Abstract
The purpose of this study is to identify the best perfusate after blood for maintaining skeletal muscle inotropy, muscle peak oxygen consumption (peak VO2), and oxygen consumption at rest (resting VO2) in in situ isolated canine gastrocnemius-plantaris muscle. Rejuvenated red cells suspended in perfusate at hematocrit 30% and 45%, perfusate contained insulin (100 µU·ml−1), adrenalin (0.3 and 3 ng·ml−1), and noradrenaline (3 ng·ml−1). Insulin significantly augmented resting VO2 and contracting muscle peak VO2, and developed isometric twitch tension at 4 Hz, compared with control. Insulin-induced increase in resting muscle VO2 was abrogated by catecholamines. In addition to insulin and catecholamines, the developed twitch tension increased significantly by 178% with the accompanied increase in flow rate. O2 cost (peak VO2 / tension) significantly decreased by 52%. The developed tension did not correlate with O2 delivery but with flow rate and peak VO2 of contracting muscle. We successfully identified the characteristics of the best perfusate after blood. Our results suggest that the positive inotropy by insulin and catecholamines is attributed partly to an O2 delivery-independent increase in flow to contracting muscle and redistribution of flow within the contracting muscle, which suffered from low perfusion by perfusate containing rejuvenated red cells.
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References
H. Kohzuki, Y. Enoki, K. Matsumura, S. Sakata, and S. Shimizu, Flow-dependent influence of high-O2-affinity erythrocytes on peak VO2 in exercising muscle in situ, J. Appl. Physiol. 80(3), 832–838 (1996).
H. Kohzuki, Y. Enoki, S. Sakata, S. Shimizu, and K. Matsumura, Influence of high O2 affinity red cells on developed tension-VO2 relation in skeletal muscle, (in Japanese) J. Physiol. Soc. Japan 53(Suppl), 13 (1990).
H. Kohzuki, Y. Enoki, S. Shimizu, and S. Sakata, High blood O2 affinity and relationship of O2 uptake and delivery in resting muscle, Respir. Physiol. 92(2), 197–208 (1993).
D. Dawson, M. A. Vincent, E. J. Barrett, S. Kaul, A. Clark, H. Leong-Poi, and J. R. Lindner, Vascular recruitment in skeletal muscle during exercise and hyperinsulinemia assessed by contract ultrasound, Am. J. Physiol. Endocrinol. Metab. 282(3), E714–E720 (2002).
M. G. Clark, S. Rattigan, L. H. Clerk, M. A. Vincent, A. D. H. Clark, J. M. Youd, and J. M. B. Newman, Nutritive and non-nutritive blood flow: rest and exercise, Acta Physiol. Scand. 168(4), 519–530 (2000).
J. H. Williams, and W. S. Barnes, The positive inotropic effect of epinephrine on skeletal muscle: a brief review, Muscle & Nerve 12(12), 968–975 (1989).
R. J. Murphy, P. F. Gardiner, G. Rousseau, M. Bouvier, and L. Beliveau, Chronic beta-blockade increases skeletal muscle beta-adrenergic-receptor density and enhances contractile force, J. Appl. Physiol. 83(2), 459–465 (1997).
J. A. Rall, Energetic aspects of skeletal muscle contraction: Implications of fiber types, Exercise Sport Sci. Rev. 13, 33–74 (1985).
M. Schmitt, P. Meunier, A. Rochas, and J. Chatonnet, Catecholamines and oxygen uptake in dog skeletal muscle in situ, Pflügers Arch. 345(2), 145–158 (1973).
C. D. Marsden, and J. C. Meadows, The effect of epinephrine on the contraction of human muscle, J. Physiol. 207(2), 429–448 (1970).
E. A. Richter, N. B. Ruderman, and H. Galbo, Alpha and beta-adrenergic effects on metabolism in contracting, perfused muscle, Acta Physiol. Scand. 116(3), 215–222 (1982).
B. Folkow, and H. D. Halicka, A comparison between “red” and “white” muscle with respect to blood supply, capillary surface area and oxygen uptake during rest and exercise, Microvasc. Res. 1, 1–14 (1968).
E. Holmberg, and B. Waldeck, The effect of insulin on skeletal muscle contractions and its relation to the effect produced by beta-adrenoceptor stimulation, Acta Physiol. Scand. 109(2), 225–229 (1980).
J. A. Flatman, and T. Clausen, Combined effects of epinephrine and insulin on active electrogenic Na+-K+ transport in rat soleus muscle, Nature 281(5732), 580–581 (1979).
M. T. Crow, and M. J. Kushmerick, Phosphorylation of myosin light chains in mouse fast-twitch muscle associated with reduced actomyosin turnover rate, Science 217(4562), 835–837 (1982).
M. T. Crow, and M. J. Kushmerick, Myosin light chain phosphorylation is associated with a decrease in the energy cost for contraction in fast twitch mouse muscle, J. Biol. Chem. 257(5), 2121–2124 (1982).
J. K. Barclay, A delivery-independent blood flow effect on skeletal muscle fatigue, J. Appl. Physiol. 61(3), 1084–1090 (1986).
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Kohzuki, H., Fujino, H. (2005). Effects of Insulin and Catecholamines on Inotropy and Oxygen Uptake. In: Okunieff, P., Williams, J., Chen, Y. (eds) Oxygen Transport to Tissue XXVI. Advances in Experimental Medicine and Biology, vol 566. Springer, Boston, MA. https://doi.org/10.1007/0-387-26206-7_7
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DOI: https://doi.org/10.1007/0-387-26206-7_7
Publisher Name: Springer, Boston, MA
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