5. Conclusions
FAP and DPIV overexpression in the HEK293 cell line reduced cell adhesion, migration and invasion on ECM components, increased proliferation and promoted apoptosis, independently of enzyme activity. These observations may aid an understanding of the roles of FAP and DPIV in tissue remodeling.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abbott CA, McCaughan GW, Gorrell MD, 1999, Two highly conserved glutamic acid residues in the predicted beta propeller domain of dipeptidyl peptidase IV are required for its enzyme activity. FEBS Lett. 458: 278–284.
Aertgeerts K, Levin I, Shi L, Snell GP, Jennings A, Prasad GS, Zhang Y, Kraus ML, Salakian S, Sridhar V, Wijnands R, Tennant MG, 2005, Structural and kinetic analysis of the substrate specificity of human fibroblast activation protein. J Biol. 280: 19441–41944.
Ajami K, Abbott CA, Obradovic M, Gysbers V, Kähne T, McCaughan GW, Gorrell MD, 2003, Structural requirements for catalysis, expression and dimerisation in the CD26/DPIV gene family. Biochemistry. 42: 694–701.
Ajami K, Abbott CA, McCaughan GW, Gorrell MD, 2004, Dipeptidyl peptidase 9 is cytoplasmic and has two forms, a broad tissue distribution, cytoplasmic localization and DPIV—like peptidase activity. BBA—Gene Struct Expression. 1679: 18–28.
Alahari SK, Lee JW, Juliano RL, 2000, Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration. J Cell Biol. 151: 1141–1154.
Aldinucci D, Poletto D, Lorenzon D, Nanni P, Degan M, Olivo K, Rapana B, Pinto A, Gattei V, 2004, CD26 expression correlates with a reduced sensitivity to 2′-deoxycoformycin-induced growth inhibition and apoptosis in T-cell leukemia/lymphomas. Clin Cancer Res. 10: 508–520.
Cheng HC, Abdel-Ghany M, Pauli BU, 2003, A novel consensus motif in fibronectin mediates dipeptidyl peptidase IV adhesion and metastasis. J Biol Chem. 278: 24600–24607.
Hanski C, Huhle T, Reutter W, 1985, Involvement of plasma membrane dipeptidyl peptidase IV in fibronectin-mediated adhesion of cells on collagen. Biol Chem Hoppe Seyler. 366: 1169–1176.
Kajiyama H, Kikkawa F, Khin E, Shibata K, Ino K, Mizutani S, 2003, Dipeptidyl peptidase IV overexpression induces up-regulation of E-cadherin and tissue inhibitors of matrix metalloproteinases, resulting in decreased invasive potential in ovarian carcinoma cells. Cancer Res. 63: 2278–2283.
Levy MT, McCaughan GW, Abbott CA, Park JE, Cunningham AM, Rettig WJ, Gorrell MD, 1999, Fibroblast activation protein: A cell surface dipeptidyl peptidase and gelatinase expressed by stellate cells at the tissue remodelling interface in human cirrhosis. Hepatology. 29: 1768–1778.
Levy MT, McCaughan GW, Marinos G, Gorrell MD, 2002, Intrahepatic expression of the hepatic stellate cell marker fibroblast activation protein correlates with the degree of fibrosis in hepatitis C virus infection. Liver. 22: 93–101.
Luo J, Lubaroff DM, Hendrix MJ, 1999, Suppression of prostate cancer invasive potential and matrix metalloproteinase activity by E-cadherin transfection. Cancer Res. 59: 3552–3556.
Matsumoto Y, Bishop GA, McCaughan GW, 1992, Altered zonal expression of the CD26 antigen in human cirrhotic liver. Hepatology. 15: 1048–1053.
Matsuyama W, Wang L, Farrar WL, Faure M, Yoshimura T, 2004, Activation of discoidin domain receptor 1 isoform b with collagen up-regulates chemokine production in human macrophages: role of p38 mitogen-activated protein kinase and NF-kappa B. J Immunol. 172: 2332–2340.
Mizokami Y, Kajiyama H, Shibata K, Ino K, Kikkawa F, Mizutani S, 2004, Stromal cell-derived factor-1alphainduced cell proliferation and its possible regulation by dipeptidyl peptidase IV in endometrial adenocarcinoma. Int J Cancer. 110: 652–659.
Mueller SC, Ghersi G, Akiyama SK, Sang QXA, Howard L, Pineiro-Sanchez M, Nakahara H, Yeh Y, Chen WT, 1999, A novel protease-docking function of integrin at invadopodia. J Biol Chem. 274: 24947–24952.
Park JE, Lenter MC, Zimmermann RN, Garin-Chesa P, Old LJ, Rettig WJ, 1999, Fibroblast activation protein: A dual-specificity serine protease expressed in reactive human tumor stromal fibroblasts. J Biol Chem. 274: 36505–36512.
Pethiyagoda CL, Welch DR, Fleming TP, 2001, Dipeptidyl peptidase IV (DPPIV) inhibits cellular invasion of melanoma cells. Clin Exp Metastasis. 18: 391–400.
Ramirez-Montagut T, Blachere NE, Sviderskaya EV, Bennett DC, Rettig WJ, Garin-Chesa P, Houghton AN, 2004, FAPalpha, a surface peptidase expressed during wound healing, is a tumor suppressor. Oncogene. 23: 5435–5446.
Sato K, Aytac U, Yamochi T, Ohnuma K, McKee KS, Morimoto C, Dang NH, 2003, CD26 enhances expression of topoisomerase II alpha and sensitivity to apoptosis induced by topoisomerase II inhibitors. Br J Cancer. 89: 1366–1374.
Shackel NA, McGuinness PH, Abbott CA, Gorrell MD, McCaughan GW, 2002, Insights into the pathobiology of hepatitis C virus associated cirrhosis: Analysis of intrahepatic differential gene expression. Am J Pathol. 160: 641–654.
Slimane TA, Lenoir C, Bello V, Delaunay JL, Goding JW, Chwetzoff S, Maurice M, Fransen JAM, Trugnan G, 2001, The cytoplasmic/transmembrane domain of dipeptidyl peptidase IV, a type II glycoprotein, contains an apical targeting signal that does not specifically interact with lipid rafts. Exp Cell Res. 270: 45–55.
Wesley UV, Albino A, Tiwari S, Houghton A, 1999, A role for dipeptidyl peptidase IV in suppressing the malignant phenotype of melanocytic cells. J Exp Med. 190: 311–322.
Wesley UV, Tiwari S, Houghton AN, 2004, Role for dipeptidyl peptidase IV in tumor suppression of human non small cell lung carcinoma cells. Int J Cancer. 109: 855–866.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Springer Science+Business Media, Inc.
About this paper
Cite this paper
Wang, X.M., Yu, D.M.T., McCaughan, G.W., Gorrell, M.D. (2006). Extra-Enzymatic Roles of DPIV and FAP in Cell Adhesion and Migration on Collagen and Fibronectin. In: Lendeckel, U., Reinhold, D., Bank, U. (eds) Dipeptidyl Aminopeptidases. Advances in Experimental Medicine and Biology, vol 575. Springer, Boston, MA . https://doi.org/10.1007/0-387-32824-6_23
Download citation
DOI: https://doi.org/10.1007/0-387-32824-6_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-29058-4
Online ISBN: 978-0-387-32824-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)