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DNA Methylation and Cancer Therapy pp 1–12Cite as

DNA Methylation

Three Decades in Search of Function

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Part of the book series: Medical Intelligence Unit ((MIUN))

Abstract

DNA methylation is an epigenetic mark that is involved in control mechanisms of a variety of biological processes. Being symmetrically positioned on the two comple-mentary DNA strands the methyl groups represent a clonally inheritable feature of the DNA. Once established during embryogenesis, methylation patterns are maintained for many cell generations by a maintenance methyltransferase. These methylation patterns are interpreted by proteins that interact with the DNA depending on its state of methylation. Since methylation patterns provide a universal code for DNA-protein interactions, it is not surprising that methylation takes part in many biological processes such as: control of gene expression, DNA replication and cell cycle, DNA repair, imprinting, inactivation of the X-chromosome in eutherian females and much more. In essence, DNA methylation patterns fulfill their task by guiding specific proteins to target sites on the DNA.

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References

  1. Razin A, Sedat JW, Sinsheimer RL. Structure of the DNA of bacteriophage ϕX174. VII. Methylation. J Mol Biol 1970; 53:251–259.

    PubMed  CAS  Google Scholar 

  2. Razin A, Sedat JW, Sinsheimer RL. In vivo methylation of replicating bacteriophage ϕX174 DNA. J Mol Biol 1973; 78:417–425.

    PubMed  CAS  Google Scholar 

  3. Razin A. DNA methylase induced by bacteriophage (ϕX174. Proc Natl Acad Sci USA 1973; 70:3773–3775.

    PubMed  CAS  Google Scholar 

  4. Friedman J, Razin A. Studies on the biological role of DNA methylation; II. Role of ϕX174 DNA methylation in the process of viral progeny DNA synthesis. Nucleic Acids Res 1976; 3:2665–2675.

    PubMed  CAS  Google Scholar 

  5. Friedman J, Friedmann A, Razin A. Studies on the biological role of DNA methylation: III Role in excision of one-genome long single-stranded ϕX174 DNA. Nucleic Acids Res 1977; 4:3483–3496.

    PubMed  CAS  Google Scholar 

  6. Fujisawa H, Hayashi M. Gene A product of ϕX174 is required for site-specific endonucleolytic cleavage during single-stranded DNA synthesis in vivo. J Virol 1976; 19:416–424.

    PubMed  CAS  Google Scholar 

  7. Kuhnlein U, Arber W. Host specificity of DNA produced by Escherichia coli. XV. The role of nucleotide methylation in in vitro B-specific modification. J Mol Biol 1972; 63:9–19.

    PubMed  CAS  Google Scholar 

  8. Radman M, Wagner R. Mismatch repair in Escherichia coli. Annu Rev Genet 1986; 20:523–538.

    PubMed  CAS  Google Scholar 

  9. Razin A, Urieli S, Pollack Y et al. Studies on the biological role of DNA methylation; IV. Mode of methylation of DNA in E. coli cells. Nucleic Acids Res 1980; 8:1783–1792.

    PubMed  CAS  Google Scholar 

  10. Szyf M, Gruenbaum Y, Urieli-Shoval S et al. Studies on the biological role of DNA methylation: V. The pattern of E. coli DNA methylation. Nucleic Acids Res 1982; 10:7247–7259.

    PubMed  CAS  Google Scholar 

  11. Ogden GB, Pratt MJ, Schaechter M. The replicative origin of the E. coli chromosome binds to cell membranes only when hemimethylated. Cell 1988; 54:127–135.

    PubMed  CAS  Google Scholar 

  12. Campbell JL, Kleckner NE. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork. Cell 1990; 62:967–979.

    PubMed  CAS  Google Scholar 

  13. Roberts D, Hoopes BC, McClure WR et al. IS10 transposition is regulated by DNA adenine methylation. Cell 1985; 43:117–130.

    PubMed  CAS  Google Scholar 

  14. Scarano E. The control of gene function in cell differentiation and in embryogenesis. Adv Cytopharmacol 1971; 1:13–24.

    PubMed  CAS  Google Scholar 

  15. Holliday R, Pugh JE. DNA modification mechanisms and gene activity during development. Science 1975; 187:226–232.

    PubMed  CAS  Google Scholar 

  16. Riggs AD. X inactivation, differentiation, and DNA methylation. Cytogenet. Cell Genet 1975; 14:9–25.

    CAS  Google Scholar 

  17. Naveh-Many T, Cedar H. Active gene sequences are undermethylated. Proc Natl Acad Sci USA 1981; 78:4246–4250.

    PubMed  CAS  Google Scholar 

  18. Stein R, Sciaky-Gallili N, Razin A et al. Pattern of methylation of two genes coding for house-keeping functions. Proc Natl Acad Sci USA 1983; 80:2422–2426.

    PubMed  CAS  Google Scholar 

  19. Yisraeli J, Szyf M. Gene methylation patterns and expression. In: Razin A, Cedar H, Riggs AD, eds. DNA methylation: Biochemistry and Biological Significance. New York: Springer-Verlag, 1984:352–370.

    Google Scholar 

  20. Yeivin A, Razin A. Gene methylation patterns and expression. In: Jost JP, Saluz HP, eds. DNA Methylation: Molecular Biology and Biological Significance. Basel: Birkhauser Verlag, 1993:523–568.

    Google Scholar 

  21. Razin A, Riggs AD. DNA methylation and gene function. Science 1980; 210:604–610.

    PubMed  CAS  Google Scholar 

  22. Pollack Y, Stein R, Razin A et al. Methylation of foreign DNA sequences in eukaryotic cells. Proc Natl Acad Sci USA 1980; 77:6463–6467.

    PubMed  CAS  Google Scholar 

  23. Wigler M, Levy D, Perucho M. The somatic replication of DNA methylation. Cell 1981; 24:33–40.

    PubMed  CAS  Google Scholar 

  24. Stein R, Gruenbaum Y, Pollack Y et al. Clonal inheritance of the pattern DNA methylation in mouse cells. Proc Natl Acad Sci USA 1982; 79:61–65.

    PubMed  CAS  Google Scholar 

  25. Gruenbaum Y, Cedar H, Razin A. Substrate and sequence specificity of a eukaryotic DNA methylase. Nature 1982; 292:620–622.

    Google Scholar 

  26. Bestor TH, Ingram VM. Two DNA methyltransferases from murine erythroleukemia cells: Purification, sequence specificity, and mode of interaction with DNA. Proc Natl Acad Sci USA 1983; 80:5559–5563.

    PubMed  CAS  Google Scholar 

  27. Gruenbaum Y, Szyf M, Cedar H et al. Methylation of replicating and post-replicated mouse L-cell DNA. Proc Natl Acad Sci USA 1983; 80:4919–4921.

    PubMed  CAS  Google Scholar 

  28. Stein R, Razin A, Cedar H. In vitro methylation of the hamster APRT gene inhibits its expression in mouse L-cells. Proc Natl Acad Sci USA 1982; 79:3418–3422.

    PubMed  CAS  Google Scholar 

  29. Cedar H, Stein R, Gruenbaum Y et al. Effect of DNA Methylation on Gene Expression. Cold Spring Harbor Symposia on Quantitative Biology, 1983:605–609.

    Google Scholar 

  30. Stewart CL, Stuhlmann H, Jahner D et al. De novo methylation, expression, and infectivity of retroviral genomes introduced into embryonal carcinoma cells. Proc Natl Acad Sci USA 1982; 79:4098–4102.

    PubMed  CAS  Google Scholar 

  31. Jones PA. Gene activation by 5-azacytidine. In: Razin A, Cedar H, Riggs AD, eds. DNA Methylation: Biochemistry and Biological Significance. New York: Springer-Verlag, 1984:165–188.

    Google Scholar 

  32. Mohandas T, Sparker RS, Shapiro LJ. Reactivation of an inactive human X chromosome: Evidence for X inactivation by DNA methylation. Science 1981; 211:393–396.

    PubMed  CAS  Google Scholar 

  33. Jones PA, Taylor SM, Mohandas T et al. Cell cycle-specific reactivation of an inactive X-chromosome locus by 5-azadeoxycytidine. Proc Natl Acad Sci USA 1982; 79:1215–1219.

    PubMed  CAS  Google Scholar 

  34. Razin A, Cedar H, Riggs AD, eds. DNA methylation: Biochemistry and Biological Significance. New York: Springer Verlag, 1984.

    Google Scholar 

  35. Kafri T, Ariel M, Brandeis M et al. Developmental pattern of gene-specific DNA methylation in the mouse embryo and germline. Genes Dev 1992; 6:705–714.

    PubMed  CAS  Google Scholar 

  36. Monk M, Boubelik M, Lehnert S. Temporal and regional changes in DNA methylation in the embryonic, extraembryonic and germ cell lineages during mouse embryo development. Development 1987; 99:371–382.

    PubMed  CAS  Google Scholar 

  37. Okano M, Xie S, Li E. Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases. Nature Genet 1998; 19:219–20.

    PubMed  CAS  Google Scholar 

  38. Okano M, Bell DW, Haber DA et al. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 1999; 99:247–57.

    PubMed  CAS  Google Scholar 

  39. Bird AP. CpG-rich islands and the function of DNA methylation. Nature 1986; 321:209–213.

    PubMed  CAS  Google Scholar 

  40. Frank D, Keshet I, Shani M et al. Demethylation of CpG islands in embryonic cells. Nature 1991; 351:239–241.

    PubMed  CAS  Google Scholar 

  41. Brandeis M, Frank D, Keshet I et al. Sp1 elements protect a CpG island from de novo methylation. Nature 1994; 371:435–438.

    PubMed  CAS  Google Scholar 

  42. Macleod D, Charlton J, Mullins J et al. Sp1 sites in the mouse Aprt gene promoter are required to prevent methylation of the CpG island. Genes Dev 1994; 8:2282–2292.

    PubMed  CAS  Google Scholar 

  43. Benvenisty N, Szyf M, Mencher D et al. Tissue-specific hypomethylation and expression of rat phosphoenolpyruvate carboxykinase gene induced by in vivo treatment of fetuses and neonates with 5-azacytidine. Biochemistry 1985; 24:5015–5019.

    PubMed  CAS  Google Scholar 

  44. Benvenisty N, Mencher D, Meyuchas O et al. Sequential changes in DNA methylation patterns of the rat phosphoenolpyruvate carboxykinase gene during development. Proc Natl Acad Sci USA 1985; 82:267–271.

    PubMed  CAS  Google Scholar 

  45. Kafri T, Gao X, Razin A. Mechanistic aspects of genome-wide demethylation in the preimplantation mouse embryo. Proc Natl Acad Sci USA 1993; 90:10558–10562.

    PubMed  CAS  Google Scholar 

  46. Szyf M, Eliasson L, Mann V et al. Cellular and viral DNA hypomethylation associated with induction of Epstein-Barr virus lytic cycle. Proc Natl Acad Sci USA 1985; 82:8090–8094.

    PubMed  CAS  Google Scholar 

  47. Sullivan CH, Grainger RM. δ-Crystallin genes become hypomethylated in postmitotic lens cells during chicken development. Proc Natl Acad Sci USA 1986; 83:329–333.

    Google Scholar 

  48. Paroush Z, Keshet I, Yisraeli J et al. Dynamics of demethylation and activation of the α actin gene in myoblasts. Cell 1990; 63:1229–1237.

    PubMed  CAS  Google Scholar 

  49. Jost JP, Seldran M. Association of transcriptionally active vitellogenin II gene with the nuclear matrix of chicken liver. EMBO J 1984; 3:2005–2008.

    PubMed  CAS  Google Scholar 

  50. Frank D, Mintzer-Lichenstein M, Paroush Z et al. Demethylation of genes in animal cells. Philo Trans Royal Soc 1990; 326:241–251.

    CAS  Google Scholar 

  51. Razin A, Szyf M, Kafri T et al. Replacement or 5-methylcytosine by cytosine: A possible mechanism for transient DNA demethylation during differentiation. Proc Natl Acad Sci USA 1986; 83:2827–2831.

    PubMed  CAS  Google Scholar 

  52. Jost JP. Nuclear extracts of chicken embryos promote an active demethylation of DNA by excision repair of 5-methyldeoxycytidine. Proc Natl Acad Sci USA 1993; 90:4684–4688.

    PubMed  CAS  Google Scholar 

  53. Vairapandi M, Duker N. Enzymic removal of 5-methylcytosine from DNA by a human DNA-glycosylase. Nucleic Acids Res 1993; 21:5323–5327.

    PubMed  CAS  Google Scholar 

  54. Fremont M, Siegmann M, Gaulis S et al. Demethylation of DNA by purified chick embryo 5-methylcytosine-DNA glycosylase requires both protein and RNA. Nucleic Acids Res 1997; 25:2375–2380.

    PubMed  CAS  Google Scholar 

  55. Jost JP, Oakeley EJ, Zhu B et al. 5-Methylcytosine DNA glycosylase participates in the genome-wide loss of DNA methylation occurring during mouse myoblast differentiation. Nucleic Acids Res 2001; 29:4452–4461.

    PubMed  CAS  Google Scholar 

  56. Weiss A, Keshet I, Razin A et al. DNA demethylation in vitro: Involvement of RNA. Cell 1996; 86:709–718.

    PubMed  CAS  Google Scholar 

  57. Bhattacharya SK, Ramchandani S, Cervoni N et al. A mammalian protein with specific demethylase activity for mCpG DNA. Nature 1999; 397:579–583.

    PubMed  CAS  Google Scholar 

  58. Cervoni N, Bhattacharya S, Szyf M. DNA demethylase is a processive enzyme. J Biol Chem 1999; 274:8363–8366.

    PubMed  CAS  Google Scholar 

  59. Bestor TH. Cloning of a mammalian DNA methyltransferase. Gene 1988; 74:9–12.

    PubMed  CAS  Google Scholar 

  60. Li E, Bestor TH, Jaenisch R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 1992; 69:915–926.

    PubMed  CAS  Google Scholar 

  61. Beard C, Li E, Jaenisch R. Loss of methylation activates Xist in somatic but not in embryonic cells. Genes Dev 1995; 9:2325–2334.

    PubMed  CAS  Google Scholar 

  62. Razin A, Cedar H. DNA methylation and genomic imprinting. Cell 1994; 77:473–476.

    PubMed  CAS  Google Scholar 

  63. Li E, Beard C, Jaenisch R. Role for DNA methylation in genomic imprinting. Nature 1993; 366:362–365.

    PubMed  CAS  Google Scholar 

  64. Birger Y, Shemer R, Perk J et al. The imprinting box of the mouse Igf2r gene. Nature 1999; 397:84–88.

    PubMed  CAS  Google Scholar 

  65. Shemer R, Hershko AY, Perk J et al. The imprinting box of the Prader-Willi/Angelman Syndrome domain. Nature Genet 2000; 26:440–443.

    PubMed  CAS  Google Scholar 

  66. Bartolomei MS, Webber AL, Brunkow ME et al. Epigenetic mechanisms underlying the imprinting of the mouse H19 gene. Genes Dev 1993; 7:1663–1673.

    PubMed  CAS  Google Scholar 

  67. Tucker KL, Beard C, Dausman J et al. Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not non imprinted genes. Genes Dev 1996; 10:1008–1020.

    PubMed  CAS  Google Scholar 

  68. Shemer R, Birger Y, Riggs AD et al. Structure of the imprinted mouse Snrpn gene and establishment of its parental-specific methylation pattern. Proc Natl Acad Sci USA 1997; 94:10267–10272.

    PubMed  CAS  Google Scholar 

  69. Razin A, Cedar H. Distribution of 5-methylcytosine in chromatin. Proc Natl Acad Sci USA 1977; 74:2725–2728.

    PubMed  CAS  Google Scholar 

  70. Weintraub H, Groudine M. Chromosomal subunits in active genes have an altered conformation. Science 1976; 193:848–856.

    PubMed  CAS  Google Scholar 

  71. Shemer R, Razin A. Establishment of imprinted methylation patterns during development. In: Russo VEA, Martienssen RA, Riggs AD, eds. Epigenetic Mechanisms of Gene Regulation: Cold Spring Harbor Laboratory Press, 1996:215–229.

    Google Scholar 

  72. Migeon BR. X-chromosome inactivation: Molecular mechanisms and genetic consequences. Trends Genet 1994; 10:230–235.

    PubMed  CAS  Google Scholar 

  73. Yoder JA, Walsh CP, Bestor TH. Cytosine methylation and the ecology of intragenomic parasites. Trends Genet 1997; 13:335–340.

    PubMed  CAS  Google Scholar 

  74. Levine A, Yeivin A, Ben-Asher E et al. Histone H1 mediated inhibition of transcription initiation of methylated templates in vitro. J Biol Chem 1993; 268:21754–21759.

    PubMed  CAS  Google Scholar 

  75. Barra JL, Rhounim L, Rossignol JL et al. Histone H1 is dispensable for methylation-associated gene silencing in Ascobolus immersus and essential for long life span. Mol Cell Biol 2000; 20:61–69.

    PubMed  CAS  Google Scholar 

  76. Razin A. CpG methylation, chromatin structure and gene silencing—a three-way connection. EMBO J 1998; 17:4905–4908.

    PubMed  CAS  Google Scholar 

  77. Meehan RR, Lewis JD, McKay S et al. Identification of a mammalian protein that binds specifically to DNA containing methylated CpGs. Cell 1989; 58:499–507.

    PubMed  CAS  Google Scholar 

  78. Lewis JD, Meehan RR, Henzel WJ, et al. Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA. Cell 1992; 69:905–14.

    PubMed  CAS  Google Scholar 

  79. Boyes J, Bird A. DNA methylation inhibits transcription indirectly via a methyl-CpG biding protein. Cell 1991; 64:1123–1134.

    PubMed  CAS  Google Scholar 

  80. Boyes J, Bird A. Repression of genes by DNA methylation depends on CpG density and promoter strength; evidence for involvement of a methyl-CpG binding protein. EMBO J 1992; 11:327–333.

    PubMed  CAS  Google Scholar 

  81. Levine A, Cantoni GL, Razin A. Inhibition of promoter activity by methylation: Possible involvement of protein mediators. Proc Natl Acad Sci USA 1991; 88:6515–6518.

    PubMed  CAS  Google Scholar 

  82. Levine A, Cantoni GL, Razin A. Methylation in the preinitiation domain suppresses gene transcription by an indirect mechanism. Proc Natl Acad Sci USA 1992; 89:10119–10123.

    PubMed  CAS  Google Scholar 

  83. Nan X, Meehan RR, Bird A. Dissection of the methyl-CpG binding domain from the chromosomal protein MeCP2. Nucleic Acids Res 1993; 21:4886–4892.

    PubMed  CAS  Google Scholar 

  84. Nan X, Tate P, Li E et al. DNA methylation specifies chromosomal localization of MeCP2. Mol Cell Biol 1996; 16:414–421.

    PubMed  CAS  Google Scholar 

  85. Nan X, Campoy FJ, Bird A. MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin. Cell 1997; 88:471–481.

    PubMed  CAS  Google Scholar 

  86. Nan X, Ng H-H, Johnson CA et al. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature 1998; 393:386–389.

    PubMed  CAS  Google Scholar 

  87. Jones PL, Veenstra GJC, Wade PA et al. Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription. Nature Genet 1998; 19:187–191.

    PubMed  CAS  Google Scholar 

  88. Eden S, Hashimshony T, Keshet I et al. DNA methylation models histone acetylation. Nature 1998; 394:842–843.

    PubMed  CAS  Google Scholar 

  89. Hendrich B, Bird A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol 1998; 18:6538–6547.

    PubMed  CAS  Google Scholar 

  90. Ng HH, Zhang Y, Hendrich B et al. MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. Nature Genet 1999; 23:58–61.

    PubMed  CAS  Google Scholar 

  91. Feng Q, Zhang Y. The MeCP1 complex represses transcription through preferential binding, remodeling, and deacetylating methylated nucleosomes. Genes Dev 2001; 15:827–832.

    PubMed  CAS  Google Scholar 

  92. Zhang Y, Ng HH, Erdjument-Bromage H et al. Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation. Genes Dev 1999; 13:1924–1935.

    PubMed  CAS  Google Scholar 

  93. Hendrich B, Guy J, Ramsahoye B et al. Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development. Genes Dev 2001; 15:710–723.

    PubMed  CAS  Google Scholar 

  94. Fuks F, Burgers WA, Godin N et al. Dnmt3a binds deacetylases and is recruited by a sequence-specific repressor to silence transcription. EMBO J 2001; 20:2536–2544.

    PubMed  CAS  Google Scholar 

  95. Robertson KD, Ait-Si-Ali S, Yokochi T et al. DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters. Nature Genet 2000; 25:338–342.

    PubMed  CAS  Google Scholar 

  96. Rountree MR, Bachman KE, Baylin SB. DNMT1 binds HDAC2 and a new corepressor, DMAP1, to form a complex at replication foci. Nature Genet 2000; 25:269–277.

    PubMed  CAS  Google Scholar 

  97. Fujita N, Takebayashi S, Okumura K et al. Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms. Mol Cell Biol 1999; 19:6415–6426.

    PubMed  CAS  Google Scholar 

  98. Ng HH, Jeppesen P, Bird A. Active repression of methylated genes by the chromosomal protein MBD1. Mol Cell Biol 2000; 20:1394–406.

    PubMed  CAS  Google Scholar 

  99. Kantor B, Razin A. DNA methylation, histone deacetylase repressory complexes and development. Gene Func Dis 2001; 2:69–75.

    CAS  Google Scholar 

  100. Goto K, Numata M, Komura JI et al. Expression of DNA methyltransferase gene in mature and immature neurons as well as proliferating cells in mice. Differentiation 1994; 56:39–44.

    PubMed  CAS  Google Scholar 

  101. Endres M, Meisel A, Biniszkiewicz D et al. DNA methyltransferase contributes to delayed ischemic brain injury. J Neurosci 2000; 20:3175–3181.

    PubMed  CAS  Google Scholar 

  102. Gibbons RJ, McDowell TL, Raman S et al. Mutations in ATRX, encoding a SWI/SNF-like protein, cause diverse changes in the pattern of DNA methylation. Nature Genet 2000; 24:368–371.

    PubMed  CAS  Google Scholar 

  103. Hansen RS, Wijmenga C, Luo P et al. The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome. Proc Natl Acad Sci USA 1999; 96:14412–14417.

    PubMed  CAS  Google Scholar 

  104. Xu GL, Bestor TH, Bourc’his D et al. Chromosome instability and immunodeficiency syndrome caused by mutations in a DNA methyltransferase gene. Nature 1999; 402:187–91.

    PubMed  CAS  Google Scholar 

  105. Tagarro I, Fernandez-Peralta AM, Gonzalez-Aguilera JJ. Chromosomal localization of human satellites 2 and 3 by a FISH method using oligonucleotides as probes. Hum Genet 1994; 93:383–388.

    PubMed  CAS  Google Scholar 

  106. Jeanpierre M, Turleau C, Aurias A et al. An embryonic-like methylation pattern of classical satellite DNA is observed in ICF syndrome. Hum Mol Genet 1993; 2:731–735.

    PubMed  CAS  Google Scholar 

  107. Kondo T, Bobek MP, Kuick R et al. Whole-genome methylation scan in ICF syndrome: Hypomethylation of non satellite DNA repeats D4Z4 and NBL2. Hum Mol Genet 2000; 9:597–604.

    PubMed  CAS  Google Scholar 

  108. Bourc’his D, Miniou P, Jeanpierre M et al. Abnormal methylation does not prevent X inactivation in ICF patients. Cytogenet Cell Genet 1999; 84:245–252.

    PubMed  CAS  Google Scholar 

  109. Miniou P, Jeanpierre M, Blanquet V et al. Abnormal methylation pattern in constitutive and facultative (X inactive chromosome) heterochromatin of ICF patients. Hum Mol Genet 1994; 3:2093–2102.

    PubMed  CAS  Google Scholar 

  110. Amir RE, Van den Veyver IB, Wan M et al. Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nature Genet 1999; 23:185–188.

    PubMed  CAS  Google Scholar 

  111. Oberle I, Rousseau F, Heitz D et al. Instability of a 550-base pair DNA segment and abnormal methylation in fragile X syndrome. Science 1991; 252:1097–1102.

    PubMed  CAS  Google Scholar 

  112. Coffee B, Zhang F, Warren ST et al. Acetylated histones are associated with FMR1 in normal but not fragile X-syndrome cells. Nature Genet 1999; 22:98–101.

    PubMed  CAS  Google Scholar 

  113. Buiting K, Saitoh S, Gross S et al. Inherited microdeletions in the Angelman and Prader-Willi syndromes define an imprinting centre on human chromosome 15. Nature Genet 1995; 9:395–400.

    PubMed  CAS  Google Scholar 

  114. Reis A, Dittrich B, Greger V et al. Imprinting mutations suggested by abnormal DNA methylation patterns in familial Angelman and Prader-Willi syndromes. Am J Hum Genet 1994; 54:741–747.

    PubMed  CAS  Google Scholar 

  115. Perk J, Makedonsky K, Lande L et al. On the imprinting mechanism of the Prader Willi/Angelman regional control center. EMBO J 2002; 21:5807–5814.

    PubMed  CAS  Google Scholar 

  116. Brown KW, Villar AJ, Bickmore W et al. Imprinting mutation in the Beckwith-Wiedemann syndrome leads to biallelic IGF2 expression through an H19-independent pathway. Hum Mol Genet 1996; 5:2027–32.

    PubMed  CAS  Google Scholar 

  117. Smilinich NJ, Day CD, Fitzpatrick GV et al. A maternally methylated CpG island in KvLQT1 is associated with an antisense paternal transcript and loss of imprinting in Beckwith-Wiedemann syndrome. Proc Nad Acad Sci USA 1999; 96:8064–8069.

    CAS  Google Scholar 

  118. Paulsen M, El-Maarri O, Engemann S et al. Sequence conservation and variability of imprinting in the Beckwith-Wiedemann syndrome gene cluster in human and mouse. Hum Mol Genet 2000; 9:1829–1841.

    PubMed  CAS  Google Scholar 

  119. Marinus MG. Methylation of prokaryotic DNA. In: Razin A, Cedar H, Riggs AD, eds. DNA methylation: Biochemistry and Biological Significance. New York: Springer Verlag, 1984:81–109.

    Google Scholar 

  120. Proffitt JH, Davie JR, Swinton D et al. 5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA. Mol Cell Biol 1984; 4:985–988.

    PubMed  CAS  Google Scholar 

  121. Urieli-Shoval S, Gruenbaum Y, Sedat J et al. The absence of detectable methylated bases in Drosophila melanogaster DNA. FEBS Lett 1982; 146:148–52.

    PubMed  CAS  Google Scholar 

  122. Simpson VJ, Johnson TE, Hammen RF. Caenorhabditis elegans DNA does not contain 5-methylcytosine at any time during development or aging. Nucleic Acids Res 1986; 14:6711–6719.

    PubMed  CAS  Google Scholar 

  123. Vogelauer M, Wu J, Suka N et al. Global histone acetylation and deacetylation in yeast. Nature 2000; 408:495–498.

    PubMed  CAS  Google Scholar 

  124. Selker EU. Trichostatin A causes selective loss of DNA methylation in Neurospora. Proc Natl Acad Sci USA 1998; 95:9430–9435.

    PubMed  CAS  Google Scholar 

  125. Cervoni N, Szyf M. Demethylase activity is directed by histone acetylation. J Biol Chem 2001; 27:27.

    Google Scholar 

  126. Tamaru H, Selker EU. A histone H3 methyltransferase controls DNA methylation in Neurospora crassa. Nature 2001; 414:277–283.

    PubMed  CAS  Google Scholar 

  127. Berger SL, Felsenfeld G. Chromatin goes global. Mol Cell 2001; 8:263–268.

    PubMed  CAS  Google Scholar 

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  1. Department of Cellular Biochemistry and Human Genetics, The Hebrew University — Hadassah Medical School, Jerusalem, Israel

    Aharon Razin

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Razin, A. (2005). DNA Methylation. In: DNA Methylation and Cancer Therapy. Medical Intelligence Unit. Springer, Boston, MA. https://doi.org/10.1007/0-387-27443-X_1

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