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Abstract

Immunoassays for the detection of the antigens of microorganisms remain important tools for the diagnosis and management of infectious diseases. Great strides have been made since the introduction of the early precipitation and agglutination assays in increasing the sensitivity, specificity, standardization, and automation of antigen tests (Hage, 1999; Carpenter, 2002; Constantine and Lana, 2003; Peruski and Peruski, 2003). Antigen tests have long been used to detect infectious agents that are difficult, slow, or hazardous to culture. However, antigen detection methods are especially useful for rapid diagnosis, whether in the clinic, emergency department, doctor’s office, or the central laboratory. Recently, simple one-step assays have been introduced that can provide results in 15 min with dramatic benefits to physician decision-making.

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References

  • Barenfanger, J., Drake, C., Leon, N., Mueller, T., & Troutt T. (2000). Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study. J Clin Microbiol, 38, 2824–2828.

    PubMed  CAS  Google Scholar 

  • Barletta, J.M., Edelman, D.C., & Constantine, N.T. (2004). Lowering the detection limits of HIV-1 viral load using real-time immuno-PCR for HIV-1 p24 antigen. Am J Clin Pathol, 122, 20–27.

    Article  PubMed  CAS  Google Scholar 

  • Bisno, A.L., Gerber, M.A.,Gwaltney, J.M. Jr, Kaplan, E.L., & Schwartz, R.H. (2002). Infectious Diseases Society of America. Practice guidelines for the diagnosis and management of group A streptococcal pharyngitis. Clin Infect Dis, 35, 113–125.

    Article  PubMed  Google Scholar 

  • Boivin, G., Hardy, I., & Kress, A. (2001). Evaluation of a rapid optical immunoassay for influenza viruses (FLU OIA test) in comparison with cell culture and reverse transcription- PCR. J Clin Microbiol, 39, 730–732.

    Article  PubMed  CAS  Google Scholar 

  • Bonner, A.B., Monroe, K. W., Talley, L.I., Klasner, A.E., & Kimberlin, D. W. (2003). Impact of the rapid diagnosis of influenza on physician decision-making and patient management in the pediatric emergency department: results of a randomized, prospective, controlled trail. Pediatrics, 112, 363–367.

    Article  PubMed  Google Scholar 

  • Carpenter, A.B. (2002). Antibody-based methods. In: Rose, N.R., Hamilton, R.G., & Detrick, B., eds. Manual of Clinical Laboratory Immunology, ASM Press, Washington, DC, pp. 6–25.

    Google Scholar 

  • CDC(Centers for Disease Control and Prevention), National Center for Infectious Diseases, Division of Parasitic Diseases (2004). Diagnostic Procedures for Stool Specimens: Detection of Parasite Antigens. Available at http://www.dpd.cdc.gov/dpdx/HTML/DiagnosticProcedures.htm.

    Google Scholar 

  • Chandrasena, T.G., Premaratna, R., Abeyewickrema, W., & de Silva, N.R. (2002). Evaluation of the ICT whole-blood antigen card test to detect infection due to Wuchereria bancrofti in Sri Lanka. Trans R Soc Trop Med Hyg, 96, 60–63.

    Article  PubMed  CAS  Google Scholar 

  • Constantine, N.T., & Lana, D.P. (2003). Immunoassays for the diagnosis of infectious diseases. In: Murray, P.R., Baron, E.J., Jorgenese, J.H., Pfaller, M.A., & Yolken, R.H., eds. Manual of Clinical Microbiology. Washington, DC, ASM Press, pp. 218–233.

    Google Scholar 

  • Cruciani, M., Marcati, P., Malena, M., Bosco, O., Serpelloni, G., & Mengoli, C. (2002). Meta-analysis of diagnostic procedures for Pneumocystis carinii pneumonia in HIV-1- infected patients. Eur Respir J, 20, 982–989.

    Article  PubMed  CAS  Google Scholar 

  • Garcia, L.S. (2001). Antibody and antigen detection in parasitic infections. In: Garcia, L.S., ed. Diagnostic Medical Parasitology,4th ed. ASM Press, Washington, DC, pp. 581–600.

    Google Scholar 

  • Gerna, G., Revello, M.G., Percivalle, E., & Morini, F. (1992). Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. J Clin Microbiol, 30, 1232–1237.

    PubMed  CAS  Google Scholar 

  • Hage, D.S. (1999). Immunoassays. Anal Chem, 71, 294R-304R.

    Article  PubMed  CAS  Google Scholar 

  • Hamilton, M.S., Abel, D.M., Ballam, Y.J., Otto, M.K., Nickell, A.F., Pence, L.M., Appleman, J.R., Shimasaki, C.D., & Achyuthan, K.E. (2002). Clinical evaluation of the ZstatFlu-II test: a chemiluminescent rapid diagnostic test for influenza virus. J Clin Microbiol, 40, 2331–2334.

    Article  PubMed  CAS  Google Scholar 

  • Kiska, D.L., Jones, M.C., Mangum, M.E., Orkiszewski, D., & Gilligan, P.H. (1995). Quality assurance study of bacterial antigen testing of cerebrospinal fluid. J Clin Microbiol, 33, 1141–1144.

    PubMed  CAS  Google Scholar 

  • Landry, M.L., & Ferguson, D. (2000a). 2-hour cytomegalovirus pp65 antigenemia assay for rapid quantitation of cytomegalovirus in blood samples. J Clin Microbiol, 38, 427–428.

    CAS  Google Scholar 

  • Landry, M.L., & Ferguson, D. (2000b). SimulFluor Respiratory Screen for rapid detection of multiple respiratory viruses in clinical specimens by immunofluorescence staining. J Clin Microbiol, 38, 708–711.

    CAS  Google Scholar 

  • Mahony, J.B., Coombes, B.K., & Chernesky, M.A. (2003). Chlamydia and Chlamydophilia. In: Murray. P., Barron, E.J., Jorgensen, J.H., Pfaller, M.A., & Yolken, R.H., eds. Manual of Clinical Microbiology,8th ed. ASM Press, Washington, DC, pp. 991–1004.

    Google Scholar 

  • Perfect, J.R., & Casadevall, A. (2002). Cryptococcosis. Infect Dis Clin North Am, 16, 837–874.

    Article  PubMed  Google Scholar 

  • Peruski, A.H., & Peruski, L.F. Jr. (2003). Immunological methods for detection and identification of infectious disease and biological warfare agents. Clin Diagn Lab Immunol, 10, 506–513.

    Article  PubMed  CAS  Google Scholar 

  • Roson, B., Fernandez-Sabe, N., Carratala, J., Verdaguer, R., Dorca, J., Manresa, F., & Gudiol, F. (2004). Contribution of a urinary antigen assay (Binax NOW) to the early diagnosis of pneumococcal pneumonia. Clin Infect Dis, 38, 222–226.

    Article  PubMed  Google Scholar 

  • Scicchitano, L.M., Shetterly, B., & Bourbeau, P.P. (1999). Evaluation of light diagnostics SimulFluor HSV/VZV immunofluorescence assay. Diagn Microbiol Infect Dis, 35, 205–208.

    Article  PubMed  CAS  Google Scholar 

  • Slinger, R., Milk, R., Gaboury, I., & Diaz-Mitoma, F. (2004). Evaluation of the QuickLab RSV test, a new rapid lateral-flow immunoassay for detection of respiratory syncytial virus antigen. J Clin Microbiol, 42, 3731–3733.

    Article  PubMed  CAS  Google Scholar 

  • Smith, M.D., Derrington, P., Evans, R., Creek, M., Morris, R., Dance, D.A., & Cartwright, K. (2003). Rapid diagnosis of bacteremic pneumococcal infections in adults by using the Binax NOW Streptococcus pneumoniae urinary antigen test: a prospective, controlled clinical evaluation. J Clin Microbiol, 41, 2810–2813.

    Article  PubMed  CAS  Google Scholar 

  • Storch, G.A. (2003). Rapid diagnostic tests for influenza. Curr Opin Pediatr, 15, 77–84.

    Article  PubMed  Google Scholar 

  • Thomas, C.F. Jr., Limper, A.H. (2004). Pneumocystis pneumonia. N Engl J Med, 350, 2487–2498.

    Article  PubMed  CAS  Google Scholar 

  • Thompson, R.B., & Miller, J.M. (2003). Specimen collection, transport, and processing: bacteriology. In: Murray, P., Barron, E.J., Jorgensen, J.H., Pfaller, M.A., & Yolken, R.H., eds. Manual of Clinical Microbiology,8th ed. ASMP ress, Washington, DC, pp. 286–330.

    Google Scholar 

  • Versalovic, J. (2003). Helicobacter pylori. Pathology and diagnostic strategies. Am J Clin Pathol, 119, 403–412.

    Article  PubMed  Google Scholar 

  • Waterer, G. W., Baselski, V.S., & Wunderink, R.G. (2001). Legionella and community acquired pneumonia: a review of current diagnostic tests from a clinician’s viewpoint. Am J Med, 110, 41–48.

    Article  PubMed  CAS  Google Scholar 

  • Wilhelmi, I., Colomina, J., Martin-Rodrigo, D., Roman, E., & Sanchez-Fauquier, A. (2001). New immunochromatographic method for rapid detection of rotaviruses in stool samples compared with standard enzyme immunoassay and latex agglutination techniques. Eur J Clin Microbiol Infect Dis, 20, 741–743.

    Article  PubMed  CAS  Google Scholar 

  • Wilkins, T.D., & Lyerly, D.M. (2003). Clostridium difficile testing: after 20 years, still challenging. J Clin Microbiol, 41, 531–534.

    Article  PubMed  Google Scholar 

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Campbell, S., Landry, M.L. (2006). Rapid Antigen Tests. In: Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/0-387-32892-0_3

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