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Molecular Strain Typing Using Repetitive Sequence–Based PCR

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Advanced Techniques in Diagnostic Microbiology

Abstract

Microbial strain typing is increasingly important in routine clinical microbiology laboratories as a method to track hospital-acquired infections. Although many methods can be used, this chapter focuses on one particular method used for typing of bacteria and fungi: repetitive sequence–based PCR (rep-PCR). A number of valuable reviews are available for more in-depth discussion of other technologies (Olive and Bean, 1999; Soll, 2000; Zaidi et al., 2003). The technology behind rep-PCR will be introduced with a review of traditional, manual rep-PCR. The latest advances will be highlighted with a discussion of the DiversiLab System, Bacterial Borcodes, Inc., Athens, GA, which is an automated rep-PCR technology. Additionally, we describe a comparison of the technologies, current applications in clinical microbiology laboratories, and future potential of rep-PCR as a routine clinical test.

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Frye, S.R., Healy, M. (2006). Molecular Strain Typing Using Repetitive Sequence–Based PCR. In: Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/0-387-32892-0_26

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