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Rapid Screening and Identification of Methicillin-Resistant Staphylococcus aureus

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Advanced Techniques in Diagnostic Microbiology
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Abstract

Staphylococcus aureus is a major pathogen responsible for both nosocomial and community-acquired infections. Although the first S. aureus isolates displaying resistance to methicillin (MRSA) were reported in the early 1960s (Barber, 1961), endemic strains of MRSA carrying multiple resistance determinants did not become a worldwide nosocomial problem until the early 1980s (Hryniewicz, 1999). The presence of MRSA in an institution is paralleled by an increased rate of bacteremia or other severe MRSA infections (Harbarth et al., 2000). MRSA-related bacteremia carries a threefold attributable cost and a threefold excess length of hospital stay when compared with methicillin-susceptible S. aureus (MSSA) bacteremia (Abramson and Sexton, 1999).

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References

  • Abramson, M. A., & Sexton, D. J. (1999). Nosocomial methicillin-resistant and methicillinsusceptible Staphylococcus aureus primary bacteremia: at what costs? Infect Control Hosp Epidemiol, 20, 408–411.

    Article  CAS  Google Scholar 

  • Aucken, H. M., Ganner, M., Murchan, S., Cookson, B. D., & Johnson, A. P. (2002). A new UK strain of epidemic methicillin-resistant Staphylococcus aureus (EMRSA-17) resistant to multiple antibiotics. J Antimicrob Chemother, 50, 171–175.

    Article  PubMed  CAS  Google Scholar 

  • Baba, T., Takeuchi, F., Kuroda, M., Yuzawa, H., Aoki, K., Oguchi, A., Nagai, Y., Iwama, N., Asano, K., Naimi, T., Kuroda, H., Cui, L., Yamamoto, K., & Hiramatsu, K. (2002). Genome and virulence determinants of high virulence community-acquired MRSA. Lancet, 359, 1819–1827.

    Article  PubMed  CAS  Google Scholar 

  • Barber, M. (1961). Methicillin-resistant staphylococci. J Clin Pathol, 14, 385–393.

    PubMed  CAS  Google Scholar 

  • Becker, A., Forster, D. H., & Kniehl, E. (2002). Oxacillin resistance screening agar base for detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol, 40, 4400–4401.

    Article  PubMed  CAS  Google Scholar 

  • Berger-Bachi, B., Barberis-Maino, L., Strassle, A., & Kayser, F. H. (1989). FemA, a hostmediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization. Mol Gen Genet, 219, 263–269.

    Article  PubMed  CAS  Google Scholar 

  • Berglund, C., Molling, P., Sjoberg, L., & Soderquist, B. (2005). Predominance of staphylococcal cassette chromosome mec(SCCmec) type IV among methicillin-resistant Staphylococcus aureus (MRSA) in a Swedish county and presence of unknown SCCmec types with Panton-Valentine leukocidin genes. Clin Microbiol Infect, 11, 447–456.

    Article  PubMed  CAS  Google Scholar 

  • Brancart, F., Rodriguez-Villalobos, H., Fonteyne, P. A., Peres-Bota, D., & Liesnard, C. (2005). Quantitative TaqMan PCR for detection of Pneumocystis jiroveci. J Microbiol Methods, 61, 381–387.

    Article  PubMed  CAS  Google Scholar 

  • BSAC. (1998). Revised guidelines for the control of methicillin-resistant Staphylococcus aureus infection in hospitals. British Society for Antimicrobial Chemotherapy, Hospital Infection Society and the Infection Control Nurses Association. J Hosp Infect, 39, 253–290.

    Google Scholar 

  • Carleton, H. A., Diep, B. A., Charlebois, E. D., Sensabaugh, G. F., & Perdreau-Remington, F. (2004). Community-adapted methicillin-resistant Staphylococcus aureus (MRSA): population dynamics of an expanding community reservoir of MRSA. J Infect Dis, 190, 1730–1738.

    Article  PubMed  CAS  Google Scholar 

  • Cavassini, M., Wenger, A., Jaton, K., Blanc, D. S., & Bille, J. (1999). Evaluation of MRSA-Screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus. J. Clin. Microbiol, 37, 1591–1594.

    PubMed  CAS  Google Scholar 

  • Chaix, C., Durand-Zaleski, I., Alberti, C., & Brun-Buisson, C. (1999). Control of endemic methicillin-resistant Staphylococcus aureus: a cost-benefit analysis in an intensive care unit. JAMA, 282, 1745–1751.

    Article  PubMed  CAS  Google Scholar 

  • Charbonnier, Y., Gettler, B. M., Francois, P., Bento, M., Renzoni, A., Vaudaux, P., Schlegel, W., & Schrenzel, J. (2005) A generic approach for the design of whole-genome 24. Rapid Screening and Identification of MRSA 421 oligoarrays, validated for genomotyping, deletion mapping and gene expression analysis on Staphylococcus aureus. BMC Genomics, 6.

    Google Scholar 

  • Cohen, S. H., Morita, M. M., & Bradford, M. (1991). A seven-year experience with methicillin-resistant Staphylococcus aureus. Am J Med, 91, 233S–237S.

    Article  PubMed  CAS  Google Scholar 

  • Corless, C. E., Guiver, M., Borrow, R., Edwards-Jones, V., Fox, A. J., & Kaczmarski, E. B. (2001). Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR. J Clin Microbiol, 39, 1553–1558.

    Article  PubMed  CAS  Google Scholar 

  • Cosseron-Zerbib, M., Roque Afonso, A. M., Naas, T., Durand, P., Meyer, L., Costa, Y., Helali, N. el, Huault, G., & Nordmann, P. (1998). A control programme for MRSA (methicillin-resistant Staphylococcus aureus) containment in a paediatric intensive care unit: evaluation and impact on infections caused by other micro-organisms. J Hosp Infect, 40, 225–235.

    Article  PubMed  CAS  Google Scholar 

  • Couzinet, S., Jay, C., Barras, C., Vachon, R., Vernet, G., Ninet, B., Jan, I., Minazio, M. A., Francois, P., Lew, D., Troesch, A., & Schrenzel, J. (2005a). High-density DNA probe arrays for identification of staphylococci to the species level. J Microbiol Methods, 61, 201–208.

    Article  CAS  Google Scholar 

  • Couzinet, S., Yugueros, J., Barras, C., Visomblin, N., Francois, P., Lacroix, B., Vernet, G., Lew, D., Troesch, A., Schrenzel, J., & Jay, C. (2005b). Evaluation of a high-density oligonucleotide array for characterization of grlA, grlB, gyrA and gyrB mutations in fluoroquinolone resistant Staphylococcus aureus isolates. J Microbiol Methods, 60, 275–279.

    Article  CAS  Google Scholar 

  • Cox, R. A., Conquest, C., Mallaghan, C., & Marples, R. R. (1995). A major outbreak of methicillin-resistant Staphylococcus aureus caused by a new phage-type (EMRSA-16). J Hosp Infect, 29, 87–106.

    Article  PubMed  CAS  Google Scholar 

  • Cuny, C., Witte, W. (2005). PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single premier pair specific for SCCmec elements and the neighbouring chromosome-borne orfX. Clin. Microbiol. Infact, 11, 834–837.

    Article  CAS  Google Scholar 

  • Diederen, B., van, D., I, Van Belkum, A., Willemse, P., van Keulen, P., & Kluytmans, J. (2005). Performance of CHROMagar MRSA medium for detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol, 43, 1925–1927.

    Article  PubMed  CAS  Google Scholar 

  • Diekema, D. J., Dodgson, K. J., Sigurdardottir, B., & Pfaller, M. A. (2004). Rapid detection of antimicrobial-resistant organism carriage: an unmet clinical need. J Clin Microbiol, 42, 2879–2883.

    Article  PubMed  Google Scholar 

  • Donnio, P. Y., Oliveira, D. C., Faria, N. A., Wilhelm, N., Le Coustumier, A., & de Lencastre, H. (2005). Partial excision of the chromosomal cassette containing the methicillin resistance determinant results in methicillin-susceptible Staphylococcus aureus. J Clin Microbiol, 43, 4191–4193.

    Article  PubMed  CAS  Google Scholar 

  • Dufour, P., Gillet, Y., Bes, M., Lina, G., Vandenesch, F., Floret, D., Etienne, J., & Richet, H. (2002). Community-acquired methicillin-resistant Staphylococcus aureus infections in France: emergence of a single clone that produces Panton-Valentine leukocidin. Clin Infect Dis, 35, 819–824.

    Article  PubMed  CAS  Google Scholar 

  • Fang, H. & Hedin, G. (2003). Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay. J Clin Microbiol, 41, 2894–2899.

    Article  PubMed  CAS  Google Scholar 

  • Francois, P., Bento, M., Vaudaux, P., & Schrenzel, J. (2003a). Comparison of fluorescence and resonance light scattering for highly sensitive microarray detection of bacterial pathogens. J Microbiol Methods, 55, 755–762.

    Article  CAS  Google Scholar 

  • Francois, P., Pittet, D., Bento, M., Pepey, B., Vaudaux, P., Lew, D., & Schrenzel, J. (2003b). Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay. J Clin Microbiol, 41, 254–260.

    Article  CAS  Google Scholar 

  • Francois, P., Renzi, G., Pittet, D., Bento, M., Lew, D., Harbarth, S., Vaudaux, P., & Schrenzel, J. (2004). A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements. J Clin Microbiol, 42, 3309–3312.

    Article  PubMed  CAS  Google Scholar 

  • Gieseler, S., Konig, B., Konig, W., & Backert, S. (2005). Strain-specific expression profiles of virulence genes in Helicobacter pylori during infection of gastric epithelial cells and granulocytes. Microbes,Infect, 7, 437–447.

    Article  CAS  Google Scholar 

  • Gill, S. R. (2004). Staphylococcus aureus COL genome. Available at http://www.tigr. org/tigr-scripts/CMR2/GenomePage3.spl?database=gsa.

    Google Scholar 

  • Grundmann, H., Barwolff, S., Tami, A., Behnke, M., Schwab, F., Geffers, C., Halle, E., Gobel, U. B., Schiller, R., Jonas, D., Klare, I., Weist, K., Witte, W., Beck-Beilecke, K., Schumacher, M., Ruden, H., & Gastmeier, P. (2005). How many infections are caused by patient-to-patient transmission in intensive care units? Crit Care Med. 33,946–951.

    Article  PubMed  Google Scholar 

  • Hamels, S., Gala, J. L., Dufour, S., Vannuffel, P., Zammatteo, N., & Remacle, J. (2001). Consensus PCR and microarray for diagnosis of the genus Staphylococcus, species, and methicillin resistance. Biotechniques, 31, 1364–2.

    PubMed  CAS  Google Scholar 

  • Harbarth, S. & Pittet, D. (1998). MRSA-a European currency of Infection control. Q J Med, 91, 519–521.

    CAS  Google Scholar 

  • Harbarth, S., Martin, Y., Rohner, P., Henry, N., Auckenthaler, R., & Pittet, D. (2000). Effect of delayed infection control measures on a hospital outbreak of methicillin-resistant Staphylococcus aureus. J Hosp Infect, 46, 43–49.

    Article  PubMed  CAS  Google Scholar 

  • Harbarth, S., Francois, P., Schrenzel, J., Fankhauser-Rodriguez, C., Hugonnet, S., Koessler, T., Huyghe, A., & Pittet, D. (2005). Community-associated methicillin-resistant Staphylococcus aureus, Geneva, Switzerland. Emerg Infect Dis, 11, 962–965.

    PubMed  Google Scholar 

  • He, J. W. & Jiang, S. (2005). Quantification of enterococci and human adenoviruses in environmental samples by real-time PCR. Appl Environ Microbiol, 71, 2250–2255.

    Article  PubMed  CAS  Google Scholar 

  • Herwaldt, L. A. (1999). Control of methicillin-resistant Staphylococcus aureus in the hospital setting. Am J Med, 106, 11S–18S.

    Article  PubMed  CAS  Google Scholar 

  • Hiramatsu, K., Cui, L., Kuroda, M., & Ito, T. (2001). The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends Microbiol, 9, 486–493.

    Article  PubMed  CAS  Google Scholar 

  • Holden, M. T., Feil, E. J., Lindsay, J. A., Peacock, S. J., Day, N. P., Enright, M. C., Foster, T. J., Moore, C. E., Hurst, L., Atkin, R., Barron, A., Bason, N., Bentley, S. D., Chillingworth, C., Chillingworth, T., Churcher, C., Clark, L., Corton, C., Cronin, A., Doggett, J., Dowd, L., Feltwell, T., Hance, Z., Harris, B., Hauser, H., Holroyd, S., Jagels, K., James, K. D., Lennard, N., Line, A., Mayes, R., Moule, S., Mungall, K., Ormond, D., Quail, M. A., Rabbinowitsch, E., Rutherford, K., Sanders, M., Sharp, S., Simmonds, M., Stevens, K., Whitehead, S., Barrell, B. G., Spratt, B. G., & Parkhill, J. (2004). Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance. Proc Natl Acad Sci USA.

    Google Scholar 

  • Hryniewicz, W. (1999). Epidemiology of MRSA. Infection, 27 (Suppl 2), S13–S16.

    Article  PubMed  Google Scholar 

  • Hsu, C. F., Tsai, T. Y., & Pan, T. M. (2005). Use of the duplex TaqMan PCR system for detection of Shiga-like toxin-producing Escherichia coli O157. J Clin Microbiol, 43, 2668–2673.

    Article  PubMed  CAS  Google Scholar 

  • Huletsky, A., Giroux, R., Rossbach, V., Gagnon, M., Vaillancourt, M., Bernier, M., Gagnon, F., Truchon, K., Bastien, M., Picard, F. J., Van Belkum, A., Ouellette, M., Roy, P. H., & Bergeron, M. G. (2004). New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J Clin Microbiol, 42, 1875–1884.

    Article  PubMed  CAS  Google Scholar 

  • Hurlimann-Dalel, R. L., Ryffel, C., Kayser, F. H., & Berger-Bachi, B. (1992). Survey of the methicillin resistance-associated genes mecA, mecR1-mecI, and femA-femB in clinical isolates of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother, 36, 2617–2621.

    PubMed  CAS  Google Scholar 

  • Hussain, Z., Stoakes, L., Garrow, S., Longo, S., Fitzgerald, V., & Lannigan, R. (2000). Rapid detection of mecA-positive and mecA-negative coagulase-negative staphylococci by an anti-penicillin binding protein 2a slide latex agglutination test. J Clin Microbiol, 38, 2051–2054.

    PubMed  CAS  Google Scholar 

  • Ito, T., Katayama, Y., Asada, K., Mori, N., Tsutsumimoto, K., Tiensasitorn, C., & Hiramatsu, K. (2001). Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother, 45, 1323–1336.

    Article  PubMed  CAS  Google Scholar 

  • Ito, T., Okuma, K., Ma, X. X., Yuzawa, H., & Hiramatsu, K. (2003). Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: genomic island SCC. Drug Resist Update, 6, 41–52.

    Article  CAS  Google Scholar 

  • Ito, T., Ma, X. X., Takeuchi, F., Okuma, K., Yuzawa, H., & Hiramatsu, K. (2004). Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrob Agents Chemother, 48, 2637–2651.

    Article  PubMed  CAS  Google Scholar 

  • Katayama, Y., Takeuchi, F., Ito, T., Ma, X. X., Ui-Mizutani, Y., Kobayashi, I. & Hiramatsu, K. (2003). Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus. J Bacteriol, 185, 2711–2722.

    Article  PubMed  CAS  Google Scholar 

  • Kearns, A. M., Seiders, P. R., Wheeler, J., Freeman, R., & Steward, M. (1999). Rapid detection of methicillin-resistant staphylococci by multiplex PCR. J Hosp Infect, 43, 33–37.

    Article  PubMed  CAS  Google Scholar 

  • KKI. (Komimission für Krankenhaushygiene und Infektionspravention) (1999). Empfehlung zur Prävention und Kontrolle von Methicillin-resistenten Staphylococcus aureus- Stämmen (MRSA) in Krankenhäusern und anderen medizinischen Einrichtungen. Bundesgesundheitsbl Gesundheitsforsch Gesundheitsschutz, 42, 954–958.

    Google Scholar 

  • Kluytmans, J., Van Belkum, A., & Verbrugh, H. (1997). Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clin Microbiol Rev, 10, 505–520.

    PubMed  CAS  Google Scholar 

  • Kluytmans, J., van Griethuysen, A., Willemse, P., & van Keulen, P. 2002. Performance of CHROMagar selective medium and oxacillin resistance screening agar base for identifying Staphylococcus aureus and detecting methicillin resistance. J Clin Microbiol, 40, 2480–2482.

    Article  PubMed  CAS  Google Scholar 

  • Kuroda, M., Ohta, T., Uchiyama, I., Baba, T., Yuzawa, H., Kobayashi, I., Cui, L., Oguchi, A., Aoki, K., Nagai, Y., Lian, J., Ito, T., Kanamori, M., Matsumaru, H., Maruyama, A., Murakami, H., Hosoyama, A., Mizutani-Ui, Y., Takahashi, N. K., Sawano, T., Inoue, R., Kaito, C., Sekimizu, K., Hirakawa, H., Kuhara, S., Goto, S., Yabuzaki, J., Kanehisa, M., Yamashita, A., Oshima, K., Furuya, K., Yoshino, C., Shiba, T., Hattori, M., Ogasawara, N., Hayashi, H., & Hiramatsu, K. (2001). Whole genome sequencing of methicillin-resistant Staphylococcus aureus. Lancet, 357, 1225–1240.

    Article  PubMed  CAS  Google Scholar 

  • Lee, L. G., Livak, K. J., Mullah, B., Graham, R. J., Vinayak, R. S., & Woudenberg, T. M. (1999). Seven-color, homogeneous detection of six PCR products. Biotechniques, 27, 342–349.

    PubMed  CAS  Google Scholar 

  • Lee, S. O., Na, D. K., Kim, C. M., Li, Y. H., Cho, Y. H., Park, J. H., Lee, J. H., Eo, S. K., Klein, T. A., & Chae, J. S. (2005). Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene. J Vet Sci, 6, 151–155.

    PubMed  Google Scholar 

  • Louie, L., Goodfellow, J., Mathieu, P., Glatt, A., Louie, M., & Simor, A. E. (2002). Rapid detection of methicillin-resistant staphylococci from blood culture bottles by using a multiplex PCR assay. J Clin Microbiol, 40, 2786–2790.

    Article  PubMed  CAS  Google Scholar 

  • Ma, X. X., Ito, T., Tiensasitorn, C., Jamklang, M., Chongtrakool, P., Boyle-Vavra, S., Daum, R. S., & Hiramatsu, K. (2002). Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob Agents Chemother, 46, 1147–1152.

    Article  PubMed  CAS  Google Scholar 

  • Mackay, I. M., Arden, K. E., & Nitsche, A. (2002). Real-time PCR in virology. Nucleic Acids Res, 30, 1292–1305.

    Article  PubMed  CAS  Google Scholar 

  • Maes, N., Magdalena, J., Rottiers, S., De Gheldre, Y., & Struelens, M. J. (2002). Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative staphylococci and determine methicillin resistance from blood cultures. J Clin Microbiol, 40, 1514–1517.

    Article  PubMed  CAS  Google Scholar 

  • Mason, W. J., Blevins, J. S., Beenken, K., Wibowo, N., Ojha, N., & Smeltzer, M. S. (2001). Multiplex PCR protocol for the diagnosis of staphylococcal infection. J Clin Microbiol, 39, 3332–3338.

    Article  PubMed  CAS  Google Scholar 

  • Masuet-Aumatell, C., Pittet, D., Schrenzel, J., Akapko, C., Renzi, G., Ricou, B., Pugin, J., & Harbarth, S. (2004). Evaluation of a rapid screening strategy for detecting methicillin-resistant Staphylococcus aureus(MRSA) in critical care. Poster D57. 44th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington, DC.

    Google Scholar 

  • Murakami, K., Minamide, W., Wada, K., Nakamura, E., Teraoka, H., & Watanabe, S. (1991). Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J Clin Microbiol, 29, 2240–2244.

    PubMed  CAS  Google Scholar 

  • Muto, C. A., Jernigan, J. A., Ostrowsky, B. E., Richet, H. M., Jarvis, W. R., Boyce, J. M., & Farr, B. M. (2003). SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and enterococcus. Infect Control Hosp Epidemiol, 24, 362–386.

    Article  PubMed  Google Scholar 

  • Naimi, T. S., LeDell, K. H., Como-Sabetti, K., Borchardt, S. M., Boxrud, D. J., Etienne, J., Johnson, S. K., Vandenesch, F., Fridkin, S., O’Boyle, C., Danila, R. N., & Lynfield, R. (2003). Comparison of community- and health care-associated methicillin-resistant Staphylococcus aureus infection. JAMA 290, 2976–2984.

    Article  PubMed  CAS  Google Scholar 

  • NCCLS (National Committee for Clinical Laboratory Standards). (2002). Performance standards for antimicrobial susceptibility testing. NCCLS approved standard M100–S11. National Committee for Clinical Laboratory Standards, Wayne, PA.

    Google Scholar 

  • Nettleman, M. D., Trilla, A., Fredrickson, M., & Pfaller, M. (1991). Assigning responsibility: using feedback to achieve sustained control of methicillin-resistant Staphylococcus aureus. Am J Med, 91, 228S–232S.

    Article  PubMed  CAS  Google Scholar 

  • Nolte, F. S. & Calidendo, A. M. (2003). Molecular detection and identification of microorganisms. In: Murray, P. R., Barron, E. J., Jorgensen,J. H., Pfaller, M. A., & Yolken R. H., eds. Manual of Clinical Microbiology. ASM Press, Washington DC, pp. 234–256.

    Google Scholar 

  • Papia, G., Louie, M., Tralla, A., Johnson, C., Collins, V., & Simor, A. E. (1999). Screening high-risk patients for methicillin-resistant Staphylococcus aureus on admission to the hospital, is it cost effective? Infect Control Hosp Epidemiol, 20, 473–477.

    Article  CAS  Google Scholar 

  • Perry, J. D., Davies, A., Butterworth, L. A., Hopley, A. L., Nicholson, A., & Gould, F. K. (2004). Development and evaluation of a chromogenic agar medium for methicillin-resistant Staphylococcus aureus. J Clin Microbiol, 42, 4519–4523.

    Article  PubMed  CAS  Google Scholar 

  • Pittet, D. & Waldvogel, F. A. (1997). To control or not control colonization with MRSA … that’s the question! Q J Med, 90, 239–241.

    CAS  Google Scholar 

  • Pittet, D., Safran, E., Harbarth, S., Borst, F., Copin, P., Rohner, P., Scherrer, J. R., & Auckenthaler, R. (1996). Automatic alerts for methicillin-resistant Staphylococcus aureus surveillance and control: role of a hospital information system. Infect Control Hosp Epidemiol, 17, 496–502.

    Article  PubMed  CAS  Google Scholar 

  • Pittet, D., Hugonnet, S., Harbarth, S., Mourouga, P., Sauvan, V., Touveneau, S., & Perneger, T. V. (2000). Effectiveness of a hospital-wide programme to improve compliance with hand hygiene. Infection Control Programme. Lancet, 356, 1307–1312.

    Article  PubMed  CAS  Google Scholar 

  • Richardson, J. F. & Reith, S. (1993). Characterization of a strain of methicillin-resistant Staphylococcus aureus (EMRSA-15) by conventional and molecular methods. J Hosp Infect, 25, 45–52.

    Article  PubMed  CAS  Google Scholar 

  • Rohrer, S., Tschierske, M., Zbinden, R., & Berger-Bachi, B. (2001). Improved methods for detection of methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis, 20, 267–270.

    Article  PubMed  CAS  Google Scholar 

  • Rubinovitch, B. & Pittet, D. (2001). Screening for methicillin-resistant Staphylococcus aureus in the endemic hospital: what have we learned? J Hosp Infect, 47, 9–18.

    Article  CAS  Google Scholar 

  • Ryffel, C., Tesch, W., Birch-Machin, I., Reynolds, P. E., Barberis-Maino, L., Kayser, F. H., & Berger-Bachi, B. (1990). Sequence comparison of mecA genes isolated from methicillinresistant Staphylococcus aureus and Staphylococcus epidermidis. Gene 94, 137–138.

    Article  PubMed  CAS  Google Scholar 

  • Ryffel, C., Kayser, F. H., & Berger-Bachi, B. (1992). Correlation between regulation of mecA transcription and expression of methicillin resistance in staphylococci. Antimicrob Agents Chemother, 36, 25–31.

    PubMed  CAS  Google Scholar 

  • Said-Salim, B., Mathema, B., & Kreiswirth, B. N. (2003). Community-acquired methicillinresistant Staphylococcus aureus: an emerging pathogen. Infect Control Hosp Epidemiol, 24, 451–455.

    Article  PubMed  Google Scholar 

  • Saiman, L., O’Keefe, M., Graham, P. L., III, Wu, F., Said-Salim, B., Kreiswirth, B., LaSala, A., Schlievert, P. M., & Della-Latta, P. (2003). Hospital transmission of community-acquired methicillin-resistant Staphylococcus aureus among postpartum women. Clin Infect Dis, 37, 1313–1319.

    Article  PubMed  Google Scholar 

  • Sakoulas, G., Eliopoulos, G. M., Moellering, R. C., Jr., Wennersten, C., Venkataraman, L., Novick, R. P., & Gold, H. S. (2002). Accessory gene regulator (agr) locus in geographically diverse Staphylococcus aureus isolates with reduced susceptibility to vancomycin. Antimicrob Agents Chemother, 46, 1492–1502.

    Article  PubMed  CAS  Google Scholar 

  • Saunders, N. A., Underwood, A., Kearns, A. M., & Hallas, G. (2004). A virulenceassociated gene microarray: a tool for investigation of the evolution and pathogenic potential of Staphylococcus aureus. Microbiology 150, 3763–3771.

    Article  PubMed  CAS  Google Scholar 

  • Schmitz, F. J., Steiert, M., Hofmann, B., Verhoef, J., Hadding, U., Heinz, H. P., & Kohrer, K. (1998). Development of a multiplex-PCR for direct detection of the genes for enterotoxin B and C, and toxic shock syndrome toxin- in Staphylococcus aureus isolates. J Med Microbiol, 47, 335–340.

    PubMed  CAS  Google Scholar 

  • Simor, A. E., Goodfellow, J., Louie, L., & Louie, M. (2001). Evaluation of new medium, oxacillin resistance screening agar base, for the detection of methicillin-resistant Staphylococcus aureus from clinical specimens. J Clin Microbiol, 39, 3422.

    Article  PubMed  CAS  Google Scholar 

  • Svenstrup, H. F., Jensen, J. S., Bjornelius, E., Lidbrink, P., Birkelund, S., & Christiansen, G. (2005). Development of a quantitative real-time PCR assay for detection of Mycoplasma genitalium. J Clin Microbiol, 43, 3121–3128.

    Article  PubMed  CAS  Google Scholar 

  • Towner, K. J., Talbot, D. C., Curran, R., Webster, C. A., & Humphreys, H. (1998). Development and evaluation of a PCR-based immunoassay for the rapid detection of methicillin-resistant Staphylococcus aureus. J Med Microbiol, 47, 607–613.

    Article  PubMed  CAS  Google Scholar 

  • Vandenesch, F., Naimi, T., Enright, M. C., Lina, G., Nimmo, G. R., Heffernan, H., Liassine, N., Bes, M., Greenland, T., Reverdy, M. E., & Etienne, J. (2003). Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence. Emerg Infect Dis, 9, 978–984.

    PubMed  Google Scholar 

  • Vannuffel, P., Laterre, P. F., Bouyer, M., Gigi, J., Vandercam, B., Reynaert, M., & Gala, J. L. (1998). Rapid and specific molecular identification of methicillin-resistant Staphylococcus aureus in endotracheal aspirates from mechanically ventilated patients. J Clin Microbiol, 36, 2366–2368.

    PubMed  CAS  Google Scholar 

  • Wernitz, M. H., Swidsinski, S., Weist, K., Sohr, D., Witte, W., Franke, K. P., Roloff, D., Ruden, H., & Veit, S. K. (2005). Effectiveness of a hospital-wide selective screening programme for methicillin-resistant Staphylococcus aureus (MRSA) carriers at hospital admission to prevent hospital-acquired MRSA infections. Clin Microbiol Infect, 11, 457–465.

    Article  PubMed  CAS  Google Scholar 

  • Wielders, C. L., Vriens, M. R., Brisse, S., Graaf-Miltenburg, L. A., Troelstra, A., Fleer, A., Schmitz, F. J., Verhoef, J., & Fluit, A. C. (2001). Evidence for in-vivo transfer of mecA DNA between strains of Staphylococcus aureus. Lancet, 357,1674–1675.

    Article  PubMed  CAS  Google Scholar 

  • Youmans, G. R., Davis, T. E., & Fuller, D. D. (1993). Use of chemiluminescent DNA probes in the rapid detection of oxacillin resistance in clinically isolated strains of Staphylococcus aureus. Diagn Microbiol Infect Dis, 16, 99–104.

    Article  PubMed  CAS  Google Scholar 

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Francois, P., Schrenzel, J. (2006). Rapid Screening and Identification of Methicillin-Resistant Staphylococcus aureus. In: Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/0-387-32892-0_24

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