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Zinc Finger Proteins pp 47–55Cite as

Synthetic Zinc Finger Transcription Factors

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Part of the book series: Molecular Biology Intelligence Unit ((MBIU))

Abstract

The possibility of using designed transcription factors to control gene expression is highly appealing in view of a wide range of promising applications in research and biomedicine. In the last decade, the efforts of several research groups have clarified the molecular interactions between zinc finger proteins and DNA, generating a “recognition code” that relates amino acids in specific positions of the finger domain to its DNA target sequence. This chapter describes the methods to design artificial zinc finger transcription factors using the “code” and recent novel selection approaches. Efficacy and possible applications of artificial transcription factors are discussed.

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Authors and Affiliations

  1. Istituto Biologia e Patologia Molecolari Sezione di Patologia Molecolare, CNR, Rome, Italy

    Nicoletta Corbi, Valentina Libri & Claudio Passananti

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  1. Nicoletta Corbi
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  2. Valentina Libri
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  3. Claudio Passananti
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Correspondence to Claudio Passananti .

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Editors and Affiliations

  1. Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA

    Shiro Iuchi

  2. Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

    Natalie Kuldell

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Corbi, N., Libri, V., Passananti, C. (2005). Synthetic Zinc Finger Transcription Factors. In: Iuchi, S., Kuldell, N. (eds) Zinc Finger Proteins. Molecular Biology Intelligence Unit. Springer, Boston, MA. https://doi.org/10.1007/0-387-27421-9_9

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