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Direct Nucleotide Sequencing for Amplification Product Identification

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Advanced Techniques in Diagnostic Microbiology

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Abstract

The advances of technology to determine the nucleotide sequence of DNA have fundamentally changed the field of biological research and medicine. For diagnostic molecular microbiology, the most precise method of identification of a PCR product (amplicon) is to determine its nucleotide sequence. Although it is not always necessary to sequence the entire amplicon for routine diagnostic procedures, DNA sequence has been used to analyze a broad range of PCR products for bacterial identification; for gene mutations related to antimicrobial resistance; for bacterial strain typing and viral genotyping; and so forth. Most of the amplicons of these applications are large (range approximately from 300 base pairs to 1500 base pairs), and the exact nucleotide sequence of the amplicoms are crucial for the results.

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  1. Tao Hong
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© 2006 Springer

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Hong, T. (2006). Direct Nucleotide Sequencing for Amplification Product Identification. In: Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/0-387-32892-0_16

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