Dipeptidyl Peptidase 8 Has Post-Proline Dipeptidyl Aminopeptidase and Prolyl Endopeptidase Activities
We report an expression and purification procedure to produce homogeneous, active human DP8 and DPIV. The most interesting discovery was that DP8 specifically has both prolyl dipeptidyl aminopeptidase and PEP activities. DPIV inhibitors varied in their selectivity against DP8 but all DP8 activities were inhibited by the irreversible DPIV inhibitor ValboroPro. These data indicate that DP8 is a multifunctional enzyme and that therapeutics based on DPIV inhibition should be counter-screened against DP8.
KeywordsCeliac Disease Cell Culture Supernatant Dipeptidyl Peptidase Prolyl Endopeptidase Prolyl Oligopeptidase
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