Abstract
A Micro-Electrode Array (MEA) based neuronal spike recording system is described. MEAs are cell-culture dishes with multiple embedded micro-electrodes on their surface. Multi-site recording of electrical signals enable us of visualizing spatially propagating activity patterns in cultured neuronal networks. Here in this chapter, we first introduce basic properties of MEAs, from the viewpoints of their technically advantageous features. Then some examples of actual recording are shown. Monitoring of spontaneous activity during development of cortical networks demonstrates their capability of long-term non-invasive recording. Characteristics of evoked responses can be modified by repetitive electrical stimulation through the embedded electrodes. Based on the high temporal resolution of this system, spatially propagating action potentials in a single neuron are visualized. Finally, combination of MEA recording with fluorescence imaging is illustrated.
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Jimbo, Y., Kasai, N., Torimitsu, K., Tateno, T. (2006). MEA-Based Spike Recording in Cultured Neuronal Networks. In: Xing, WL., Cheng, J. (eds) Frontiers in Biochip Technology. Springer, Boston, MA. https://doi.org/10.1007/0-387-25585-0_4
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DOI: https://doi.org/10.1007/0-387-25585-0_4
Publisher Name: Springer, Boston, MA
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