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Application of Spin Labels To Membrane Bioenergetics

Photosynthetic Systems of Higher Plants

  • Chapter
Biomedical EPR, Part A: Free Radicals, Metals, Medicine, and Physiology

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 23))

Abstract

Following a brief overview of photosynthesis and the macro structure of chloroplasts, examples are presented of the use of nitroxyl spin labels and spin probes to investigate the processes of electron transport, proton transport, and oxygen transfer in chloroplasts. pH-sensitive nitroxyl radicals can be observed inside and outside thylakoids under various conditions, such as different levels of light. Oxygen consumption can be measured via the effect of oxygen on line width of the free radical. Lipid-soluble spin probes monitor changes in the biological membranes.

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  1. Department of Biophysics, Faculty of Physics, M. V. Lomonosov Moscow State University, Moscow, 119899, Russia

    Alexander N. Tikhonov

  2. National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, 53226

    Witold K. Subczynski

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    Sandra R. Eaton , Gareth R. Eaton  & Lawrence J. Berliner ,  & 

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Tikhonov, A.N., Subczynski, W.K. (2005). Application of Spin Labels To Membrane Bioenergetics. In: Eaton, S.R., Eaton, G.R., Berliner, L.J. (eds) Biomedical EPR, Part A: Free Radicals, Metals, Medicine, and Physiology. Biological Magnetic Resonance, vol 23. Springer, Boston, MA. https://doi.org/10.1007/0-387-26741-7_8

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