4. Conclusions
A fiber-optic biosensing system for the simultaneous quantification of four cardiac markers is currently under development, in our research group, for rapid coronary disease diagnosis and prognosis. As an initial effort, four individual BNP, cTnI, MG, and CRP sensors were developed. The sensors showed excellent performance in quantifying these cardiac markers in their clinically significant ranges within 15 minutes, at a S/N ratio of 25–50. The cross-reactivity of the four sensors was also found to be negligible. A mixture of four AF647-2o Mabs has shown only a slight interference to the four sensors, indicating that the mixture can be applied through a multi-sensing unit for simultaneous detection with an easier operation.
Other future work includes simultaneous quantification of these four cardiac markers using a multi-sensing unit. A microfluidic system will be incorporated in the unit for precise fluid control. Micro-electro-mechanical systems (MEMS) technology will be utilized for the development of an automatic, smaller, and more cost-effective biosensing chip.
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Tang, L., Kang, K.A. (2006). Preliminary Study of Fiber Optic Multi-Cardiac-Marker Biosensing System for Rapid Coronary Heart Disease Diagnosis and Prognosis. In: Cicco, G., Bruley, D.F., Ferrari, M., Harrison, D.K. (eds) Oxygen Transport to Tissue XXVII. Advances in Experimental Medicine and Biology, vol 578. Springer, Boston, MA . https://doi.org/10.1007/0-387-29540-2_16
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