Abstract
Escherichia coli isolates from 131 raw chicken meat samples were tested for susceptibility to 12 antibiotics. Plasmids were isolated from many samples and their DNA molecular weight calculated. An 81.7% plasmid occurrence rate was observed among the isolates, ranging from 0 to 8 in number and with sizes from 1.2 to 118.6 MDa. Plasmids were detected in 93.8% of E. coli isolates resistant to all 12 antibiotics, and in 90.5% of E. coli isolates resistant to 11. Three (2.8%) isolates harboured 8 plasmids and were resistant to all 12 antibiotics. Antibiotic resistant genes in bacteria are usually carried in extrachromosomal DNA and it is postulated that E. coli with a high number of plasmids possesses wider resistance to antibiotics.
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References
Bauer, A.W., Kirby, W.M.M. & Sheris, J.S. 1966. Antibiotic susceptibility testing by a standard single disc method. American Journal of Clinical Pathology, 45: 493–496.
Bichler, R.R., de Vries, J. & Rombouts, F.M. 1994. Plasmid diversity in Salmonella enteritidis of animal, poultry and human origin. Journal of Food Protection, 57: 307–311.
Geornaras, I., Hastings, J.H. & von Holy, A. 2001. Genotypic analysis of Escherichia coli strains from poultry carcasses and their susceptibilities to antimicrobial agents. Applied and Environmental Microbiology, 67: 1940–1944.
González, E.A. & Blanco, J. 1985. Relation between antibiotic resistance and number of plasmids in enterotoxigenic and non-enterotoxigenic Escherichia coli strains. Medical Microbiology and Immunology, 174: 257–265.
Macrina, F.L., Kopecko, D.J., Jones, K.K., Ayers, D.J. & Mc Gowen, S.M. 1978. A multiple plasmid-containing Escherichia coli strain: Convenient source of reference plasmid molecules. Plasmid, 1: 417.
Mahony, D.E., Ahmed, R. & Jackson, S.G. 1992. Multiple typing techniques applied to a Clostridium perfringens food poisoning outbreak. Journal of Applied Bacteriology, 72: 309–314.
Maniatis, J., Fritsch, E.F. & Sambrook, J. 1989. Molecular Cloning. A Laboratory Manual. 2nd edition. USA: Cold Spring Harbor-Laboratory Press.
Meyers, J.A., Sanchez, D., Elwell, L.P. & Falkow, S. 1976. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology, 127: 1529–1537. [See also correction in ibid., 129: 1171.]
O’Brien, T.F., Di Giorgio, J., Parsonnet, K.C., Kass, E.H. & Hopkins, J.D. 1993. Plasmid diversity in Escherichia coli isolated from processed poultry and poultry processors. Veterinary Microbiology, 35: 243–255.
Platt, D.J., Sommerville, J.S., Kraft, C.A. & Timbury, M.C. 1984. Antimicrobial resistance and the ecology of Escherichia coli plasmids. Journal of Hygiene (Cambridge), 93: 181–188.
Samuel, L. 1999. Molecular characterization of Escherichia coli isolated from raw milk and village chicken and broiler litter. MSc Thesis. Faculty of Food Science and Biotechnology, Universiti Putra Malaysia, Malaysia.
Selanders, J.R. & Levin, B.R. 1980. Genetic diversity and structure in Escherichia coli populations. Science, 210: 545–554.
Son, R., Razali, H., Syamir, A., Zainuri, A., Rusul, G. & Sahila, A.M. 1997a. Multiple antibiotic resistance (MAR) index and plasmids in Escherichia coli. Jurnal Veterinar Malaysia, 9: 23–25.
Son, R., Rozila, A., Rusul, G., Zainuri, E., Raha, A.R. & Sahilah, A.M. 1997c. Antibiotic resistance and plasmid profiles of Escherichia coli in raw milk and beef. Malaysian Applied Biology, 26: 77–81.
Son, R., Sahila, A.M., Gulum, R., Rozila, A. & Samuel, L. 1998a. Beef as a source of multi-resistant Escherichia coli O157: H7 harbouring transferable plasmid and resistance phenotype. Journal of Bioscience, 9: 20–27.
Son, R., Shahilah, A.M., Gulam, R., Zainori, A., Tadaaki, M., Norio, A., Yung, B.K., Jun, O. & Mitsuaki, N. 1998b. Detection of Escherichia coli O157:H7 in the beef marketed in Malaysia. Applied and Environmental Microbiology, 64: 1153–1156.
Son, R., Zainuri, A., Rusul, G., Karim, M.I.A., Ali, A.M. & Harikishna, K. 1997b. Survival of genetically engineered Escherichia coli in river water. Tropical Biomedicine, 14: 19–26.
Tacket, C.O. 1989. Molecular epidemiology of Salmonella. Epidemiologic Reviews, 11: 99–108.
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Myaing, T.T., Saleha, A., Arifah, A., Raha, A. (2005). Antibiotic Resistance and Plasmid Carriage among Escherichia Coli Isolates From Chicken Meat in Malaysia. In: Makkar, H.P., Viljoen, G.J. (eds) Applications of Gene-Based Technologies for Improving Animal Production and Health in Developing Countries. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3312-5_37
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DOI: https://doi.org/10.1007/1-4020-3312-5_37
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3311-7
Online ISBN: 978-1-4020-3312-4
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