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Rate of Decrease of PO2 from an Arteriole with Arrested Flow

  • Roland N. Pittman
  • Aleksander S. Golub
  • William F. Schleicher
Conference paper
  • 1.4k Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 566)

Abstract

When flow to a region is arrested, the amount of oxygen contained within the stationary blood decreases at a rate dependent on the oxygen utilization of the surrounding tissue. We used phosphorescence quenching microscopy to measure arteriolar PO2 in the mesentery of male Sprague-Dawley rats. Flow was quickly stopped (< 1 s) by occluding the microvessels using an inflatable Saran bag attached to the microscope objective. The rate of decline in PO2 following occlusion yielded a calculated initial flux of oxygen out of the vessel lumen of 8.0 × 10−7 ml O2 cm−2 sec−1. An upper limit on the oxygen consumption of the arteriolar wall was calculated by assuming that all of the oxygen in the lumen was consumed by the wall at the initial rate. This value was 2.5 × 10−3 ml O2 cm−3 sec−1 and is an overestimate since the oxygen consumption of the nearby parenchymal cells was neglected. The calculated maximum oxygen consumption of the wall is more than an order of magnitude smaller than that reported previously for arterioles in the rat mesentery (6.5 × 10−2 ml O2 cm−3 sec−1). We conclude that oxygen consumption of the arteriolar wall is similar to previous values for other vascular tissues.

Keywords

Oxygen Consumption Heart Circ Intravital Microscopy Oxygen Gradient Oxygen Removal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Roland N. Pittman
  • Aleksander S. Golub
  • William F. Schleicher

There are no affiliations available

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