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Benzoate Hydroxylation

A measure of oxidative stress in divers
  • Gernot Gronow
  • Wataru Kähler
  • Andreas Koch
  • Norbert Klause
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 566)

Abstract

Hyperoxia may facilitate the formation of reactive oxygen species. Recent experiments indicated signs of oxidative stress after 3.5 h hyperoxic diving. We analyzed in the urine of healthy, 100% O2-breathing male volunteers before and after 45 min sea-water diving (170 kPa) or 30 min resting at 280 kPa in a pressure chamber (HBO) for sub-fractions of hydroxybenzoate (HB), monohydroxybenzoate (MHB), and of dihydroxybenzoate (DHB). Measurements were performed by HPLC and electrochemical or UV-detection. Additionally, urinary concentrations of thiobarbituric acid-reactive sub-stances (TBARS) and of creatinine (CREA) were analyzed by standard colorimetric assays. During HBO treatment, TBARS, DHB, 2,4-DHB, and 3,4-DHB increased significantly. MHB and CREA did not change. 2,4- and 3,4-DHB-alterations correlated with changes in TBARS. Diving induced urine dilution and stimulated oxygen consumption. Urinary TBARS and HB rose significantly higher during diving at 170 kPa than during HBO at 280 kPa. A different pattern in urinary sub-fractions of DHB could be observed in divers: 2,6 > 2,3 > 2,5 > 3,4. Changes in 2,6- and 2,5-DHB correlated significantly with alterations in TBARS. 2,6-DHB probably indicated renal oxidant stress similar to previously described animal experiments. It is concluded that analyzing urinary HB may provide a sensitive measure to quantify and qualify oxidant stress in divers.

Keywords

Hyperbaric Oxygenation Open Column Black Column Renal Oxidant Stress Erythrocyte Osmotic Fragility 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    J. A. Kazzaz, J. Xu, T. A. Palaia, L. Mantell, A. M. Fein, and S. Horowitz, Cellular oxygen toxicity: oxidant injury without apoptosis, J. Biol. Chem. 271, 15182–15186 (1996).PubMedCrossRefGoogle Scholar
  2. 2.
    A. Y. Shaikh, J. Yu, Y. Wu, L. He, and C. Y. Hsu, Melatonin protects bovine cerebral endothelial cells from hyperoxia induced DNA damage and death, Neurosci. Lett. 229, 193–197 (1997).PubMedCrossRefGoogle Scholar
  3. 3.
    R. L. Morton, D. Ikle, and C. W. White, Loss of lung mitochondrial aconitase activity due to hyperoxia in bronchopulmonary dysplasia in primate, Am. J. Physiol. 274, L127–L134 (1998).PubMedGoogle Scholar
  4. 4.
    S. E. Bearden, S. N. Cheuvront, A. Ring, and E. M. Haymes, Oxidative stress during a 3,5-hour exposure to 120 kPa(a) PO2 in human divers, Undersea Hyper. Med. 26, 159–164 (1999).Google Scholar
  5. 5.
    M. Brichta, L. Hock, J. Plöse, H. Kappus, R. Beneke, and K. Behn, Lipid-peroxide related hemodilution during repetitive hyperbaric oxygenation, Adv. Exp. Med. Biol. 345, 189–194 (1994)PubMedGoogle Scholar
  6. 6.
    P. T. Diaz, Z. W. She, W.-B. Davis, and T. L. Clanton, Hydroxylation of salicylate by the in vitro diaphragm: evidence for hydroxyl radical production during fatigue, J. Appl. Physiol. 75, 540–545 (1993).PubMedGoogle Scholar
  7. 7.
    M. Malyusz, W. Kähler, and G. Gronow, Hippurate metabolism as a hydroxyl radical trapping mechanism in the rat kidney, Kidney Blood Pres. Res. 24, 149–158 (2001).CrossRefGoogle Scholar
  8. 8.
    H. Ohkawa, N. Ohishi, and K. Yagi, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal. Biochem. 95, 351–358 (1979).PubMedCrossRefGoogle Scholar
  9. 9.
    J. M. C. Gutteridge, and B. Halliwell, Free radicals and antioxidants in the year 2000: a historical look to the future, Ann. NY Acad. Sci. 899, 136–147 (2000).PubMedCrossRefGoogle Scholar
  10. 10.
    J. M. Clark, R. M. Jackson, C. J. Lambertsen, R. Gelfand, W. D. Hiller, and M. Unger, Pulmonary function in men after oxygen breathing at 3 ATA for 3.5 h, J. Appl. Physiol. 71, 878–885 (1991).PubMedGoogle Scholar
  11. 11.
    K. H. Muhvich, M. R. Piano, R. A. M. Myers, J. L. Ferguson, and L. Marzella, Hyperbaric oxygenation decreases blood flow in normal and septic rats, Undersea Biomed. Res. 19, 31–40 (1992).PubMedGoogle Scholar
  12. 12.
    J. Anderson and E. Schagatay, Arterial oxygen desaturation during apnea in humans, Undersea Hyperb. Med. 25, 21–35(1998).Google Scholar
  13. 13.
    L. E. Wittmers, Pathophysiology of cold exposure, Minn. Med. 84, 30–36 (2001).PubMedGoogle Scholar
  14. 14.
    S. Morita, S. M. Snider, and Y. Inada, Increased n-pentene excretion in humans: a consequence of pulmonary oxygen exposure, Anesthesiol. 64, 730–733 (1986).CrossRefGoogle Scholar
  15. 15.
    J. M. Weinberg, J. A. Davis, M. Abarzua, and T. Kiani, Relationship between cell ATP and glutathione content and protection by glycine against hypoxic tubular cell injury, J. Lab. Clin. Med. 113, 612–623 (1989).PubMedGoogle Scholar
  16. 16.
    G. Gronow, N. Klause, and M. Mályusz, Support of hypoxic renal cell volume regulation by glycine, Adv. Exp. Med. Biol. 277, 705–712 (1990).PubMedGoogle Scholar
  17. 17.
    G. Gronow, M. Moussavian, and M. Mályusz, Effect of hydroxyl radical scavengers in renal cortical cells, Adv. Exp. Biol. Med. 487, 345–351 (1999).Google Scholar
  18. 18.
    R. A. Floyd, R. Henderson, J. J. Watson, and P. K. Wong, Use of salicylate with high pressure liquid chromatography and electrochemical detection (LCED) as a sensitive measure of hydroxyl free radicals in adriamycin-treated rats, J. Free Radic. Biol. Med. 225, 221–235 (1986).Google Scholar
  19. 19.
    M. Benedetti-Strolin, and K. F. Tipton, Involvement of monooxygenases and amino oxidase in hydroxyl radical generation in vivo, Neurobiol. 7, 123–134 (1999).Google Scholar
  20. 20.
    M. Paolini, A. Antelli, L. Pozetti, D. Spetlova, P. Perocco, L. Valgimigli, G. F. Pedulli, and G. Cantelli-Forti, Induction of cytochrome P450-enzymes and overgeneration of oxygen radicals in betacarotene supplemented rats, Carcinogen. 22, 1483–1495 (2001).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Gernot Gronow
  • Wataru Kähler
  • Andreas Koch
  • Norbert Klause

There are no affiliations available

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