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Repression of Transcription by Curved DNA and Nucleoid Protein H-NS

A Mode of Bacterial Gene Regulation

  • Chapter
DNA Conformation and Transcription

Part of the book series: Molecular Biology Intelligence Unit ((MBIU))

Abstract

Nucleoid-associated protein H-NS has emerged as one of the most intriguing and versatile global regulators of enterobacterial gene expression acting primarily yet not exclusively at the transcriptional level where it generally acts as a repressor. H-NS is also believed to contribute to the architectural organization of the nucleoid by causing DNA compaction, although the evidence for such a role is not overwhelming. H-NS binds preferentially to DNA elements displaying intrinsic curvatures and can induce DNA bending. These functions are determined by its quaternary tetrameric structure. In turn, the existence of an intrinsic DNA curvature separating two or more H-NS binding sites seems to be characteristic of the H-NS-sensitive promoters and a prerequisite for the transcriptional repressor activity of this protein. In some cases, like that of the virF promoter, the temperature-sensitivity of the DNA curvature represents a key element in the thermo-regulation of pathogenicity gene expression.

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  1. Department of Biology MCA, University of Camerino Camerino (MC), Italy

    Cynthia L. Pon, Stefano Stella & Claudio O. Gualerzi

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Pon, C.L., Stella, S., Gualerzi, C.O. (2005). Repression of Transcription by Curved DNA and Nucleoid Protein H-NS. In: DNA Conformation and Transcription. Molecular Biology Intelligence Unit. Springer, Boston, MA. https://doi.org/10.1007/0-387-29148-2_4

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