Abstract
Although several new antiepileptic drugs (AEDs) have been launched during the last two decades, pharmacoresistance of epilepsy is still a major problem in clinical neurology. About 30% of all epileptic patients do not adequately respond to drug treatment.1 In adults, temporal lobe epilepsy (TLE) with complex-partial seizures has the poorest prognosis with up to 70% of the patients being resistant to treatment with available AEDs.2 Although several risk factors for intractability have been identified, the mechanisms underlying intractable epilepsy have not been fully enlightened. Recent data gave evidence that pharmacodynamic mechanisms, i.e., changes in drug targets (target hypothesis),3 as well as pharmacokinetic mechanisms, i.e., a local decrease in brain access of AEDs mediated by overexpression of multidrug transporters (multidrug transporter hypothesis),4 may be involved. Investigations in animal models of pharmacoresistant epilepsy are helpful to increase the understanding of the underlying basis of intractability, i.e., to further substantiate the existing hypotheses, to define further mechanisms, and to examine how different mechanisms coact. Unfortunately, there are only few animal models of pharmacoresistant epilepsy. One of the most extensively characterized animal models in this respect is a pharmacoresistant subgroup of amygdala-kindled Wistar rats.
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8. References
P. Kwan and M.J. Brodie, Refractory epilepsy a progressive, intractable but preventable condition? Seizure 11(2), 77–84 (2002).
I.E. Leppik, Intractable epilepsy in adults, in: Surgical treatment of epilepsy, edited by W.. H. Theodore, (Elsevier, Amsterdam, 1992) pp. 7–11.
S. Remy, S. Gabriel, B.W. Urban, D. Dietrich, T.N. Lehmann, C.E. Elger, U. Heinemann, and H. Beck, A novel mechanism underlying drug resistance in chronic epilepsy, Ann. Neurol. 53(4): 469–479 (2003).
W. Löscher and H. Potschka, Role of multidrug transporters in pharmacoresistance to antiepileptic drugs, J. Pharmacol. Exp. Therap. 301(1), 7–14 (2002).
W. Löscher, R. Jäckel, R., S.J. Czuczwar, Is amygdala kindling in rats a model for drug-resistant partial epilepsy? Exp. Neurol. 93(1), 211–226 (1986).
W. Löscher and C. Rundfeldt, Kindling as a model of drug-resistant partial epilepsy: selection of phenytoin-resistant and nonresistant rats, J. Pharmacol. Exp. Therap. 258(2), 483–489 (1991).
W. Löscher, Animal models of intractable epilepsy, Prog. Neurobiol. 53(2), 239–258 (1997)
W. Löscher, Animal models of drug-resistant epilepsy, NovartisFound. Symp. 243, 149–159 (2002)
U. Ebert and W. Löscher, Characterization of phenytoin-resistant kindled rats, a new model of drug-resistant partial epilepsy: influence of genetic factors, Epilepsy Res. 33(2–3), 217–226 (1999).
S. Cramer, U. Ebert, and W. Löscher, Characterization of phenytoin-resistant kindled rats, a new model of drug-resistant partial epilepsy: comparison of inbred strains, Epilepsia 39(10), 1046–1053 (1998).
W. Löscher, S. Cramer, and U. Ebert, Selection of phenytoin responders and nonresponders in male and female amygdala-kindled Sprague-Dawley rats. Epilepsia 39(11), 1138–1147, (1998)
W. Löscher, E Reissmüller, and U. Ebert, Kindling alters the anticonvulsant efficacy of phenytoin in Wistar rats, Epilepsy Res. 39(3), 211–220 (2002).
H. Beck, R. Steffens, U. Heinemann, and C E. Elger, Properties of voltage-activated Ca2+ currents in acutely isolated human hippocampal granule cells, J. Neurophysiol. 77(3), 1526–1537 (1997).
H. Beck, R. Steffens, C.E. Elger, U. Heinemann, Voltage dependent Ca2+ currents in epilepsy, Epilepsy Res. 32(1–2), 321–332 (1998).
G. Reckziegel, H. Beck, J. Schramm, C.E. Elger, and B.W. Urban, Electrophysiological characterization of Na2+ currents in acutely isolated human hippocampal dentate granule cells, J. Physiology 509, 139–150 (1998).
M. Jeub, H. Beck, E. Siep, C. Rüschenschmidt, E.J. Speckmann, U. Ebert, H. Potschka, C. Freichel, E. Reissmüller, W. Löscher, Effect of phenytoin on sodium and calcium currents in hippocampal CA1 neurons of phenytoin-resistant kindled rats, Neuropharmacology 42(1), 107–116 (2002)
DM. Tishler, K.I. Weinberg, D.R. Hinton, N Barbaro, G.M. Annett, and C. Raffel, 1995, MDR1 gene expression in brain of patients with intractable epilepsy, Epilepsia 36(1), 1–6 (1995)
S.M. Sisodiya, J. Heffeman, and M V. Squier, Over-expression of P-glycoprotein in malformations of cortical development, Neuroreport 10(16), 3437–3441 (1999)
S.M. Sisodiya, W.R Lin, B.N. Harding, M.V. Squier, and M. Thorn, Drug resistance in epilepsy: expression of drug resistance proteins in common causes of refractory epilepsy, Brain 125: 22–31 (2002)
E. Aronica, J.A. Gorter, G.H. Jansen, C.W.M. van Veelen, P.C. van Rijen, S. Leenstra, M. Ramkema, G.L. Scheffer, R.J. Scheper, and D. Troost, Expression and cellular distribution of multidrug transporter proteins in two major causes of medically intractable epilepsy: Focal cortical dysplasia and glioneuronal tumors, Neuroscience 118(2), 417–429 (2003)
S M. Dombrowski, S.Y. Desai, M. Marroni, L. Cucullo, K. Goodrich, W. Bingaman, M.R. Mayberg, L. Bengez, D. Janigro, Overexpression of multidrug resistance genes in endothelial cells from patients with refractory epilepsy, Epilepsia 42(1), 1501–1506 (2001)
H. Potschka, S. Baltes, and W. Löscher, Inhibition of multidrug transporters by verapamil and probenecid does not alter blood-brain barrier penetration of levetiracetam in rats, Epilepsy Res., 58(2–3), 85–91 (2004).
HA. Volk, H. Potschka, and W. Löscher, Increased expression of the multidrug transporter p-glycoprotein in brain capillary endothelial cells and brain parenchyma of amygdala kindled rats, Epilepsy Res. 58(1), 67–79 (2004)
U. Seegers, H. Potschka, and W. Löscher, Expression of the multidrug transporter p-glycoprotein in brain capillary endothelial cells and brain parenchyma of amygdala-kindled rats, Epilepsia 43(7), 675–684 (2002)
A. Siddiqui, R. Kerb, ME. Weale, U. Brinkmann, A. Smith, D.B. Goldstein, N.W. Wood, and S.M. Sisodiya, Association of multidrug resistance in epilepsy with a polymorphism in the drug transporter gene ABCB1, New Engl. J. Medicine 348(15), 1442–1448 (2003).
J. Gu, B.A. Lynch, H. Klitgaard, S. Lu, M. Elashoff, U. Ebert, H. Potschka, and W. Löscher, The antiepileptic drug levetiracetam selectively modifies kindling-induced alterations in gene expression in the temporal lobe of rats, Eur. J. Neurosci. 19(2), 334–345 (2004).
M T. Kimura, S. Irie, S. Shoji-Hoshino, J. Mukai, D. Nadano, M. Oshimura, and T.A. Sato, 14-3-3 is involved in p75 neurotrophin receptor-mediated signal transduction, J. Biol. Chem. 276(20), 17291–17300 (2001).
R.D. York, H. Yao, T. Dillon, C.L. Ellig, S.P. Eckert, E.W. McCleskey, and P.J.S. Stork, Rapl mediates sustained MAP kinase activation induced by nerve growth factor, Nature 392, 622–626 (1998)
S. Shinoda, C.K. Schindler, J. Quan-Lan, J.A. Saugstad, W. Taki, R.P. Simon, and D. C., 2003, Interaction of 14-3-3 with Bid during seizure-induced neuronal death, J. Neurochem. 86(2), 460–469 (2003)
K. Ye, DA Compton, MM. Lai, L.D. Walensky, and S.H. Snyder, 1999, Protein 4.1N binding to nuclear mitotic apparatus protein in PC12 cells mediates the antiproliferative actions of nerve growth factor, J. Neurosci. 19(24), 10747–10756 (1999)
D.H. Lowenstein, Recent advances related to basic mechanisms of epileptogenesis. Epilepsy Res. (Suppl.) 11, 45–60 (1996)
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Potschka, H., Löscher, W. (2005). Mechanisms of Pharmacoresistance in the Phenytoin-Resistant Kindled Wistar Rat. In: Corcoran, M.E., Moshé, S.L. (eds) Kindling 6. Advances in Behavioral Biology, vol 55. Springer, Boston, MA. https://doi.org/10.1007/0-387-26144-3_31
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DOI: https://doi.org/10.1007/0-387-26144-3_31
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