Abstract
The theoretical and experimental background for the cloning techniques is closely tied with the biological processes described in Part 1. Volumes of protocols are available for use in gene cloning. Fortunately, the fundamentals of the techniques involved are not difficult to understand.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
Chapter 8: Techniques Used in Cloning
Cohen, S. N. 1975. The manipulation of genes. Sci. Am. 233(1), 25–33.
Martin, C., Bresnick, L., Juo, R.-R., Voyta, J. C., and Bronstein, I. 1991. Improved chemiluminescent DNA sequencing. BioTechniques 11, 110–114.
Mullis, K. B. 1990. The unusual origin of the polymerase chain reaction. Sci. Am. 262(4), 56–65.
Sanger, F. 1981. Determination of nucleotide sequences in DNA. Bioscience Reports 1, 3–18.
Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.
Thomas, P. S. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 22, 5201–5205.
Rights and permissions
Copyright information
© 2006 Springer Science+Business Media, Inc.
About this chapter
Cite this chapter
(2006). Techniques Used in Cloning. In: The ABCs of Gene Cloning. Springer, Boston, MA. https://doi.org/10.1007/0-387-28679-9_8
Download citation
DOI: https://doi.org/10.1007/0-387-28679-9_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-28663-1
Online ISBN: 978-0-387-28679-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)