Advertisement

Voltage-Dependent K Channels in Mouse Glomus Cells are Modulated by Acetylcholine

  • TOSHIKI OTSUBO
  • SHIGEKI YAMAGUCHI
  • MACHIKO SHIRAHATA
Part of the ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY book series (AEMB, volume 580)

Abstract

Several neurotransmitters are present in the carotid body. These neurotransmitters are responsible to evoke action potentials in afferent nerve endings. They also modify the function of glomus cells, by binding to autoreceptors on glomus cells. We have previously demonstrated that ACh variably influences voltage-dependent K (Kv) current in cat glomus cells (Shirahata et al., 2002). Excitability of glomus cells are regulated by several types of K channels including voltage-dependent (Kv) channels (Buckler, 1999; Shirahata and Sham, 1999). Kv channels are activated with membrane depolarization, and play an essential role for repolarizing the cell membrane. Several studies have shown that the activity of Kv channels are modulated by neurotransmitters (Brown et al., 1997; Dong and White, 2003; Fukuda et al., 1988; Huang et al., 1993; Shi et al., 1999, 2004), and this type of modification may be important for fine tuning of the excitability of glomus cells. Recent studies have shown that the carotid body of DBA/2J inbred strain of mice demonstrates morphological similarities to cat glomus cells (Yamaguchi et al., 2003). Glomus cells of these mice responds to ACh (Yamaguchi et al., 2003) and express 4-aminopyridine sensitive and charybdotoxin sensitive components of Kv channels (Yamaguchi et al., 2004). In this study, we have investigated whether Kv channels in glomus cells of DBA/2J mice are also modified by ACh. Further, some underlying mechanisms of Kv current modulation by ACh was also investigated.

Keywords

Carotid Body Krebs Solution Tyrode Solution Glomus Cell Cholinergic Modulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brown D.A., Abogadie F.C., Allen T.G., Buckley N.J., Caulfield M.P., Delmas P., Haley, J.E., Lamas, J.A., Selyanko, A.A., 1997. Muscarinic mechanisms in nerve cells. Life Sci. 60: 1137–1144.PubMedCrossRefGoogle Scholar
  2. Buckler K.J., 1999. Background leak K+-currents and oxygen sensing in carotid body type 1 cells. Respir. Physiol. 115: 179–187.PubMedCrossRefGoogle Scholar
  3. Dong Y., White F.J., 2003. Dopamine D1-class receptors selectively modulate a slowly inactivating potassium current in rat medial prefrontal cortex pyramidal neurons. J. Neurosci. 23: 2686–2695.PubMedGoogle Scholar
  4. Fitzgerald R.S., 2000. Oxygen and carotid body chemotransduction: the cholinergic hypothesis - a brief history and new evaluation. Respir. Physiol. 120: 89–104.PubMedCrossRefGoogle Scholar
  5. Fitzgerald R.S., Shirahata M., Ide T., 1997. Further cholinergic aspects of carotid body chemotransduction of hypoxia in cats. J. Appl. Physiol. 82: 819–827.PubMedGoogle Scholar
  6. Fitzgerald R.S., Shirahata M., 1994. Acetylcholine and carotid body excitation during hypoxia in the cat. J. Appl. Physiol. 76: 1566–1574.PubMedGoogle Scholar
  7. Fitzgerald R.S., Shirahata M., Wang H.Y., 1999. Acetylcholine release from cat carotid bodies. Brain Res. 841: 53–61.PubMedCrossRefGoogle Scholar
  8. Fukuda K., Higashida H., Kubo T., Maeda A., Akiba I., Bujo H., Mishima M., Numa S., 1988. Selective coupling with K+ currents of muscarinic acetylcholine receptor subtypes in NG108–15 cells. Nature 335: 355–358.PubMedCrossRefGoogle Scholar
  9. Higashi T., McIntosh J.M., Shirahata M., 2003. Characterization of nicotinic acetylcholine receptors in cultured arterial chemoreceptor cells of the cat. Brain Res., 974: 167–175.PubMedCrossRefGoogle Scholar
  10. Huang X.Y., Morielli A.D., Peralta E.G., 1993. Tyrosine kinase-dependent suppression of a potassium channel by the G protein-coupled m1 muscarinic acetylcholine receptor. Cell, 75: 1145–1156.PubMedCrossRefGoogle Scholar
  11. Kim D.K., Prabhakar N.R., Kumar G.K., 2004. Acetylcholine release from the carotid body by hypoxia: evidence for the involvement of autoinhibitory receptors. J. Appl. Physiol., 96: 376–383.PubMedCrossRefGoogle Scholar
  12. McGehee D.S., Role L.W., 1995. Physiological diversity of nicotinic acetylcholine receptors expressed by vertebrate neurons. Annu. Rev. Physiol., 57: 521–546.PubMedCrossRefGoogle Scholar
  13. Shi H., Wang H., Wang Z., 1999. M3 muscarinic receptor activation of a delayed rectifier potassium current in canine atrial myocytes. Life Sci., 64: L251–L257.Google Scholar
  14. Shi H., Wang H., Yang B., Xu D., Wang Z., 2004. The M3 receptor-mediated K+ current (IKM3), a Gq protein-coupled K+ channel. J. Biol. Chem., 279: 21774–21778.PubMedCrossRefGoogle Scholar
  15. Shirahata M., Higashi T., Hirasawa S., Yamaguchi S., Fitzgerald R.S., Lande B., 2002. Excitation of Glomus Cells: Interaction between Voltage-gated K+ Channels and Cholinergic Receptors. In: Oxygen Sensing: Responses and Adaptaion to Hypoxia. (Lahiri S., Semenza G.L., Prabhakar N.R., eds), pp 365–379. New York: Marcel Dekker.Google Scholar
  16. Shirahata M., Sham J.S., 1999. Roles of ion channels in carotid body chemotransmission of acute hypoxia. Jpn. J. Physiol. 49: 213–228.PubMedCrossRefGoogle Scholar
  17. Williams S.E., Wootton P., Mason H.S., Bould J., Iles D.E., Riccardi D., Peers C., Kemp P.J., 2004. Hemoxygenase-2 is an oxygen sensor for a calcium-sensitive potassium channel. Science, 306: 2093–2097.PubMedCrossRefGoogle Scholar
  18. Yamaguchi S., Balbir A., Schofield B., Coram J., Tankersley C.G., Fitzgerald R.S., O'Donnell C.P., Shirahata M., 2003. Structural and functional differences of the carotid body between DBA/2J and A/J strains of mice. J. Appl. Physiol. 94: 1536–1542.PubMedGoogle Scholar
  19. Yamaguchi S., Lande B., Kitajima T., Hori Y., Shirahata M., 2004. Patch clamp study of mouse glomus cells using a whole carotid body. Neurosc. Lett. 357: 155–157.CrossRefGoogle Scholar
  20. Zhang M., Fearon I.M., Zhong H., Nurse C.A. 2003. Presynaptic modulation of rat arterial chemoreceptor function by 5-HT: role of K+ channel inhibition via protein kinase C. J. Physiol. 551: 825–842.PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • TOSHIKI OTSUBO
    • 1
  • SHIGEKI YAMAGUCHI
    • 1
  • MACHIKO SHIRAHATA
    • 1
  1. 1.Departments of Environmental Health SciencesJohns Hopkins Bloomberg School of Public HealthBaltimoreUSA

Personalised recommendations