Abstract
This chapter addresses the basic applications of tract-tracing and preembedding immunoperoxidase and immunogold-silver labeling for transmission electron microscopy, focusing primarily on identifying the cellular and subcellular localization of proteins of relevance to neurotransmission and on defining synaptic connectivity within neuronal circuits. Information is provided regarding the use of preembedding immunoperoxidase and immunogold techniques to identify the cellular and subcellular localization of neuronal receptors and transporters. The chapter also describes in detail a triple-labeling approach designed by our laboratory for identifying synaptic inputs to neuronal cell populations defined both by their projection targets and by their transmitter phenotype. Protocols presented in the Appendix are designed to enable researchers trained in small animal surgery, immunocytochemistry, electron microscopy, and appropriate laboratory safety procedures to perform ultrastructural investigations similar to those described here.
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Sesack, S.R., Miner, L.H., Omelchenko, N. (2006). Preembedding Immunoelectron Microscopy: Applications for Studies of the Nervous System. In: Zaborszky, L., Wouterlood, F.G., Lanciego, J.L. (eds) Neuroanatomical Tract-Tracing 3. Springer, Boston, MA . https://doi.org/10.1007/0-387-28942-9_2
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