Skip to main content

The isolated Photosystem II reaction center: first attempts to directly measure the kinetics of primary charge separation

  • Chapter
Discoveries in Photosynthesis

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 20))

Abstract

Direct measurements of the intrinsic rate of primary charge separation in the isolated Photosystem II (PS II) reaction center complex had to await the availability of suitable, stabilized reaction center materials as well as sophisticated femtosecond transient absorption spectroscopic techniques. The events that led to the first direct measurements of the primary charge separation act in PS II and discussions of the results thereafter are chronicled in this brief historical review.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 349.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 449.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Brody SS (2002) Fluorescence lifetime, yield, energy transfer and spectrum in photosynthesis. Photosynth Res 73: 127–132

    Article  PubMed  CAS  Google Scholar 

  • Deisenhofer J, Epp O, Miki K, Huber R and Michel H (1985) Structure of the protein subunits in the photosynthetic reaction center of Rhodopseudomonas viridis at 3 Ã… resolution. Nature 318: 618–624

    Article  Google Scholar 

  • Dekker JP and van Grondelle R (2000) Primary charge separation in Photosystem II. Photosynth Res 63: 195–208

    Article  PubMed  CAS  Google Scholar 

  • Diner BA and Rappaport F (2002) Structure, dynamics, and energetics of the primary photochemistry of Photosystem II of oxygenic photosynthesis. Ann Rev Pl Biol 53: 551–580

    Article  CAS  Google Scholar 

  • Durrant JR, Hastings G, Joseph DM, Barber J, Porter G and Klug DR (1992) Subpicosecond equilibration of excitation energy in isolated Photosystem II reaction centers. Proc Natl Acad Sci USA 89: 11632–11636

    Article  PubMed  CAS  Google Scholar 

  • Govindjee and Krogmann D (2002) A list of personal perspectives with selected quotations, along with lists of tributes, historical notes, Nobel and Kettering awards related to photosynthesis. Photosynth Res 73: 11–20

    Article  CAS  Google Scholar 

  • Greenfield SR and Wasielewski MR (1995) Near-transform-limited visible and near-IR femtosecond pulses from optical parametric amplification using type-II beta-barium borate. Opt Lett 20: 1394–1396

    CAS  Google Scholar 

  • Greenfield SR and Wasielewski MR (1996) Excitation energy transfer and charge separation in the isolated Photosystem II reaction center. Photosynth Res 48: 83–97

    Article  CAS  Google Scholar 

  • Greenfield SR, Seibert M, Govindjee and Wasielewski MR (1996) Wavelength and intensity dependent primary photochemistry of isolated Photosystem II reaction centers at 5 °C Chem Physics 210: 279–295

    Article  CAS  Google Scholar 

  • Greenfield SR, Seibert M, Govindjee and Wasielewski MR (1997) Direct measurement of the effective rate constant for primary charge separation in isolated Photosystem II reaction centers. J Phys Chem B 101: 2251–2255

    Article  CAS  Google Scholar 

  • Greenfield SR, Seibert M and Wasielewski MR (1999) Time-resolve absorption changes of the pheophytin QX band in isolated Photosystem II reaction centers at 7 K: energy transfer and charge separation. J Phys Chem B 103: 8364–8374

    Article  CAS  Google Scholar 

  • Hastings G, Durrant JR, Barber J, Porter G and Klug DR (1992) Observation of pheophytin reduction in Photosystem two reaction centres using femtosecond transient absorption spectroscopy. Biochemistry 31: 7638–7647

    Article  PubMed  CAS  Google Scholar 

  • Jankowiak R, Tang D, Small GJ and Seibert M (1989) Transient and persistent hole burning of the reaction center of Photosystem II. J Phys Chem 93: 1649–1654

    Article  CAS  Google Scholar 

  • Jankowiak R, Rätsep M, Hayes J, Zabubovich V, Picorel R, Seibert M and Small GJ (2003) The primary charge separation rate at 5 K in isolated Photosystem II reaction centers containing 5 and 6 chlorophyll molecules. J Phys Chem (in press)

    Google Scholar 

  • McTavish H, Picorel R and Seibert (1989) Stabilization of isolated Photosystem II reaction center complex in the dark and in the light using polyethylene glycol and an oxygen-scrubbing system. Plant Physiol 89: 452–456

    PubMed  CAS  Google Scholar 

  • Metz JG, Bricker TM and Seibert M (1985) The azido[14C]atrazine photoaffinity technique labels a 34-kDa protein in Scenedesmus which function of the oxidizing sides of Photosystem II. FEBS Lett 185: 191–195

    Article  CAS  Google Scholar 

  • Metz JG, Pakrasi HR, Seibert M and Arntzen CJ (1986) Evidence for a dual function of the herbicide-binding D1 protein in Photosystem II. FEBS Lett 1205: 269–274

    Article  Google Scholar 

  • Michel H (1982) The three-dimensional crystals of a membrane protein complex. The photosynthetic reaction center from Rhodopseudomonas viridis. J Mol Biol 158: 567–572

    Article  PubMed  CAS  Google Scholar 

  • Müller MG, Hucke M, Reus M and Holzwarth AR (1996) Primary processes and structure of the Photosystem II reaction center. 4. Low-intensity femtosecond transient absorption spectra of D1–D2-cyt-b559 reaction centers. J Phys Chem 100: 9527–9536

    Article  Google Scholar 

  • Nanba O and Satoh K (1987) Isolation of a Photosystem II reaction center consisting of D1 and D2 polypeptides and cytochrome b-559. Proc Natl Acad Sci USA 84: 109–112

    Article  PubMed  CAS  Google Scholar 

  • Roelof TA, Gilbert A, Shuvalov VA and Holzwarth AR (1991) Picosecond fluorescence kinetics of the D1–D2-cyt-b-559 Photosystem II reaction center complex. Energy transfer and primary charge separation processes. Biochim Biophys Acta 1060: 237–244

    Google Scholar 

  • Sayre RT, Andersson B and Bogorad L (1986) The topology of a membrane protein: the orientation of the 32 kD QB-binding chloroplast thylakoid membrane protein. Cell 47: 601–608

    Article  PubMed  CAS  Google Scholar 

  • Schelvis JPM, van Noort PI, Aartsma, TJ and van Gorkom HJ (1994) Energy transfer, charge separation and pigment arrangement in the reaction center of Photosystem II. Biochim Biophys Acta 1184: 242–250

    Article  CAS  Google Scholar 

  • Seibert M, Picorel R, Rubin AB and Connolly JS (1988) Spectral, photophysical and stability properties of isolated Photosystem II reaction center. Plant Physiol 87: 303–306

    Article  PubMed  CAS  Google Scholar 

  • Trebst A (1986) The topology of the plastoquinone and herbicide binding peptides of Photosystem II in the thylakoid membrane. Z Naturforsch 41c: 240–245

    Google Scholar 

  • Wang J, Gosztola D, Ruffle SV, Hemann C, Seibert M, Wasielewski, MR, Hille R, Gustafson TL and Sayre RT (2002) Functional asymmetry of Photosystem II D1 and D2 peripheral chlorophyll mutants of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 99: 4091–4096

    Article  PubMed  CAS  Google Scholar 

  • Wasielewski MR, Fenton JM and Govindjee (1987) The rate of formation of P700 [+]-Ao[−] in Photosystem I particles from spinach as measured by picosecond transient absorption spectroscopy. Photosynth Res 12: 181–190

    Article  CAS  Google Scholar 

  • Wasielewski MR, Johnson DG, Seibert M and Govindjee (1989a) Determination of the primary charge separation rate in isolated Photosystem II reaction centers with 500fs-time resolution. Proc Natl Acad Sci USA 86: 524–528

    Article  PubMed  CAS  Google Scholar 

  • Wasielewski MR, Johnson DG, Preston C, Govindjee and Seibert M (1989b) Determination of the primary charge separation rate in Photosystem II reaction centers at 15 K. Photosynth Res 22: 89–99

    Article  CAS  Google Scholar 

  • Wiederrecht GP, Seibert M, Govindjee and Wasielewski MR (1994) Femtosecond photodichrosim studies of isolated Photosystem II reaction centers. Proc Natl Acad Sci USA 91: 8999–9003

    Article  PubMed  CAS  Google Scholar 

  • Zouni A, Witt HT, Kern J, Fromme P, Krauss N and Orth P (2001) Crystal structure of Photosystem II from Synechococcus elongatus at 3.8Ã… resolution. Nature 409: 739–743

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Springer

About this chapter

Cite this chapter

Seibert, M., Wasielewski, M.R. (2005). The isolated Photosystem II reaction center: first attempts to directly measure the kinetics of primary charge separation. In: Govindjee, Beatty, J.T., Gest, H., Allen, J.F. (eds) Discoveries in Photosynthesis. Advances in Photosynthesis and Respiration, vol 20. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3324-9_26

Download citation

Publish with us

Policies and ethics