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Developing Strategies for Three-Dimensional Imaging of Oxygen Tension in the Rodent Retina

  • Ross D. Shonat
  • Adam S. Norige
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 566)

Abstract

Insufficient oxygen delivery and retinal hypoxia have been implicated as causal in the development of many devastating diseases of the eye. While the two-dimensional imaging of retinal oxygen tension (PO2) has now been applied in a variety of different animal models, it is fundamentally a luminescence-based system lacking depth discrimination. However, mammalian retinal tissue is nourished by two distinct vascular beds, the retinal and the choroidal vasculatures, and they are exceedingly difficult to separate using traditional two-dimensional imaging strategies. Numerous studies have demonstrated that retinal and choroidal PO2 differ substantially. Therefore, the single PO2 value currently returned through data analysis cannot accurately represent the separate contributions of the choroidal and retinal vasculatures to the state of retinal oxygenation. Such a separation would significantly advance our understanding of oxygen delivery dynamics in these two very distinct vasculatures. In this study, we investigate new strategies for generating separate retinal and choroidal PO2 maps in the rodent retina using our existing phosphorescence-based lifetime imaging system.

Keywords

Diabetic Retinopathy Mouse Retina Retinal Vasculature Choroidal Vessel Phosphorescence Emission 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Ross D. Shonat
  • Adam S. Norige

There are no affiliations available

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