Abstract
Embryogenic cultures of Norway spruce (Picea abies) were cultivated either on solidified media, in liquid media, or on polypropylene membrane rafts (LifeRaft). The cultivation on rafts was found to be the most successful way: the number of developed somatic embryos increased, synchronization of the development was enhanced, and the time necessary for embryo development and maturation was shortened. It was shown that the process could be further improved by insertion of a pre-maturation phase on PGR-free medium between proliferation and maturation steps. Germination frequency remained unchanged. PEG 4000 added to the maturation medium increased the number of developed somatic embryos. PEG in lower concentration (1.87 % (w/v)) still had the significant beneficial effects on embryo numbers and development, but a decrease of germination frequency or increased aberrations of developing root and shoot were not found. On the other hand, PEG in the concentration 5 and 7.5 % (w/v) had a negative effect on the germination of somatic embryos and, in sensitive cell line, 7.5 % (w/v) PEG decreased somatic embryo yield.
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References
Bozhkov PV, Filonova LH & von Arnold S (2002), A key developmental switch during Norway spruce somatic embryogenesis is induced by withdrawal of growth regulators and is associated with cell death and extracellular acidification. Biotech. Bioeng. 77: 658–667
Bozhkov PV & von Arnold S (1998) Polyethylene glycol promotes maturation but inhibits further development of Picea abies somatic embryos. Physiol. Plant. 104: 211–224
Find JI (1997) Changes in endogenous ABA levels in developing somatic embryos of Norway spruce (Picea abies (L.) Karst) in relation to maturation medium, desiccation and germination Plant Sci. 128: 75–83
Gorbatenko & Hakman I (2001) Desiccation-tolerant somatic embryos of Norway spruce (Picea abies) can be produced in liquid cultures and regenerated into plantlets. Int. J. Plant Sci. 162: 1211–1218
Gupta PK & Durzan DJ (1986) Plantlet regeneration via somatic embryogenesis from subcultured callus of mature embryos of Picea abies (Norway spruce). In Vitro Cell. Dev. Biol. — Plant 22: 685–688
Luckett DJ, Venkatanagappa S, Darvey NL & Smithard RA (1991) Anther culture of Australian wheat germplasm using modified C-17 medium and membrane rafts. Aust. J. Plant Physiol. 18: 357–367
Paek KY, Hahn EJ & Son SH (2001) Application of bioreactors for large-scale micropropagation systems of plants. In Vitro Cell. Dev. Biol. — Plant 37: 149–157
Paques M, Bercetche J & Dumas E (1992) Liquid media to improve and reduce the cost of in vitro conifer propagation. Acta Hortic. 319: 95–100
Paques M, Bercetche J & Palada M (1995) Prospects and limits of somatic embryogenesis of Picea abies. In: Jain SM, Gupta PK & Newton RJ (eds) Somatic Embryogenesis in Woody Plants: History, Molecular and Biochemical Aspects, and Applications (pp. 399–414), Kluwer Academic Publishers, Dordrecht
Tautorus TE & Dunstan DI (1995), Scale-up of embryogenic plant suspension cultures in bioreactors. In: Jain SM, Gupta PK & Newton RJ (eds) Somatic Embryogenesis in Woody Plants: History, Molecular and Biochemical Aspects, and Applications (pp. 265–292), Kluwer Academic Publishers, Dordrecht
Vágner M, Kumstýřová L, Opatrná J & Vondráková Z (2001) Norway spruce somatic embryogenesis: Bottlenecks of the method. (pp. 28–29) Proc. COST 843, WG 2, Thessaloniki, September 22.-25.2001
Vágner M, Vondráková Z, Strnadová Z, Eder J & Macháčková I (1998) Endogenous levels of plant growth hormones during early stages of somatic embryogenesis of Picea abies. Adv. Hort. Sci. 12: 11–18
Vágner M, Vondráková Z, Kumstýřová L, Opatrná J, Cvikrová M, Eder J, Vaněk T & Macháčková I (2000) Norway spruce somatic embryogenesis. (pp. 15–16) Proc. COST 843, WG 2 Meeting, July 2000, Tampere, Finland
Watad AA, Kochba M, Nissim A & Gaba V (1995) Improvement of Aconitum napelus micro-propagation by liquid culture on floating membrane rafts. Plant Cell Rep. 14: 345–348
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Vágner, M., Vondráková, Z., Fischerová, L., Opatrná, J. (2005). Norway spruce somatic embryogenesis: membrane rafts as a compromise between liquid and solidified media. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_21
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DOI: https://doi.org/10.1007/1-4020-3200-5_21
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3199-1
Online ISBN: 978-1-4020-3200-4
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