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Prolyl Endopeptidase Cleaves the Apoptosis Rescue Peptide Humanin and Exhibits an Unknown Post-Cysteine Cleavage Specificity

  • Joachim Wolfgang Bär
  • Jens-Ulrich Rahfeld
  • Ingo Schulz
  • Kathrin Gans
  • David Ruiz-Carrillo
  • Susanne Manhart
  • Fred Rosche
  • Hans-Ulrich Demuth
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (volume 575)

4. Conclusion

The data presented, demonstrate for the first time PEP turnover of HN by a limited postcysteine as well as the expected post-proline proteolysis demonstrated in cell extract resulting in the inactivation of this potentially apoptosis-related factor. These findings lead to the hypothesis of a PEP-mediated control of HN homeostasis maintaining neuronal cell survival. This implicates a novel use of PEP inhibitors potentially preventing intracellular HN digestion. Consequently, PEP-inhibition might be a new target for apoptosis prevention.

This study further uncovered a so far unknown enzymatic specificity for a post-cysteine cleavage of the mammalian exopeptidases DP2, DP4, DP8 and DP9 and the endopeptidase PEP.

Keywords

Dipeptidyl Peptidase Prolyl Endopeptidase Prolyl Oligopeptidase Prolyl Endopeptidase Inhibition Apoptosis Prevention 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Joachim Wolfgang Bär
    • 1
  • Jens-Ulrich Rahfeld
    • 1
  • Ingo Schulz
    • 1
  • Kathrin Gans
    • 1
  • David Ruiz-Carrillo
    • 1
  • Susanne Manhart
    • 1
  • Fred Rosche
    • 1
  • Hans-Ulrich Demuth
    • 1
  1. 1.Departments of Molecular Biology & EnzymologyProbiodrug AGHalle (Saale)Germany

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