Potential Role of Mitochondria in Hypoxia Sensing by Adrenomedullary Chromaffin Cells
Part of the
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
book series (AEMB, volume 580)
Exposure of the neonate to episodes of acute hypoxia during birth results in a variety of adaptive changes that include fluid re-absorption and secretion of surfactant in the lungs to promote air breathing (Slotkin and Seidler 1988). These physiological responses depend critically on catecholamine secretion from adrenomedullary chromaffin cells (AMC), which express a direct, developmentally-regulated hypoxia sensing mechanism, independent of the nervous system (Slotkin and Seidler 1988, 1986; Thompson et al., 1997). The hypoxic response in neonatal AMC, as well as their immortalized counterparts (i.e. MAH cells), appears to be mediated via inhibition of O2-sensitive K+ channels, though the signaling pathway is not completely understood (Fearon et al 2002; Thompson et al., 1997). These O2 -sensitive K+ channels include large conductance Ca2+-dependent K+, i.e. BK or maxi-K+, and delayed rectifier K+ channels (Thompson and Nurse 1998; Thompson et al., 2002). Inhibition of these channels is thought to facilitate membrane depolarization, voltage-gated Ca2+ entry and catecholamine secretion (Thompson et al., 1997; Thompson and Nurse, 1998, 2000).
KeywordsElectron Transport Chain Reactive Oxygen Species Level Chromaffin Cell Hypoxic Pulmonary Vasoconstriction Catecholamine Secretion
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Buttigieg J, Nurse C (2004). Detection of hypoxia-evoked ATP release from chemoreceptor cells of the rat carotid body. Biochem. Biophys. Res. Commun.
: 82–7.PubMedCrossRefGoogle Scholar
Fearon IM, Thompson RJ, Samjoo I, Vollmer C, Doering LC, Nurse CA. (2002). O2
channels in immortalized rat chromaffin-cell-derived MAH cells. J Physiol.
: 807–18.PubMedCrossRefGoogle Scholar
Fu XW, Wang D, Nurse CA, Dinauer MC, Cutz E. (2000). NADPH oxidase is an O2
sensor in airway chemoreceptors: Evidence from K+
current modulation in wild-type and oxidase-deficient mice. Proc. Nat. Acad. Sci. USA
: 4374–4379.PubMedCrossRefGoogle Scholar
Lopez-Barneo J, Pardal R, Ortega-Saenz P. (2001). Cellular mechanism of oxygen sensing. Annu Rev Physiol.
: 259–87.PubMedCrossRefGoogle Scholar
Michelakis ED, Thebaud B, Weir EK, Archer SL (2004). Hypoxic pulmonary vasoconstriction: redox regulation of O2
channels by a mitochondrial O2
-sensor in resistance artery smooth muscle cells. J Mol Cell Cardiol.
: 1119–36.PubMedGoogle Scholar
Michelakis ED, Hampl V, Nsair A, Wu XC, Harry G, Haromy A, Gurtu R, Archer SL. (2002). Diversity in mitochondria function explains differences in vascular oxygen sensing. Circ. Res.
: 1307–1215.PubMedCrossRefGoogle Scholar
Ortega-Saenz P, Pardal R, Garcia-Fernandez M, Lopez-Barneo J. (2003). Rotenone selectively occludes sensitivity to hypoxia in rat carotid body glomus cells. J Physiol.
: 789–800.PubMedCrossRefGoogle Scholar
Park KS, Nam KJ, Kim JW, Lee YB, Han CY, Jeong JK, Lee HK, Pak YK (2001). Depletion of mitochondrial DNA alters glucose metabolism in SK-Hep1 cells. Am J Physiol Endocrinol Metab.
: E1007–14.PubMedGoogle Scholar
Seidler FJ, Slotkin TA. (1986). Ontogeny of adrenomedullary responses to hypoxia and hypoglycemia: role of splanchnic innervation. Brain Res Bull.
(1): 11–4.CrossRefGoogle Scholar
Slotkin TA, Seidler FJ (1988). Adrenomedullary catecholamine release in the fetus and newborn: secretory mechanisms and their role in stress and survival. J. Dev. Physiol.
: 1–16.PubMedGoogle Scholar
Starkov AA, Fiskum G, Chinopoulos C, Lorenzo BJ, Browne SE, Patel MS, Beal MF. (2004). Mitochondrial alpha-ketoglutarate dehydrogenase complex generates reactive oxygen species. J Neurosci.
(36): 7779–88.PubMedCrossRefGoogle Scholar
Thompson, R.J., Jackson, A. and Nurse, C.A. (1997) Developmental loss of hypoxic chemosensitivity in rat adrenomedullary chromaffin cells. J. Physiol. (Lond)
: 503–510.Google Scholar
Thompson RJ, Nurse CA (1998). Anoxia differentially modulates multiple K+
currents in neonatal rat adrenal chromaffin cells. J. Physiol. (Lond)
: 421–434.CrossRefGoogle Scholar
Thompson, RJ, Nurse CA (2000). O2
-chemosensitivity in developing rat adrenal chromaffin cells. Adv Exp Med Biol.
: 601–9.PubMedCrossRefGoogle Scholar
Thompson, R.J., Farragher, S.M., Cutz, E. and Nurse, C.A. (2002) Developmental regulation of O2
sensing in neonatal adrenal chromaffin cells from wild-type and NADPH-oxidase-deficient mice Pflugers. Arch.
: 539–48.PubMedCrossRefGoogle Scholar
Ward JP, Snetkov VA, Aaronson PI (2004). Calcium, mitochondria and oxygen sensing in the pulmonary circulation. Cell Calcium.
: 209–20.PubMedCrossRefGoogle Scholar
Waypa GB, Chandel NS, Schumacker PT (2001). Model for hypoxic pulmonary vasoconstriction involving mitochondrial oxygen sensing. Circ Res.
: 1259–1266.PubMedGoogle Scholar
Winkler H, Westhead E. (1980). The molecular organization of adrenal chromaffin granules. Neuroscience
: 1803–23.PubMedCrossRefGoogle Scholar