii) microbial respiration is substrate limited in natural conditions, (iii) VMAX is relatively invariable for a wide range of C substrate concentrations and microbial populations are not growing. (iv) After moistening of the soil, extra C is released, the amount of which is characteristic for the given soil. Application of the short-term kinetic approach on the upper soil layer of various ecosystems (Western Canada transect, Central Siberia transect) revealed that except for climate texture, which is closely linked to other physico-chemical parameters of soil, is an important parameter controlling biological activity of soils. The soil profile (from 0 to 100 cm) study performed at two Russian locations (Fyodorovskoye, spruce forest on sedge bogs, and Zotino, pine forest on sands) demonstrates that the deep soil layers contain a high proportion of the biologically available C and are inhabited by microbial populations which are able to efficiently use the available C if the environmental conditions improve. The C accumulated in the peat soil profile is of higher quality than those accumulated in the coarse sand.
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Santruckova, H., Kurbatova, J.A., Shibistova, O.B., Smejkalova, M., Uhlirova, E. (2005). Short-Term Kinetics of Soil Microbial Respiration — A General Parameter Across Scales?. In: Binkley, D., Menyailo, O. (eds) Tree Species Effects on Soils: Implications for Global Change. NATO Science Series IV: Earth and Environmental Sciences, vol 55. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3447-4_13
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DOI: https://doi.org/10.1007/1-4020-3447-4_13
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