Abstract
The effect of physical and chemical environmental parameters on growth and differentiation of suspension cultures of the moss Physcomitrella patens in bioreactors was investigated. By supplementation of the aeration gas with 2 % (v/v) CO2 as well as by continuous illumination, growth of this photoautotrophic growing batch culture was markedly enhanced, resulting in a doubling time of 1.2 d. The growth rate of semi continuously-growing bioreactor cultures was not affected by controlling the pH of the culture medium with set points at 4.5 or 7.0. However, growth of the culture at pH 7.0 resulted in increased caulonema development, thus showing a distinct effect on moss differentiation. The impact on research and plant biotechnological applications of the potential to control moss growth and differentiation by environmental parameters is discussed.
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References
Bhatla SC, Kiessling J & Reski R (2002) Observation of polarity induction by cytochemical localization of phenylalkylamine-binding receptors in regenerating protoplasts of the moss Physcomitrella patens. Protoplasma 219: 99–105
Egener T, Granado J, Guitton MC, Hohe A, Holtorf H, Lucht JM, Rensing S, Schlink K, Schulte J, Schween G, Zimmermann S, Duwenig E, Rak B & Reski R (2002) High frequency of phenotypic deviations in Physcomitrella patens plants transformed with a gene-disruption library. BMC Plant Biology 2: 6
Hohe A & Reski R (2002) Optimisation of a bioreactor culture of the moss Physcomitrella patens for mass production of protoplasts. Plant Sci. 163: 69–74
Hohe A, Decker EL, Gorr G, Schween G & Reski R (2002) Tight control of growth and cell differentiation in photoautotrophically growing moss (Physcomitrella patens) bioreactor cultures. Plant Cell Rep. 20: 1135–1140
Hohe A, Schween G & Reski R (2001) Establishment of a semicontinuous bioreactor culture of Physcomitrella patens for mass production of protoplasts. Acta Hort. 560: 425–428
Minocha SC (1987) pH of the medium and the growth of cells in culture. In: Bonga JM & Durzan DJ (eds) Cell and Tissue Culture in Forestry Vol. 1 (pp. 125–141). Martinus Nijhoff Publishers, Dordrecht, Boston, Lancaster
Nishiyama T, Hiwatashi Y, Sakakibara K, Kato M & Hasebe M (2000) Tagged mutagenesis and gene-trap in the moss, Physcomitrella patens by shuttle mutagenesis. DNA Res. 7: 9–17
Reski R & Abel WO (1985) Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isopentenyladenine. Planta 165: 354–358
Reski R (1998a) Development, genetics and molecular biology of mosses. Bot. Acta 111: 1–15
Reski R (1998b) Physcomitrella and Arabidopsis: The David and Goliath of reverse genetics. Trends Plant Sci. 3: 209–210
Reski R (1999) Molecular genetics of Physcomitrella. Planta 208: 301–309
Reutter K & Reski R (1996) Production of a heterologous protein in bioreactor cultures of fully differentiated moss plants. Plant Tiss. Cult. Biotechnol. 2: 142–147
Reutter K, Atzorn R, Hadeler B, Schmülling T & Reski R (1998) Expression of the bacterial ipt gene in Physcomitrella rescues mutations in budding and plastid division. Planta 206: 196–203
Schaefer DG (2001) Gene targeting in Physcomitrella patens. Curr. Opin. Plant Biol. 4: 143–150
Scragg AH (1995) The problems associated with high biomass levels in plant cell suspensions. Plant Cell Tiss. Org. Cult. 43: 163–170
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Hohe, A., Reski, R. (2005). Control of growth and differentiation of bioreactor cultures of Physcomitrella by environmental parameters. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_10
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DOI: https://doi.org/10.1007/1-4020-3200-5_10
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3199-1
Online ISBN: 978-1-4020-3200-4
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