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Virus Ion Channels Formed by Vpu of HIV-1, the 6K Protein of Alphaviruses and NB of Influenza B Virus

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Viral Membrane Proteins: Structure, Function, and Drug Design

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References

  • Betakova, T., Nermut, M.V., and Hay, A.J. (1996). The NB protein is an integral component of the membrane of influenza B virus. J. Gen. Virol. 77 (Pt 11), 2689–2694.

    Article  PubMed  CAS  Google Scholar 

  • Brassard, D.L., Leser, G.P., and Lamb, R.A. (1996). Influenza B virus NB glycoprotein is a component of the virion. Virology 220, 350–360.

    Article  PubMed  CAS  Google Scholar 

  • Carrasco, L. (1977). The inhibition of cell functions after viral infection. A proposed general mechanism. FEBS Lett. 76, 11–15.

    Article  PubMed  CAS  Google Scholar 

  • Carrasco, L. (1995). Modification of membrane permeability by animal viruses. Adv. Virus Res. 45, 61–112.

    PubMed  CAS  Google Scholar 

  • Carrasco, L., Perez, L., Irurzun, A., Lama, J., Martinez-Abarca, F., Rodrigez, P. et al. (1993). Modification of membrane permeability by animal viruses. L. Carrasco, N. Sonenberg, and Wimmer, E. (eds). Regulation of Gene Expression in Animal Viruses. Plenum, New York, pp. 283–305.

    Google Scholar 

  • Carrere-Kremer, S., Montpellier-Pala, C., Cocquerel, L., Wychowski, C., Penin, F., and Dubuisson, J. (2002). Subcellular localization and topology of the p7 polypeptide of hepatitis C virus. J. Virol. 76, 3720–3730.

    Article  PubMed  CAS  Google Scholar 

  • Davies, W.L., Grunert, R.R., Haft, R.F., McGahen, J.W., Neumayer, E.M., Paulshock, M. et al. (1964). Antiviral activity of 1-adamantanamine (amantadine). Science 144, 862–863.

    Article  PubMed  CAS  Google Scholar 

  • Du, B., Wolf, A., Lee, S., and Terwilliger, E. (1993). Changes in the host range and growth potential of an hiv-1 clone are conferred by the vpu gene. Virology 195, 260–264.

    Article  PubMed  CAS  Google Scholar 

  • Duff, K.C. and Ashley, R.H. (1992). The transmembrane domain of influenza A M2 protein forms amantadine-sensitive proton channels in planar lipid bilayers. Virology 190, 485–489.

    Article  PubMed  CAS  Google Scholar 

  • Emerman, M. and Malim, M.H. (1998). HIV-1 regulatory/accessory genes: Keys to unraveling viral and host cell biology. Science 280, 1880–1884.

    Article  PubMed  CAS  Google Scholar 

  • Ewart, G.D., Mills, K., Cox, G.B., and Gage, P.W. (2002). Amiloride derivatives block ion channel activity and enhancement of virus-like particle budding caused by HIV-1 protein Vpu. Eur. Biophys. J. 31, 26–35.

    Article  PubMed  CAS  Google Scholar 

  • Ewart, G.D., Nasr, N., Naif, H., Cox, G.B., Cunningham, A.L. and Gage, P.W. (2004). Potential new anti-human immunodeficiency virus type 1 compounds depress virus replication in cultured human macrophages. Antimicrobial Agents and Chemotherapy 48, 2325–2330.

    Article  PubMed  CAS  Google Scholar 

  • Ewart, G.D., Sutherland, T., Gage, P.W., and Cox, G.B. (1996). The Vpu protein of human immunodeficiency virus type 1 forms cation-selective ion channels. J. Virol. 70, 7108–7115.

    PubMed  CAS  Google Scholar 

  • Fischer, W.B., Forrest, L.R., Smith, G.R., and Sansom, M.S. (2000a). Transmembrane domains of viral ion channel proteins: A molecular dynamics simulation study. Biopolymers 53, 529–538.

    Article  PubMed  CAS  Google Scholar 

  • Fischer, W.B., Pitkeathly, M., and Sansom, M.S. (2001). Amantadine blocks channel activity of the transmembrane segment of the NB protein from influenza B. Eur. Biophys. J. 30, 416–420.

    Article  PubMed  CAS  Google Scholar 

  • Fischer, W.B., Pitkeathly, M., Wallace, B.A., Forrest, L.R., Smith, G.R., and Sansom, M.S. (2000b). Transmembrane peptide NB of influenza B: A simulation, structure, and conductance study. Biochemistry 39, 12708–12716.

    Article  PubMed  CAS  Google Scholar 

  • Fischer, W.B., and Sansom, M.S. (2002). Viral ion channels: Structure and function. Biochim. Biophys. Acta 1561, 27–45.

    Article  PubMed  CAS  Google Scholar 

  • Gaedigk-Nitschko, K., Ding, M.X., Levy, M.A., and Schlesinger, M.J. (1990). Site-directed mutations in the Sindbis virus 6K protein reveal sites for fatty acylation and the underacylated protein affects virus release and virion structure. Virology 175, 282–291.

    Article  PubMed  CAS  Google Scholar 

  • Garoff, H., Hewson, R., and Opstelten, D.J. (1998). Virus maturation by budding. pdf, hc review. 62, 1171–1190.

    CAS  Google Scholar 

  • Garoff, H. and Simons, K. (1974). Location of the spike glycoproteins in the Semliki Forest virus membrane. Proc. Natl. Acad. Sci. USA 71, 3988–3992.

    Article  PubMed  CAS  Google Scholar 

  • Gonzalez, M.E., and Carrasco, L. (1998). The human immunodeficiency virus type 1 Vpu protein enhances membrane permeability. Biochemistry 37, 13710–13719.

    Article  PubMed  CAS  Google Scholar 

  • Grice, A.L., Kerr, I.D., and Sansom, M.S. (1997). Ion channels formed by HIV-1 Vpu: A modelling and simulation study. FEBS Lett. 405, 299–304.

    Article  PubMed  CAS  Google Scholar 

  • Griffin, S.D., Beales, L.P., Clarke, D.S., Worsfold, O., Evans, S.D., Jaeger, J. et al. (2003). The p7 protein of hepatitis C virus forms an ion channel that is blocked by the antiviral drug, Amantadine. FEBS Lett. 535, 34–38.

    Article  PubMed  CAS  Google Scholar 

  • Hatta, M. and Kawaoka, Y. (2003). The NB protein of influenza B virus is not necessary for virus replication in vitro. J. Virol. 77, 6050–6054.

    Article  PubMed  CAS  Google Scholar 

  • Kerkau, T., Bacik, I., Bennink, J.R., Yewdell, J.W., Hunig, T., Schimpl, A. et al. (1997). The human immunodeficiency virus type 1 (HIV-1) Vpu protein interferes with an early step in the biosynthesis of major histocompatibility complex (MHC) class I molecules. J. Exp. Med. 185, 1295–1305.

    Article  PubMed  CAS  Google Scholar 

  • Lama, J. and Carrasco, L. (1992). Expression of poliovirus nonstructural proteins in Escherichia coli cells. Modification of membrane permeability induced by 2B and 3A. J. Biol. Chem. 267, 15932–15937.

    PubMed  CAS  Google Scholar 

  • Liljestrom, P., Lusa, S., Huylebroeck, D., and Garoff, H. (1991). In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: The small 6,000-molecular-weight membrane protein modulates virus release. J. Virol. 65, 4107–4113.

    PubMed  CAS  Google Scholar 

  • Loewy, A., Smyth, J., von Bonsdorff, C.H., Liljestrom, P., and Schlesinger, M.J. (1995). The 6-kilodalton membrane protein of Semliki Forest virus is involved in the budding process. J. Virol. 69, 469–475.

    PubMed  CAS  Google Scholar 

  • Lusa, S., Garoff, H., and Liljestrom, P. (1991). Fate of the 6K membrane protein of Semliki Forest virus during virus assembly. Virology 185, 843–846.

    Article  PubMed  CAS  Google Scholar 

  • Melton, J.V., Ewart, G.D., Weir, R.C., Board, P.G., Lee, E., and Gage, P.W. (2002). Alphavirus 6K proteins form ion channels. J. Biol. Chem. 277, 46923–46931.

    Article  PubMed  CAS  Google Scholar 

  • Miller, C. (1986). Ion Channel Reconstitution, 1st edn. Plenum Press, New York and London.

    Google Scholar 

  • Montal, M. (2003). Structure-function correlates of Vpu, a membrane protein of HIV-1. FEBS Lett. 552, 47–53.

    Article  PubMed  CAS  Google Scholar 

  • Moore, P.B., Zhong, Q., Husslein, T., and Klein, M.L. (1998). Simulation of the HIV-1 Vpu Transmembrane domain as a pentameric bundle. FEBS Lett. 431, 143–148.

    Article  PubMed  CAS  Google Scholar 

  • Mosier, D. and Sieburg, H. (1994). Macrophage-tropic HIV: Critical for AIDS pathogenesis? [see comments]. Immunol. Today. 15, 332–339.

    Article  PubMed  CAS  Google Scholar 

  • Mould, J.A., Drury, J.E., Frings, S.M., Kaupp, U.B., Pekosz, A., Lamb, R.A. et al. (2000). Permeation and activation of the M2 ion channel of influenza A virus. J. Biol. Chem. 275, 31038–31050.

    Article  PubMed  CAS  Google Scholar 

  • Munoz, A., Castrillo, J.L., and Carrasco, L. (1985). Modification of membrane permeability during Semliki Forest virus infection. Virology 146, 203–212.

    Article  PubMed  CAS  Google Scholar 

  • Pavlovic, D., Neville, D.C., Argaud, O., Blumberg, B., Dwek, R.A., Fischer, W.B. et al. (2003). The hepatitis C virus p7 protein forms an ion channel that is inhibited by long-alkyl-chain iminosugar derivatives. Proc. Natl. Acad. Sci. USA 100, 6104–6108.

    Article  PubMed  CAS  Google Scholar 

  • Pekosz, A. and Lamb, R.A. (1997). The CM2 protein of influenza C virus is an oligomeric integral membrane glycoprotein structurally analogous to influenza A virus M2 and influenza B virus NB proteins. Virology 237, 439–451.

    Article  PubMed  CAS  Google Scholar 

  • Piller, S.C., Ewart, G.D., Premkumar, A., Cox, G.B., and Gage, P.W. (1996). Vpr protein of human immunodeficiency virus type 1 forms cation-selective channels in planar lipid bilayers. Proc. Natl. Acad. Sci. USA 93, 111–115.

    Article  PubMed  CAS  Google Scholar 

  • Pinto, L.H., Holsinger, L.J., and Lamb, R.A. (1992). Influenza virus M2 protein has ion channel activity. Cell 69, 517–528.

    Article  PubMed  CAS  Google Scholar 

  • Plugge, B., Gazzarrini, S., Nelson, M., Cerana, R., Van Etten, J.L., Derst, C. et al. (2000). A potassium channel protein encoded by chlorella virus PBCV-1. Science 287, 1641–1644.

    Article  PubMed  CAS  Google Scholar 

  • Porter, A.G. (1993). Picornavirus nonstructural proteins: Emerging roles in virus replication and inhibition of host cell functions. J. Virol. 67, 6917–6921.

    PubMed  CAS  Google Scholar 

  • Sanz, M.A. and Carrasco, L. (2001). Sindbis virus variant with a deletion in the 6K gene shows defects in glycoprotein processing and trafficking: Lack of complementation by a wild-type 6K gene in trans. J. Virol. 75, 7778–7784.

    Article  PubMed  CAS  Google Scholar 

  • Schlesinger, M.J., London, S.D., and Ryan, C. (1993). An in-frame insertion into the Sindbis virus 6K gene leads to defective proteolytic processing of the virus glycoproteins, a trans-dominant negative inhibition of normal virus formation, and interference in virus shut off of host-cell protein synthesis. Virology 193, 424–432.

    Article  PubMed  CAS  Google Scholar 

  • Schubert, U., Anton, L.C., Bacik, I., Cox, J.H., Bour, S., Bennink, J.R. et al. (1998). CD4 glycoprotein degradation induced by human immunodeficiency virus type 1 Vpu protein requires the function of proteasomes and the ubiquitin-conjugating pathway. J. Virol. 72, 2280–2288.

    PubMed  CAS  Google Scholar 

  • Schubert, U., Bour, S., Ferrer-Montiel, A.V., Montal, M., Maldarell, F., and Strebel, K. (1996a). The two biological activities of human immunodeficiency virus type 1 Vpu protein involve two separable structural domains. J. Virol. 70, 809–819.

    PubMed  CAS  Google Scholar 

  • Schubert, U., Bour, S., Willey, R.L., and Strebel, K. (1999). Regulation of virus release by the macrophage-tropic human immunodeficiency virus type 1 AD8 isolate is redundant and can be controlled by either Vpu or Env. J. Virol. 73, 887–896.

    PubMed  CAS  Google Scholar 

  • Schubert, U., Ferrer-Montiel, A.V., Oblatt-Montal, M., Henklein, P., Strebel, K., and Montal, M. (1996b). Identification of an ion channel activity of the Vpu transmembrane domain and its involvement in the regulation of virus release from HIV-1-infected cells. FEBS Lett. 398, 12–18.

    Article  PubMed  CAS  Google Scholar 

  • Schuitemaker, H. (1994). Macrophage-tropic HIV-1 variants: Initiators of infection and AIDS pathogenesis? J. Leukoc. Biol. 56, 218–224.

    PubMed  CAS  Google Scholar 

  • Shaw, M.W. and Choppin, P. W. (1984). Studies on the synthesis of the influenza V virus NB glycoprotein. Virology 139, 178–184.

    Article  PubMed  CAS  Google Scholar 

  • Shaw, M.W., Choppin, P.W., and Lamb, R.A. (1983). A previously unrecognized influenza B virus glycoprotein from a bicistronic mRNA that also encodes the viral neuraminidase. Proc. Natl. Acad. Sci. USA 80, 4879–4883.

    Article  PubMed  CAS  Google Scholar 

  • Sonnhammer, E.L., von Heijne, G., and Krogh, A. (1998). A hidden Markov model for predicting transmembrane helices in protein sequences. Proc. Int. Conf. Intell. Syst. Mol. Biol. 6, 175–182.

    PubMed  CAS  Google Scholar 

  • Sunstrom, N.A., Premkumar, L.S., Premkumar, A., Ewart, G., Cox, G.B., and Gage, P.W. (1996). Ion channels formed by NB, an influenza B virus protein. J. Membr. Biol. 150, 127–132.

    Article  PubMed  CAS  Google Scholar 

  • Tosteson, M.T., Pinto, L.H., Holsinger, L.J., and Lamb, R.A. (1994). Reconstitution of the influenza virus M2 ion channel in lipid bilayers. J. Membr. Biol. 142, 117–126.

    PubMed  CAS  Google Scholar 

  • Ulug, E.T., Garry, R.F., Waite, M.R., and Bose, H.R., Jr. (1984). Alterations in monovalent cation transport in Sindbis virus-infected chick cells. Virology 132, 118–130.

    Article  PubMed  CAS  Google Scholar 

  • van Kuppeveld, F.J., Hoenderop, J.G., Smeets, R.L., Willems, P.H., Dijkman, H.B., Galama, J.M. et al. (1997). Coxsackievirus protein 2B modifies endoplasmic reticulum membrane and plasma membrane permeability and facilitates virus release. EMBO J. 16, 3519–3532.

    Article  PubMed  Google Scholar 

  • von Heijne, G. (1988). Transcending the impenetrable: How proteins come to terms with membranes. Biochim. Biophys. Acta 947, 307–333.

    Google Scholar 

  • Willbold, D., Hoffmann, S., and Rosch, P. (1997). Secondary structure and tertiary fold of the human immunodeficiency virus protein U (Vpu) cytoplasmic domain in solution. Eur. J. Biochem. 245, 581–588.

    Article  PubMed  CAS  Google Scholar 

  • Williams, M.A. and Lamb, R.A. (1986). Determination of the orientation of an integral membrane protein and sites of glycosylation by oligonucleotide-directed mutagenesis: Influenza B virus NB glycoprotein lacks a cleavable signal sequence and has an extracellular NH2-terminal region. Mol. Cell. Biol. 6, 4317–4328.

    PubMed  CAS  Google Scholar 

  • Williams, M.A. and Lamb, R.A. (1988). Polylactosaminoglycan modification of a small integral membrane glycoprotein, influenza B virus NB. Mol. Cell. Biol. 8, 1186–1196.

    PubMed  CAS  Google Scholar 

  • Wray, V., Kinder, R., Federau, T., Henklein, P., Bechinger, B., and Schubert, U. (1999). Solution structure and orientation of the transmembrane anchor domain of the HIV-1-encoded virus protein U by high-resolution and solid-state NMR spectroscopy. Biochemistry 38, 5272–5282.

    Article  PubMed  CAS  Google Scholar 

  • Zhirnov, O.P. (1990). Solubilization of matrix protein M1/M from virions occurs at different pH for orthomyxo-and paramyxoviruses. Virology 176, 274–279.

    Article  PubMed  CAS  Google Scholar 

  • Zhirnov, O.P. (1992). Isolation of matrix protein M1 from influenza viruses by acid-dependent extraction with nonionic detergent. Virology 186, 324–330.

    Article  PubMed  CAS  Google Scholar 

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Gage, P.W., Ewart, G., Melton, J., Premkumar, A. (2005). Virus Ion Channels Formed by Vpu of HIV-1, the 6K Protein of Alphaviruses and NB of Influenza B Virus. In: Fischer, W.B. (eds) Viral Membrane Proteins: Structure, Function, and Drug Design. Protein Reviews, vol 1. Springer, Boston, MA. https://doi.org/10.1007/0-387-28146-0_15

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