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Cerebral Oxygenation During Repetitive Apnea in Newborn Piglets

  • Gregory Schears
  • Jennifer Creed
  • Tatiana Zaitseva
  • Steven Schultz
  • David F. Wilson
  • Anna Pastuszko
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 566)

Abstract

This study examined the effect of repetitive apnea on brain oxygen pressure in newborn piglets. Each animal was given 10 episodes of apnea, initiated by disconnecting them from the ventilator and completed by reconnecting them to the ventilation circuit. The apneic episodes were ended 30 sec after the heart rate reached the bradycardic threshold of 60 beats per min. The oxygen pressure in the microvasculature of the cortex was measured by oxygen-dependent quenching of the phosphorescence. In all experiments, the blood pressure, body temperature, and heart rate were continuously monitored. Arterial blood samples were taken throughout the experiment and the blood pH, PaO2 and PaCO2 were measured.

During pre-apnea, cortical oxygen was 55.1 ± 6.4 (SEM, n = 7) mm Hg and decreased during each apnea to 8.1 ± 2.8 mm Hg. However, the values of cortical oxygen varied during recovery periods. Maximal oxygen levels during recovery from the first two apneic episodes were 76.8 ± 12 mm Hg and 69.6 ± 9 mm Hg, respectively, values higher than pre-apnea. Cortical oxygen pressure then progressively decreased following consequent apnea.

In conclusion, the data show that repetitive apnea caused a progressive decrease in cortical oxygen levels in the brain of newborn piglets. This deficit in brain oxygenation can be at least partly responsible for the neurological side effects of repetitive apnea.

Keywords

Oxygen Pressure Cerebral Oxygenation Perinatal Asphyxia Newborn Piglet Fetal Sheep 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    K. Barrington, and N. Finer, The natural history of the appearance of apnea of prematurity, Pediatr. Res. 29, 372–375 (1991).PubMedGoogle Scholar
  2. 2.
    R. J. Martin, M. B. Miller, and W. A. Carlo, Pathogenesis of apnea in preterm infants, J. Pediatr. 109, 733–741 (1986).PubMedCrossRefGoogle Scholar
  3. 3.
    A. Pastuszko, S. N. Lajevardi, J. Chen, O. Tammela, D. F. Wilson, and M. Delivoria-Papadopoulos, Effects of graded levels of tissue oxygen pressure on dopamine metabolism in striatum of newborn piglets, J. Neurochem. 60, 161–166 (1993).PubMedGoogle Scholar
  4. 4.
    A. Pastuszko, Metabolic responses of the dopaminergic system during hypoxia in newborn brain, Biochem. Med. Metab. Biol. 51, 1–15 (1994).PubMedCrossRefGoogle Scholar
  5. 5.
    M. Yonetani, Ch-Ch. Huang, N. Lajevardi, A. Pastuszko, M. Delivoria-Papadopoulos, and D. F. Wilson, Effect of hemorrhagic hypotension on extracellular level of dopamine, cortical oxygen pressure and blood flow in brain of newborn piglets, Neurosci. Lett. 180, 247–252 (1994).PubMedCrossRefGoogle Scholar
  6. 6.
    M. Olano, D. Song, S. Murphy, D. F. Wilson, and A. Pastuszko, Relationships of dopamine, cortical oxygen pressure, and hydroxyl radicals in brain of newborn piglets during hypoxia and posthypoxic recovery, J. Neurochem. 65, 1205–1212 (1995).PubMedCrossRefGoogle Scholar
  7. 7.
    W. M. DeCampli, G. Schears, R. Myung, S. Schultz, J. Creed, A. Pastuszko, and D. F. Wilson, Tissue oxygen tension during regional low flow perfusion in neonates, J. Thorac. Cardiovasc. Surg. 125(3 Pt 1), 472–480 (2003).PubMedCrossRefGoogle Scholar
  8. 8.
    S. A. Vinogradov, M. A. Fernandez-Seara, B. W. Dugan, and D. F. Wilson, Frequency domain instrument for measuring phosphorescence lifetime distributions in heterogeneous samples, Rev. Sci. Inst. 72(8), 3396–3306 (2001).CrossRefGoogle Scholar
  9. 9.
    I. B. Rietveld, E. Kim, and S. A. Vinogradov, Dendrimers with tetrabenzoporphyrin cores: near infrared phosphors for in vivo oxygen imaging, Tetrahedron 59(22), 3821–3831 (2003).CrossRefGoogle Scholar
  10. 10.
    I. Dunphy, S. A. Vinogradov, and D. F. Wilson, Oxyphor R2 and G2: Phosphors for measuring oxygen by oxygen dependent quenching of phosphorescence, Analy. Biochem. 310, 191–198 (2002).CrossRefGoogle Scholar
  11. 11.
    R. A. Jones, and D. Lukeman, Apnea of immaturity. 2. Mortality and handicap, Arch. Dis. Child 57, 766–768 (1982).PubMedGoogle Scholar
  12. 12.
    G. A. Levitt, A. Mushin, S. Bellman, and D. R. Harvey, Outcome of preterm infants who suffered neonatal apneic attack, Early Human Dev. 16, 235–243 (1988).CrossRefGoogle Scholar
  13. 13.
    N. R. Kreisman, T. J. Sick, and M. Rosenthal, Importance of vascular responses in determining cortical oxygenation during recurrent paroxysmal events of varying duration and frequency of repetition, J. Cereb. Blood Flow Metab. 3, 330–338 (1983).PubMedGoogle Scholar
  14. 14.
    S. Tomida, T. S. Nowak, K. Vass, J. M. Lohr, and I. Klatzo, Experimental model of repetitive ischemic attacks in the gerbil, J. Cereb. Blood Flow Metab. 7, 773–782 (1987).PubMedGoogle Scholar
  15. 15.
    E. C. Mallard, C. E. Williams, A. J. Gunn, M. I. Gunning, and P. D. Gluckman, Frequent episodes of brief ischemia sensitize the fetal sheep brain to neuronal loss and induce striatal injury, Pediatr. Res. 33(1), 61–65 (1993).PubMedGoogle Scholar
  16. 16.
    V. Fellman, and K. O. Raivio, Reperfusion injury as the mechanism of brain damage after perinatal asphyxia, Pediatr. Res. 41, 599–606 (1997).PubMedGoogle Scholar
  17. 17.
    C. Palmer, Hypoxic-ischemic encephalopathy. Therapeutic approaches against microvascular injury, and role of neutrophils, PAF, and free radicals, Clin. Perinatal. 22, 481–517 (1995).Google Scholar
  18. 18.
    A. A. Rosenberg, E. Murdaugh, and C. W. White, The role of oxygen free radicals in postasphyxia cerebral hypoperfusion in newborn lambs, Pediatr. Res. 26, 215–219 (1989).PubMedGoogle Scholar
  19. 19.
    B. R. Karlsson, B. Grögaard, B. Gerdin, and P. A. Steen, The severity of postischemic hypoperfusion increases with duration of cerebral ischemia in rats, Acta Anaesthesiol. Scand. 38, 248–253 (1994).PubMedCrossRefGoogle Scholar
  20. 20.
    R. Pluta, A. S. Lossinsky, H. M. Wisniewski, and M. J. Mossakowski, Early blood-brain barrier changes in the rat following transient complete cerebral ischemia induced by cardiac arrest, Brain Res. 633, 41–52(1994).PubMedCrossRefGoogle Scholar
  21. 21.
    C. K. Petito, W. A. Pulsinelli, G. Jacobson, and F. Plum, Edema and vascular permeability in cerebral ischemia: Comparison between ischemic neuronal damage and infarction, J. Neuropathol. Exp. Neurol. 41, 423–436(1982).PubMedGoogle Scholar
  22. 22.
    R. L. Zhang, M. Chopp, H. Chen, and J. H. Garcia, Temporal profile of ischemic tissue damage, neutrophil response and vascular plugging following permanent and transient middle cerebral artery occlusion in the rat, J. Neurol. Sci. 125, 3–10 (1994).PubMedCrossRefGoogle Scholar
  23. 23.
    W. S. Thomas, E. Mori, B. R. Copeland, J. Q. Yu, J. H. Morrissey, and G. J. del Zoppo, Tissue factor contributes to microvascular defects after focal cerebral ischemia, Stroke 24, 847–854 (1993).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Gregory Schears
  • Jennifer Creed
  • Tatiana Zaitseva
  • Steven Schultz
  • David F. Wilson
  • Anna Pastuszko

There are no affiliations available

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